Cell No. : Cell Name
RCB1790 : P19C6
update : 2024/06/19
|
| Comment | Embryonal carcinoma cell line derived from mouse C3H. Differentiate to nervous system. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
|
Basic information
|
Depositor |
Atsumi, Tadao
|
| Originator |
Atsumi, Tadao
|
| Year of deposit |
2001
|
| Original cell |
P19
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H
|
| Gender |
Male
|
| Age at sampling |
embryo
|
| Tissue |
embryonal carcinoma
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_L988
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 15% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4-8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
3
|
| User's Publication |
7
|
| Reference |
|
2834
Gao X, Bian W, Yang J, Tang K, Kitani H, Atsumi T, Jing N
A role of N-cadherin in neuronal differentiation of embryonic carcinoma P19 cells
Biochem Biophys Res Commun.
2001
284:1098-1103
PubMed ID: 11414696
DOI: 10.1006/bbrc.2001.5089
|
|
4962
McBurney MW, Rogers BJ.
Isolation of male embryonal carcinoma cells and their chromosome replication patterns.
Dev Biol
1982
89(2):503-8
PubMed ID: 7056443
DOI: 10.1016/0012-1606(82)90338-4
|
|
4963
McBurney MW, Jones-Villeneuve EM, Edwards MK, Anderson PJ.
Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line.
Nature
1982
299(5879):165-7
PubMed ID: 7110336
DOI: 10.1038/299165a0
|
| User's Publication |
|
19814
Maeda T.
Detection of joining points for genomic DNA circularization in cell culture
Can J Physiol Pharmacol
2023
101(9):481-487
PubMed ID: 37339525
DOI: 10.1139/cjpp-2022-0461
|
|
11771
Hanafusa K, Hayashi N.
The Flot2 component of the lipid raft changes localization during neural differentiation of P19C6 cells.
BMC Mol Cell Biol
2019
PubMed ID: 31455216
DOI: 10.1186/s12860-019-0225-0
|
|
12658
Tabe S, Hikiji H, Ariyoshi W, Hashidate-Yoshida T, Shindou H, Okinaga T, Shimizu T, Tominaga K, Nishihara T.
Lysophosphatidylethanolamine acyltransferase 1/membrane-bound O-acyltransferase 1 regulates morphology and function of P19C6 cell-derived neurons
FASEB J
2016
30(7):2591-601
PubMed ID: 27048541
DOI: 10.1096/fj.201500097R
|
|
14090
Siew S, Kaneko M, Mie M, Kobatake E.
Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation
J Mater Chem B
2016
4(14):2512-2518
PubMed ID: 32263200
DOI: 10.1039/c5tb01579k
|
|
19239
Kobayashi T, Suzuki M, Morikawa M, Kino K, Tanuma S, Miyazawa H.
Transcriptional regulation of Tal2 gene by all-trans retinoic acid (atRA) in P19 cells
Biol Pharm Bull
2015
38(2):248-56.
PubMed ID: 25482166
DOI: 10.1248/bpb.b14-00617
|
|
10356
Takanobu Kobayashi, Rie Komori, Kiyoshi Ishida, Katsuhito Kino, Sei-ichi Tanuma, Hiroshi Miyazawa
Tal2 Expression Is Induced by All-Trans Retinoic Acid in P19 Cells Prior to Acquisition of Neural Fate
Sci Rep
2014
4:4935
PubMed ID: 24816818
DOI: 10.1038/srep04935
|
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8424
Wakabayashi T, Kosaka J, Mochii M, Miki Y, Mori T, Takamori Y, Yamada H.
C38, equivalent to BM88, is developmentally expressed in maturing retinal neurons and enhances neuronal maturation.
J. Neurochem.
2010
112:1235-48
PubMed ID: 20002527
DOI: 10.1111/j.1471-4159.2009.06536.x
|
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