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Cell No. : Cell Name
RCB1741 : NHOS update : 2023/01/05 |
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| Comment | Mouse osteosarcoma cell line. Transplantable to mouse and form bone tissue in mouse. | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Kusumi, Tomomi | ||
| Originator | Nishi, Takashi | |||
| Year of deposit | 2001 | |||
| Animal | _mouse < Mammals | |||
| Strain name | BALB/c | |||
| Gender | Female | |||
| Tissue | soft tissue tumor | |||
| Metastatic ability | Yes | |||
| Metastatic tissue | lung (a mouse/100 mice) | |||
| Classification | cancer | |||
| Year of origin | 1994 | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_6688 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | RPMI1640 + 10% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage cell No | 4 x 10 6 cells/60 mm dish |
| Passage ratio | 1 : 8 split | |||
| SC frequency | Subculture : 3-4 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| SSLP(mouse) | OK | |||
| Isozyme | LD, NP | |||
| Reference information | Reference | 1 | ||
| User's Publication | 2 | |||
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