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Cell No. : Cell Name
RCB1709 : GE1  update : 2022/05/30
CommentMouse-derived gingival epithelial cell line. Derived from SV40-Large T antigen transgenic mouse. Application consideration
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref.2811(J Oral Pathol Med 2001;30:296-304) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hatakeyama, Setsuko
Originator Hatakeyama, Setsuko
Year of deposit 2000
Animal mouse < Mammals
Strain name C57BL/6
Gender Female
Age at sampling 2.5 months
Tissue gingival kerarinocyte
Classification transformed
Recombinant recombinant
Exogene pSVtsA58
Year of origin 1996
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E112
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives SFM-101 + 1% FBS + 10ng/ml mouse EGF
Antibiotics Free
Passage method (0.24% Trypsin + 0.08% EDTA) or (0.25% Trypsin + 0.04% EDTA), washed twice with medium
Culture information Passage cell No 4 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 22


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Reference
8104  Hatakeyama S, Mizusawa N, Tsutsumi R, Yoshimoto K, Mizuki H, Yasumoto S, Sato S, Takeda Y.  Establishment of human dental epithelial cell lines expressing ameloblastin and enamelin by transfection of hTERT and cdk4 cDNAs.  J. Oral Pathol. Med.  2011  40:227-34  PubMed ID: 20923449   DOI: 10.1111/j.1600-0714.2010.00950.x
2811  Hatakeyama S, Ohara-Nemoto Y, Yanai N, Obinata M, Hayashi S, Satoh M  Establishment of gingival epithelial cell lines from transgenic mice harboring temperature sensitive simian virus 40 large T-antigen gene.  J Oral Pathol Med  2001  30:296-304  PubMed ID: 11334466   DOI: 10.1034/j.1600-0714.2001.300507.x

