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Cell No. : Cell Name
RCB1792 : SIL-6/1  update : 2024/01/17
CommentSubline of A-6. Dependent on SCF and IL-6 for proliferation. Differentiate to erythroid cells by IL-3.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Atsumi, Tadao
Originator Atsumi, Tadao
Year of deposit 2001
Original cell A-6
Animal _mouse < Mammals
Strain name C3H
Gender Male
Age at sampling embryo
Tissue embryo
Classification other
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U118
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium DMEM/HamF12 + 15% FBS + 10μg/ml Insulin + 10μg/ml Transferrin + 0.1mM 2-Mercaptoethanol + 10ng/ml SCF + 25ng/ml IL-6
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 0
User's Publication 0


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