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Cell No. : Cell Name
RCB1728 : Hpr-4  update : 2023/01/05
CommentH2O2 resistant Chinese Hamster V79 cells
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kaneko, Motohisa
Originator Kaneko, Motohisa
Year of deposit 2001
Original cell V79
Animal _hamster < Mammals
Strain name Chinese
Gender Male
Tissue lung
Classification transformed
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4706
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 6
User's Publication 0


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Reference
2796  Kaneko M, Inoue F, Oda T  Calcium associated resistance to H(2)O(2) in Chinese hamster V79 cells.  Toxicol Lett  2000  115:137-47  PubMed ID: 10802389   DOI: 10.1016/s0378-4274(00)00184-3
2798  Kaneko M, Inoue F  The sensitivity to DNA single strand breakage in mitochondria, but not in nuclei, of Chinese hamster V79 and variant cells correlates with their cellular sensitivity to hydrogen peroxide.  Toxicol Lett  1998  99:15-22  PubMed ID: 9801026   DOI: 10.1016/s0378-4274(98)00132-5
2797  Kaneko M, Kodama M, Inoue F, Terasaki T  Mitochondrial biosynthesis controls the sensitivity of Chinese hamster cells to hydrogen peroxide.  Free Radic Res  1996  24:299-309  PubMed ID: 8731014   DOI: 10.3109/10715769609088027
2799  Kaneko M, Kodama M, Inoue F  Bimodal pattern of killing of Chinese hamster V79 variant cells by hydrogen peroxide.  Free Radic Res  1994  20:229-39  PubMed ID: 8205225   DOI: 10.3109/10715769409147519
346  ELKIND, M M, SUTTON H  Radiation response of mammalian cells grown in culture. 1. Repair of X-ray damage in surviving Chinese hamster cells.  Radiat Res  1960  13:556-93  PubMed ID: 13726391  
344  FORD, D K, YERGANIAN G  Observations on the chromosomes of Chinese hamster cells in tissue culture.  J Natl Cancer Inst  1958  21:393-425  PubMed ID: 13576097  

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