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Cell No. : Cell Name
RCB1811 : ΔBLM-DT40  update : 2022/04/07
CommentSubline of DT40 cell line, lacking BLM (Bloom syndrome gene) expression.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
RIKEN Genomic Sciences Center
Protein Research Group
1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa, 230-0045 Japan
Dr.MATSUMOTO Takehisa
Fax. +81-45-503-9653
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takeda, Shunichi
Originator Matsumoto, Takehisa
Year of deposit 2002
Original cell DT40
Animal avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene Blast, His
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T25
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10^(5) cells/ml
Passage ratio 1 : 10-20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
3559  Imamura O, Fujita K, Shimamoto A, Tanabe H, Takeda S, Furuichi Y, Matsumoto T.  Bloom helicase is involved in DNA surveillance in early S phase in vertebrate cells.  Oncogene  2001  20(10):1143-51  PubMed ID: 11313858   DOI: 10.1038/sj.onc.1204195

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User's Publication
16932  Manami Watanabe, Yuta Kamada, Khosuke Miyazaki, Shoko Mizoguchi, Keiichi Matsuzaki, Susumu Kitanaka, Shohei Miyata  20-O-IngenolEZ, a catalytic topoisomerase II inhibitor, specifically inhibits cell proliferation and induces double-strand DNA breaks in BLM-/- cells  Med Chem Comm  2011  2:824    DOI: 10.1039/c0md00252f



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