Cell No. : Cell Name RCB1755 : KMY-2  update : 2023/01/06
Comment			Subline of KMY-J. Epithelial-like.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Yamate, Jyoji
		Originator	Yamate, Jyoji
		Year of deposit	2001
		Original cell	KMY-J
		Animal	_rat < Mammals
		Strain name	F344/DuCrj
		Gender	Female
		Age at sampling	24 months
		Tissue	meninx
		Disease name	malignant meningioma
		Metastatic ability	Yes
		Metastatic tissue	lung
		Classification	cancer
		Year of origin	1994
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_5303
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP, AST
Reference information		Reference	2
		User's Publication	0

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Reference

3483  J Yamate, S Ishimine, T Izawa, D Kumagai, M Kuwamura  Macrophage populations and expressions of regulatory proinflammatory factors in the rat meninx under lipopolysaccharide treatment in vivo and in vitro  Histol Histopathol  2009  24(1):13-24  PubMed ID: 19012240   DOI: 10.14670/HH-24.13

2782  Tsujino K, Yamate J, Tsukamoto Y, Kumagai D, Kannan Y, Jippo T, Kuwamura M, Kotani T, Takeya M, Sakuma S  Establishment and characterization of cell lines derived from a transplantable rat malignant meningioma: morphological heterogeneity and production of nerve growth factor.  Acta Neuropathol  1997  93:461-70  PubMed ID: 9144584   DOI: 10.1007/s004010050640

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