Cell No. : Cell Name RCB1764 : ΔRev3-DT40  update : 2022/04/07
Comment			Subline of DT40 cell line, lacking Rev3 expression.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Takeda, Shunichi
		Originator	Takata, Minoru
		Year of deposit	2001
		Original cell	DT40
		Animal	avian < Birds
		Tissue	B cell
		Classification	mutant
		Recombinant	recombinant
		Exogene	pKO-Rev3-his, pKO-Rev3-bsr
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_1T51
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Medium and additives		RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage cell No		1.5-2 x 10^(5) cells/ml
		Passage ratio		1 : 10-20 split
		SC frequency		Subculture : every 2 days
		Temperature		39.5 ℃
		CO2 concentration		5 %
		Freeze medium		90% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Others		Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information		Reference	2
		User's Publication	0

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Reference

3388  Okada, Takashi, Sonoda, Eiichiro, Yoshimura, Michio, Kawano, Yoshiaki, Saya, Hideyuki, Kohzaki, Masaoki, Takeda, Shunichi  Multiple roles of vertebrate REV genes in DNA repair and recombination.  Mol Cell Biol  2005  25:6103-11  PubMed ID: 15988022   DOI: 10.1128/MCB.25.14.6103-6111.2005

3435  Mizutani A, Okada T, Shibutani S, Sonoda E, Hochegger H, Nishigori C, Miyachi Y, Takeda S, Yamazoe M.  Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells.  Cancer Res.   2004  64(9):3144-7  PubMed ID: 15126352

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