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Cell No. : Cell Name
RCB1788 : AT805  update : 2022/04/07
CommentMouse teratoma-derived cell line. Parent cell line of ATDC5.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Atsumi, Tadao
Originator Atsumi, Tadao
Year of deposit 2001
Original cell SEBIII
Animal mouse < Mammals
Strain name 129
Gender Male
Age at sampling embryo
Tissue embryo
Disease name teratocarcinoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8068
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 15% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
2605  Takeichi M, Atsumi T, Yoshida C, Uno K, Okada, T S  Selective adhesion of embryonal carcinoma cells and differentiated cells by Ca2+-dependent sites.  Dev Growth Differ  1981  87:340-350  PubMed ID: 6793433   DOI: 10.1016/0012-1606(81)90157-3

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