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Cell No. : Cell Name
RCB1755 : KMY-2  update : 2022/04/07
CommentSubline of KMY-J. Epithelial-like.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yamate, Jyoji
Originator Yamate, Jyoji
Year of deposit 2001
Original cell KMY-J
Animal rat < Mammals
Strain name F344/DuCrj
Gender Female
Age at sampling 24 months
Tissue meninx
Disease name malignant meningioma
Metastastatic ability Yes
Metastatsd tissue lung
Classification cancer
Year of origin 1994
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_5303
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
Reference information Reference 2
User's Publication 0


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Reference
3483  J Yamate, S Ishimine, T Izawa, D Kumagai, M Kuwamura  Macrophage populations and expressions of regulatory proinflammatory factors in the rat meninx under lipopolysaccharide treatment in vivo and in vitro  Histol Histopathol  2009  24(1):13-24  PubMed ID: 19012240   DOI: 10.14670/HH-24.13
2782  Tsujino K, Yamate J, Tsukamoto Y, Kumagai D, Kannan Y, Jippo T, Kuwamura M, Kotani T, Takeya M, Sakuma S  Establishment and characterization of cell lines derived from a transplantable rat malignant meningioma: morphological heterogeneity and production of nerve growth factor.  Acta Neuropathol  1997  93:461-70  PubMed ID: 9144584  

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