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Cell No. : Cell Name
RCB1757 : HS-P  update : 2022/04/07
CommentRat cell line derived from histiocytic sarcoma. Possessing characteristics of macrophage.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamate, Jyoji
Originator Yamate, Jyoji
Year of deposit 2001
Animal rat < Mammals
Strain name F344/DuCrj
Gender Male
Age at sampling 24 months
Tissue liver
Disease name histiocytic sarcoma
Metastastatic ability Yes
Metastatsd tissue lung
Classification cancer
Year of origin 1996
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_5291
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, G6PD
Reference information Reference 5
User's Publication 0


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Reference
2783  Yamate J, Maeda M, Benn SJ, Laithwaite JE, Allan A, Ide M, Kuwamura M, Kotani T, Sakuma S, Lamarre J  Differential effects of transforming growth factor-beta1, a fibrogenic factor, on macrophage-like cells (HS-P) and myofibroblastic cells (MT-9) in vitro.  Toxicol Pathol  2001  29:483-91  PubMed ID: 11560254
2784  Yamate J, Maeda M, Benn SJ, Laithwaite JE, Allan A, Ide M, Kuwamura M, Kotani T, Sakuma S, LaMarre J  Effects of lipopolysaccharide on a macrophage-like cell line (HS-P) from a rat histiocytic sarcoma.  J Comp Pathol  2001  125:15-24  PubMed ID: 11437512
2785  Yamate J, Maeda M, Tsukamoto Y, Benn SJ, Laithwaite JE, Allan A, Kannan Y, Ide M, Kuwamura M, Kotani T, Sakuma S, LaMarre J  Macrophage-like cell line (HS-P) from a rat histiocytic sarcoma.  J Comp Pathol  2001  124:183-91  PubMed ID: 11222016
3477  J E Laithwaite , S J Benn, J Yamate, D J FitzGerald, J LaMarre  Enhanced macrophage resistance to Pseudomonas exotoxin A is correlated with decreased expression of the low-density lipoprotein receptor-related protein  Infect Immun  1999  67(11):5827-3  PubMed ID: 10531236
3478  Yoko Mor, Takeshi Izawa, Shigeo Takenaka, Mitsuru Kuwamura, Jyoji Yamate  Participation of functionally different macrophage populations and monocyte chemoattractant protein-1 in early stages of thioacetamide-induced rat hepatic injury  Toxicol Pathol  2009  37(4):463-73  PubMed ID: 19389872

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