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Cell No. : Cell Name
RCB1790 : P19C6  update : 2023/01/05
CommentEmbryonal carcinoma cell line derived from mouse C3H. Differentiate to nervous system.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Atsumi, Tadao
Originator Atsumi, Tadao
Year of deposit 2001
Original cell P19
Animal _mouse < Mammals
Strain name C3H
Gender Male
Age at sampling embryo
Tissue embryonal carcinoma
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_L988
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 3
User's Publication 5

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2834  Gao X, Bian W, Yang J, Tang K, Kitani H, Atsumi T, Jing N  A role of N-cadherin in neuronal differentiation of embryonic carcinoma P19 cells  Biochem Biophys Res Commun.  2001  284:1098-1103  PubMed ID: 11414696   DOI: 10.1006/bbrc.2001.5089
4962  McBurney MW, Rogers BJ.  Isolation of male embryonal carcinoma cells and their chromosome replication patterns.  Dev Biol  1982  89(2):503-8  PubMed ID: 7056443   DOI: 10.1016/0012-1606(82)90338-4
4963  McBurney MW, Jones-Villeneuve EM, Edwards MK, Anderson PJ.  Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line.  Nature  1982  299(5879):165-7  PubMed ID: 7110336   DOI: 10.1038/299165a0

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User's Publication
11771  Hanafusa K, Hayashi N.  The Flot2 component of the lipid raft changes localization during neural differentiation of P19C6 cells.  BMC Mol Cell Biol  2019    PubMed ID: 31455216   DOI: 10.1186/s12860-019-0225-0
12658  Tabe S, Hikiji H, Ariyoshi W, Hashidate-Yoshida T, Shindou H, Okinaga T, Shimizu T, Tominaga K, Nishihara T.  Lysophosphatidylethanolamine acyltransferase 1/membrane-bound O-acyltransferase 1 regulates morphology and function of P19C6 cell-derived neurons  FASEB J  2016  30(7):2591-601  PubMed ID: 27048541   DOI: 10.1096/fj.201500097R
14090  Siew S, Kaneko M, Mie M, Kobatake E.  Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation  J Mater Chem B  2016  4(14):2512-2518  PubMed ID: 32263200   DOI: 10.1039/c5tb01579k
10356  Takanobu Kobayashi, Rie Komori, Kiyoshi Ishida, Katsuhito Kino, Sei-ichi Tanuma, Hiroshi Miyazawa  Tal2 Expression Is Induced by All-Trans Retinoic Acid in P19 Cells Prior to Acquisition of Neural Fate  Sci Rep  2014  4:4935  PubMed ID: 24816818   DOI: 10.1038/srep04935
8424  Wakabayashi T, Kosaka J, Mochii M, Miki Y, Mori T, Takamori Y, Yamada H.  C38, equivalent to BM88, is developmentally expressed in maturing retinal neurons and enhances neuronal maturation.  J. Neurochem.  2010  112:1235-48  PubMed ID: 20002527   DOI: 10.1111/j.1471-4159.2009.06536.x

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