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Cell No. : Cell Name
RCB1806 : BP36B(R2)  update : 2023/01/04
CommentRat cells derived from kidney carcinoma. After passage 2. Wistar rat.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Nagoya City University

1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan
Dr.TSUDA Hiroyuki
Fax. +81-52-853-8996
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Tsuda, Hiroyuki
Originator Tokuzen, Reiko
Year of deposit 2001
Animal _rat < Mammals
Strain name Wistar
Gender Male
Tissue kidney
Classification cancer
Year of origin 1994
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U199
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD, AST
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3087  Tokuzen R, Iwahori Y, Asamoto M, Iigo M, Hasegawa F, Satoh T, Ishidate M Jr, Tsuda H  Establishment and characterization of three new rat renal cell carcinoma cell lines from N-ethyl-N-hydroxyethylnitrosamine-induced basophilic cell tumors.  Pathol Int  2001  51:65-71  PubMed ID: 11169143   DOI: 10.1046/j.1440-1827.2001.01167.x

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