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Cell No. : Cell Name
RCB1766 : ΔligIV-DT40  update : 2024/01/17
CommentSubline of DT40 cell line, lacking ligase IV expression.
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Proc Natl Acad Sci USA 2001;98:12109-121113) .
Remarks
approver's address
×
English
Address
Yokohama city university

Maioka-cho 641-12, Totsuka-ku, Yokohama 244-0813 Japan
Dr.ADACHI Noritaka
Fax. +81-45-620-1901
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Adachi, Noritaka
Originator Koyama, Hideki
Year of deposit 2001
Original cell DT40
Animal _avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene lacking ligase IV
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T44
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 10-20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information Reference 2
User's Publication 0


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Reference
17845  Sharma V, Collins LB, Chen TH, Herr N, Takeda S, Sun W, Swenberg JA, Nakamura J.  Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations  Oncotarget  2016  7(18):25377-90  PubMed ID: 27015367   DOI: 10.18632/oncotarget.8298
3553  Adachi N, Ishino T, Ishii Y, Takeda S, Koyama H.  DNA ligase IV-deficient cells are more resistant to ionizing radiation in the absence of Ku70: Implications for DNA double-strand break repair.  Proc Natl Acad Sci U S A  2001  98(21):12109-13  PubMed ID: 11593023   DOI: 10.1073/pnas.201271098

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