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Cell No. : Cell Name
RCB1747 : ΔNBS1-DT40  update : 2023/01/05
CommentSubline of DT40 (chicken B cell line), lacking Nbs1 expression.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Takeda, Shunichi
Originator Tauchi, Hiroshi
Year of deposit 2001
Original cell DT40
Animal _avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene pKO-Nbs1-hyr, pKO-Nbs1-bsr, pKO-Nbs1-puro
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T33
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 10-20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information Reference 2
User's Publication 0

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17830  Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki MT, Pommier Y, Takeda S, Sasanuma H.  Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines  Genes Cells  2015  20(12):1059-76  PubMed ID: 26525166   DOI: 10.1111/gtc.12310
3124  Tauchi, Hiroshi, Kobayashi, Junya, Morishima, Ken-ichi, van Gent, Dik C, Shiraishi, Takahiro, Verkaik, Nicole S, vanHeems, Diana, Ito, Emi, Nakamura, Asako, Sonoda, Eiichiro, Takata, Minoru, Takeda, Shunichi, Matsuura, Shinya, Komatsu, Kenshi  Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells.  Nature  2002  420:93-8  PubMed ID: 12422221   DOI: 10.1038/nature01125

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