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Cell No. : Cell Name
RCB1734 : LAH3  update : 2023/01/06
CommentMelanophore derived cell line. Derived from Rana porosa brevipoda (albino).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Okumoto, Hitoshi
Originator Okumoto, Hitoshi
Year of deposit 2001
Animal _frog < Amphibians
Genus Rana
Species porpsa brevipoda
Tissue skin
Disease name melanophore
Classification other
Year of origin 1985
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_6668
deposit info
lot info
Medium Medium List
Culture medium 60% L-15 + 30% D.D.W. + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/3-4 weeks, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
deposit info
lot info
Reference information Reference 2
User's Publication 0

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2780  Okumoto Hitoshi  Establishment of three cell lines derived from frog ,elanophores   Zoological Science  2001  18:483-496    DOI: 10.2108/zsj.18.483
2781  Okumoto H, Nishioka M, Miura I, Obika M  Melanosome formation in cultured amelanotic melanophores of Rana brevipoda by a frog tyrosinase cDNA Transfection.  Pigment Cell Res  1995  8:187-93  PubMed ID: 8610069   DOI: 10.1111/j.1600-0749.1995.tb00662.x

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