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Cell No. : Cell Name
RCB1750 : ΔRad18-DT40  update : 2023/01/04
CommentSubline of DT40 cell line, lacking Rad18 expression.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takeda, Shunichi
Originator Takata, Minoru
Year of deposit 2001
Original cell DT40
Animal _avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene pKO-Rad18-his, pKO-Rad18-hyg
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1U31
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 10-20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information Reference 5
User's Publication 0

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17859  Zhao Z, Wu X, He F, Xiang C, Feng X, Bai X, Liu X, Zhao J, Takeda S, Qing Y.  Critical roles of Rad54 in tolerance to apigenin-induced Top1-mediated DNA damage  Exp Ther Med  2021  21(5):505  PubMed ID: 33791014   DOI: 10.3892/etm.2021.9936
17845  Sharma V, Collins LB, Chen TH, Herr N, Takeda S, Sun W, Swenberg JA, Nakamura J.  Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations  Oncotarget  2016  7(18):25377-90  PubMed ID: 27015367   DOI: 10.18632/oncotarget.8298
3435  Mizutani A, Okada T, Shibutani S, Sonoda E, Hochegger H, Nishigori C, Miyachi Y, Takeda S, Yamazoe M.  Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells.  Cancer Res.   2004  64(9):3144-7  PubMed ID: 15126352   DOI: 10.1158/0008-5472.can-03-3489
3441  Okada T, Sonoda E, Yamashita YM, Koyoshi S, Tateishi S, Yamaizumi M, Takata M, Ogawa O, Takeda S  Involvement of vertebrate polkappa in Rad18-independent postreplication repair of UV damage  J Biol Chem  2002  277:48690-5  PubMed ID: 12356753   DOI: 10.1074/jbc.M207957200
3442  Yamashita YM, Okada T, Matsusaka T, Sonoda E, Zhao GY, Araki K, Tateishi S, Yamaizumi M, Takeda S  RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells.  EMBO J  2002  21:5558-66  PubMed ID: 12374756   DOI: 10.1093/emboj/cdf534

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