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Cell No. : Cell Name
RCB1773 : Lu-143  update : 2021/02/26
CommentHuman cell line derived from lung cancer. Small cell carcinoma. Application consideration
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Terasaki, Takeo
Originator Terasaki, Takeo
Year of deposit 2001
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 60 years
Tissue lung
Disease name Terasaki, Takeo
Metastastatic ability Yes
Metastatsd tissue lymph node
Classification cancer
Year of origin 1987
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 5
User's Publication 2


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Reference
1974  Terasaki T, Matsuno Y, Shimosato Y, Yamaguchi K, Ichinose H, Nagatsu T, Kato K  Establishment of a human small cell lung cancer cell line producing a large amount of anti-diuretic hormone.  Jpn J Cancer Res (--> Cancer Sci)  1994  85:718-22  PubMed ID: 8071114
3143  Sameshima Y, Matsuno Y, Hirohashi S, Shimosato Y, Mizoguchi H, Sugimura T, Terada M, Yokota J  Alterations of the p53 gene are common and critical events for the maintenance of malignant phenotypes in small-cell lung carcinoma.  Oncogene  1992  7:451-7  PubMed ID: 1312700
3147  Miller, C W, Simon K, Aslo A, Kok K, Yokota J, Buys, C H, Terada M, Koeffler, H P  p53 mutations in human lung tumors.  Cancer Res  1992  52:1695-8  PubMed ID: 1312896
3146  Sameshima Y, Akiyama T, Mori N, Mizoguchi H, Toyoshima K, Sugimura T, Terada M, Yokota J  Point mutation of the p53 gene resulting in splicing inhibition in small cell lung carcinoma.  Biochem Biophys Res Commun  1990  173:697-703  PubMed ID: 2175605
1111  Yokota J, Akiyama T, Fung, Y K, Benedict, W F, Namba Y, Hanaoka M, Wada M, Terasaki T, Shimosato Y, Sugimura T, et al.  Altered expression of the retinoblastoma (RB) gene in small-cell carcinoma of the lung.  Oncogene  1988  3:471-5  PubMed ID: 2856251

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User's Publication
9502  Morita M, Sato T, Nomura M, Sakamoto Y, Inoue Y, Tanaka R, Ito S, Kurosawa K, Yamaguchi K, Sugiura Y, Takizaki H, Yamashita Y, Katakura R, Sato I, Kawai M, Okada Y, Watanabe H, Kondoh G, Matsumoto S, Kishimoto A, Obata M, Matsumoto M, Fukuhara T, Motohashi H, Suematsu M, Komatsu M, Nakayama KI, Watanabe T, Soga T, Shima H, Maemondo M, Tanuma N.  PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth.  Cancer Cell  2018  33:355-367.e7  PubMed ID: 29533781
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309



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