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Cell No. : Cell Name
RCB1791 : PCC3/A/1  update : 2022/04/07
CommentMouse teratoma-derived cell line.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Atsumi, Tadao
Originator Atsumi, Tadao
Year of deposit 2001
Animal mouse < Mammals
Strain name 129
Gender Male
Age at sampling embryo
Tissue embryo
Disease name teratocarcinoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E064
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 1


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Reference
2812  Miwa Y, Atsumi T, Imai N, Ikawa Y  Primitive erythropoiesis of mouse teratocarcinoma stem cells PCC3/A/1 in serum-free medium.  Development  1991  111:543-9  PubMed ID: 1893874  

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User's Publication
6391  Fujitani N, Takegawa Y, Ishibashi Y, Araki K, Furukawa J, Mitsutake S, Igarashi Y, Ito M, Shinohara Y.  Qualitative and quantitative cellular glycomics of glycosphingolipids based on rhodococcal endoglycosylceramidase-assisted glycan cleavage, glycoblotting-assisted sample preparation, and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry analysis.  J Biol Chem  2011  286(48):41669-79  PubMed ID: 21965662   DOI: 10.1074/jbc.M111.301796



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