Cell No. : Cell Name RCB1747 : ΔNBS1-DT40  update : 2024/01/17
Comment			Subline of DT40 (chicken B cell line), lacking Nbs1 expression.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Takeda, Shunichi
		Originator	Tauchi, Hiroshi
		Year of deposit	2001
		Original cell	DT40
		Animal	_avian < Birds
		Tissue	B cell
		Classification	mutant
		Recombinant	recombinant
		Exogene	pKO-Nbs1-hyr, pKO-Nbs1-bsr, pKO-Nbs1-puro
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_1T33
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture medium		RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
		Culture method
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage cell No		1.5-2 x 10 5 cells/ml
		Passage ratio		1 : 10-20 split
		SC frequency		Subculture : every 2 days
		Temperature		39.5 ℃
		CO2 concentration		5 %
		Freeze medium		90% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Others		Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information		Reference	2
		User's Publication	0

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Reference

17830  Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki MT, Pommier Y, Takeda S, Sasanuma H.  Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines  Genes Cells  2015  20(12):1059-76  PubMed ID: 26525166   DOI: 10.1111/gtc.12310

3124  Tauchi, Hiroshi, Kobayashi, Junya, Morishima, Ken-ichi, van Gent, Dik C, Shiraishi, Takahiro, Verkaik, Nicole S, vanHeems, Diana, Ito, Emi, Nakamura, Asako, Sonoda, Eiichiro, Takata, Minoru, Takeda, Shunichi, Matsuura, Shinya, Komatsu, Kenshi  Nbs1 is essential for DNA repair by homologous recombination in higher vertebrate cells.  Nature  2002  420:93-8  PubMed ID: 12422221   DOI: 10.1038/nature01125

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