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Cell No. : Cell Name
RCB1752 : BS-SHI-4M  update : 2022/04/07
CommentHuman cell line derived from Bloom syndrome patient. Transformed by EBV. Can express certain antigen on the cell surface, such as cancer-specific antigen.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Aoyama, Muneo
Originator Shiraishi, Yukimasa
Year of deposit 2001
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue peripheral blood
Disease name Bloom Syndrome
Classification transformed
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_A798
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 45-47(50) : 45(11),46(36),47(3)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
3296  Shiraishi Y, Ogata T, Soma H  Enhanced expression of stomach cancer antigen derived from malignantly transformed bloom syndrome cells previously labeled with bromodeoxyuridine.  Cancer Genet Cytogenet  1992  62:1-8  PubMed ID: 1521223   DOI: 10.1016/0165-4608(92)90028-7
3295  Shiraishi Y, Soma H  Cancer antigens are expressed in a carcinogen-transformed Bloom syndrome B-lymphoblastoid cell line.  Proc Natl Acad Sci U S A  1988  85:8211-5  PubMed ID: 3186719   DOI: 10.1073/pnas.85.21.8211

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