Cell No. : Cell Name
update : 2023/01/04
Comment Rat cells derived from kidney carcinoma. After passage 2. Wistar rat.
Comment from the depositor
Terms and conditions A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Nagoya City University
Order Form
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see ). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp ).
Basic information
Depositor
Tsuda, Hiroyuki
Originator
Tokuzen, Reiko
Year of deposit
2001
Animal
rat
< Mammals
Strain name
Wistar
Gender
Male
Tissue
kidney
Classification
cancer
Year of origin
1994
Lifespan
infinite
Morphology
epithelial-like
Cellosaurus(Expasy)
CVCL_U199
deposit info lot info
Medium
Medium List
Culture type
Adherent cells
Medium and additives
RPMI1640 + 10% FBS
Antibiotics
Free
Passage method
0.25% Trypsin
Culture information
Passage ratio
1 : 4 split
SC frequency
Subculture : 2 times/week
Temperature
37
℃
CO2 concentration
5
%
Freeze medium
Medium + 10% DMSO
Freezing method
Slow freezing
Mycoplasma
(-)
Animal PCR
OK
Isozyme
LD, NP, G6PD, AST
Images
deposit info lot info
Reference information
Reference
0
User's Publication
0
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