Cell No. : Cell Name
RCB1750 : ΔRad18-DT40
update : 2024/01/17
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| Comment | Subline of DT40 cell line, lacking Rad18 expression. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takeda, Shunichi
|
| Originator |
Takata, Minoru
|
| Year of deposit |
2001
|
| Original cell |
DT40
|
| Animal |
_avian
< Birds
|
| Tissue |
B cell
|
| Classification |
mutant
|
| Recombinant |
recombinant
|
| Exogene |
pKO-Rad18-his, pKO-Rad18-hyg
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_1U31
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage cell No |
|
1.5-2 x 10 5 cells/ml
|
| Passage ratio |
|
1 : 10-20 split
|
| SC frequency |
|
Subculture : every 2 days
|
| Temperature |
|
39.5
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Others |
|
Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
|
| Reference information |
Reference |
5
|
| User's Publication |
0
|
| Reference |
|
17859
Zhao Z, Wu X, He F, Xiang C, Feng X, Bai X, Liu X, Zhao J, Takeda S, Qing Y.
Critical roles of Rad54 in tolerance to apigenin-induced Top1-mediated DNA damage
Exp Ther Med
2021
21(5):505
PubMed ID: 33791014
DOI: 10.3892/etm.2021.9936
|
|
17845
Sharma V, Collins LB, Chen TH, Herr N, Takeda S, Sun W, Swenberg JA, Nakamura J.
Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations
Oncotarget
2016
7(18):25377-90
PubMed ID: 27015367
DOI: 10.18632/oncotarget.8298
|
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3435
Mizutani A, Okada T, Shibutani S, Sonoda E, Hochegger H, Nishigori C, Miyachi Y, Takeda S, Yamazoe M.
Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells.
Cancer Res.
2004
64(9):3144-7
PubMed ID: 15126352
DOI: 10.1158/0008-5472.can-03-3489
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3441
Okada T, Sonoda E, Yamashita YM, Koyoshi S, Tateishi S, Yamaizumi M, Takata M, Ogawa O, Takeda S
Involvement of vertebrate polkappa in Rad18-independent postreplication repair of UV damage
J Biol Chem
2002
277:48690-5
PubMed ID: 12356753
DOI: 10.1074/jbc.M207957200
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3442
Yamashita YM, Okada T, Matsusaka T, Sonoda E, Zhao GY, Araki K, Tateishi S, Yamaizumi M, Takeda S
RAD18 and RAD54 cooperatively contribute to maintenance of genomic stability in vertebrate cells.
EMBO J
2002
21:5558-66
PubMed ID: 12374756
DOI: 10.1093/emboj/cdf534
|
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