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User's Publication
20577  Lu HY, Hou J, Takahashi Y, Tamura Y, Kasugai S, Kuroda S, Nakata H.  Periodontal Pathogen Adhesion, Cytotoxicity, and Surface Free Energy of Different Materials for an Implant Prosthesis Screw Access Hole.  Medicina (Kaunas)  2022  58(2)  PubMed ID: 35208651   DOI: 10.3390/medicina58020329
20268  Arkin VH, Narendrakumar U, Madhyastha H, Manjubala I.  Characterization and In Vitro Evaluations of Injectable Calcium Phosphate Cement Doped with Magnesium and Strontium.  ACS Omega  2021  6(4):2477-2486  PubMed ID: 33553866   DOI: 10.1021/acsomega.0c03927
12008  Nakayama Y, Inoue E, Kato A, Iwai Y, Takai-Yamazaki M, Tsuruya Y, Yamaguchi A, Noda K, Nomoto T, Ganss B, Ogata Y.  Follicular dendritic cell-secreted protein gene expression is upregulated and spread in nifedipine-induced gingival overgrowth.  Odontology  2020    PubMed ID: 31955298   DOI: 10.1007/s10266-020-00483-2
13121  Endo K, Mine Y, Shuto T, Taji T, Murayama T, Nikawa H.  Comprehensive analysis of transcriptional profiles in oral epithelial-like cells stimulated with oral probiotic Lactobacillus spp.  Arch Oral Biol  2020    PubMed ID: 32739629   DOI: 10.1016/j.archoralbio.2020.104832
13165  Haraguchi T, Ayukawa Y, Shibata Y, Takeshita T, Atsuta I, Ogino Y, Yasunami N, Yamashita Y, Koyano K.  Effect of Calcium Chloride Hydrothermal Treatment of Titanium on Protein, Cellular, and Bacterial Adhesion Properties.  J Clin Med  2020    PubMed ID: 32823609   DOI: 10.3390/jcm9082627
11321  Nakayama Y, Tsuruya Y, Noda K, Yamazaki-Takai M, Iwai Y, Ganss B, Ogata Y.  Negative feedback by SNAI2 regulates TGFβ1-induced amelotin gene transcription in epithelial-mesenchymal transition.  J. Cell. Physiol.  2019    PubMed ID: 30488439   DOI: 10.1002/jcp.27804
6926  Nakayama Y, Kobayashi R, Iwai Y, Noda K, Yamazaki M, Kurita-Ochiai T, Yoshimura A, Ganss B, Ogata Y.  C/EBPβ and YY1 bind and interact with Smad3 to modulate lipopolysaccharide-induced amelotin gene transcription in mouse gingival epithelial cells.  FEBS Open Bio.  2019  9(2):276-290  PubMed ID: 30761253   DOI: 10.1002/2211-5463.12566
6955  Imai M, Ayukawa Y, Yasunami N, Furuhashi A, Takemura Y, Adachi N, Hu J, Zhou X, Moriyama Y, Atsuta I, Kurata K, Koyano K.  Effect of a Single Injection of Benidipine-Impregnated Biodegradable Microcarriers on Bone and Gingival Healing at the Tooth Extraction Socket.  Adv Wound Care (New Rochelle)  2019  8(3):108-117  PubMed ID: 30911442   DOI: 10.1089/wound.2018.0834
6959  Sakamoto Y, Ayukawa Y, Furuhashi A, Kamo M, Ikeda J, Atsuta I, Haraguchi T, Koyano K.  Effect of Hydrothermal Treatment with Distilled Water on Titanium Alloy for Epithelial Cellular Attachment.  Materials (Basel).  2019  12(17):2748  PubMed ID: 31461930   DOI: 10.3390/ma12172748
10818  Nakayama Y, Matsui S, Noda K, Yamazaki M, Iwai Y, Ganss B, Ogata Y.  TGFβ1-induced Amelotin gene expression is downregulated by Bax expression in mouse gingival epithelial cells.  J Oral Sci  2018  60(2):232-241  PubMed ID: 29657250   DOI: 10.2334/josnusd.17-0271
11191  Noda K, Yamazaki M, Iwai Y, Matsui S, Kato A, Takai H, Nakayama Y, Ogata Y.  IL-1β and TNF-α regulate mouse amelotin gene transcription in gingival epithelial cells.  J Oral Sci  2018  60(3):388-398  PubMed ID: 30158339   DOI: 10.2334/josnusd.17-0388
9628  Iwanabe Y, Masaki C, Tamura A, Tsuka S, Mukaibo T, Kondo Y, Hosokawa R.  The effect of low-intensity pulsed ultrasound on wound healing using scratch assay in epithelial cells.  J Prosthodont Res  2016  60:308-314  PubMed ID: 27026212   DOI: 10.1016/j.jpor.2016.03.002
9777  Nakayama Y, Matsui S, Noda K, Yamazaki M, Iwai Y, Matsumura H, Izawa T, Tanaka E, Ganss B, Ogata Y.  Amelotin gene expression is temporarily being upregulated at the initiation of apoptosis induced by TGFβ1 in mouse gingival epithelial cells.  Apoptosis  2016  21:1057-70  PubMed ID: 27502207   DOI: 10.1007/s10495-016-1279-5
7446  Kuramitsu-Fujimoto S, Ariyoshi W, Saito N, Okinaga T, Kamo M, Ishisaki A, Takata T, Yamaguchi K, Nishihara T.  Novel biological activity of ameloblastin in enamel matrix derivative.  J Appl Oral Sci  2015  23:49-55  PubMed ID: 25760267   DOI: 10.1590/1678-775720140291
9393  Terukazu Sanui, Urara Tanaka, Takao Fukuda, Kyosuke Toyoda, Takaharu Taketomi, Ryo Atomura, Kensuke Yamamichi, Fusanori Nishimura  Mutation of Spry2 Induces Proliferation and Differentiation of Osteoblasts but Inhibits Proliferation of Gingival Epithelial Cells  J Cell Biochem  2015  116(4):628-39  PubMed ID: 25399781   DOI: 10.1002/jcb.25014
9398  Toru Fukuda 1, Hiroki Ochi 1, Satoko Sunamura 1, Akina Haide  MicroRNA-145 Regulates Osteoblastic Differentiation by Targeting the Transcription Factor Cbfb  FEBS Lett  2015  589(21):3302-8  PubMed ID: 26450370   DOI: 10.1016/j.febslet.2015.09.024
9406  Noriyuki Yasunami, Yasunori Ayukawa, Akihiro Furuhashi, Ikiru Atsuta, Yunia Dwi Rakhmatia, Yasuko Moriyama, Tomohiro Masuzaki, Kiyoshi Koyano  Acceleration of Hard and Soft Tissue Healing in the Oral Cavity by a Single Transmucosal Injection of Fluvastatin-Impregnated Poly (Lactic-Co-Glycolic Acid) Microspheres. An in Vitro and Rodent in Vivo Study  Biomed Mater  2015  11(1):015001  PubMed ID: 26694986   DOI: 10.1088/1748-6041/11/1/015001
16350  Miyata K, Takebe J.  Anodized-hydrothermally treated titanium with a nanotopographic surface structure regulates integrin-α6β4 and laminin-5 gene expression in adherent murine gingival epithelial cells  J Prosthodont Res  2013  57(2):99-108  PubMed ID: 23415882   DOI: 10.1016/j.jpor.2012.12.002
17189  Kawabata S, Asano K, Miyazawa A, Satoh T, Tabata Y.  Electrodeposition of pronectin for titanium to augment gingival epithelium adhesion  J Tissue Eng Regen Med  2013  7(5):348-52  PubMed ID: 22294437   DOI: 10.1002/term.527
6096  Sakamoto K, Fujii T, Kawachi H, Miki Y, Omura K, Morita K, Kayamori K, Katsube K, Yamaguchi A.  Reduction of NOTCH1 expression pertains to maturation abnormalities of keratinocytes in squamous neoplasms.  Lab Invest  2012  92(5):688-702  PubMed ID: 22330335   DOI: 10.1038/labinvest.2012.9
8401  Akihiro Toshinaga, Ryuji Hosokawa, Toshinori Okinaga, Chihiro Masaki, Toshiyuki Tsujisawa, Tatsuji Nishihara  Inflammatory response in epithelial cells induced by mechanical stress is suppressed by hyaluronic acid  Inflammation and Regeneration  2010  30:120-127    DOI: 10.2492/inflammregen.30.120
8265  Mine Y, Makihira S, Nikawa H, Murata H, Hosokawa R, Hiyama A, Mimura S.  Impact of titanium ions on osteoblast-, osteoclast- and gingival epithelial-like cells.  J Prosthodont Res  2010  54(1):1-6  PubMed ID: 19733525   DOI: 10.1016/j.jpor.2009.07.003



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