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Cell No. : Cell Name
RCB1741 : NHOS  update : 2023/01/05
CommentMouse osteosarcoma cell line. Transplantable to mouse and form bone tissue in mouse.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kusumi, Tomomi
Originator Nishi, Takashi
Year of deposit 2001
Animal _mouse < Mammals
Strain name BALB/c
Gender Female
Tissue soft tissue tumor
Metastastatic ability Yes
Metastatsd tissue lung (a mouse/100 mice)
Classification cancer
Year of origin 1994
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6688
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 4 x 10 6 cells/60 mm dish
Passage ratio 1 : 8 split
SC frequency Subculture : 3-4 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 2

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4985  Nishi T, Kusumi T, Tanaka M, Sato F, Sasaki M, Kudo H, Kijima H.  Establishment of transplantable murine osteosarcoma cell line with endochondral ossification.  Anticancer Res  2008  28(3A):1627-31  PubMed ID: 18630519  

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User's Publication
20656  Ogiwara Y, Nakagawa M, Nakatani F, Uemura Y, Zhang R, Kudo-Saito C.  Blocking FSTL1 boosts NK immunity in treatment of osteosarcoma.  Cancer Lett  2022  537:215690  PubMed ID: 35439537   DOI: 10.1016/j.canlet.2022.215690
2557  Kusumi T, Nishi T, Tanaka M, Tsuchida S, Kudo H  A murine osteosarcoma cell line with a potential to develop ossification upon transplantation  Jpn J Cancer Res  2001  92:649-658  PubMed ID: 11429054   DOI: 10.1111/j.1349-7006.2001.tb01144.x

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