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Cell No. : Cell Name
RCB1709 : GE1  update : 2018/08/01
CommentMouse-derived gingival epithelial cell line. Derived from SV40-Large T antigen transgenic mouse. Application consideration
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref.2811(J Oral Pathol Med 2001;30:296-304) designated by the DEPOSITOR is required.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Hatakeyama, Setsuko
Originator Hatakeyama, Setsuko
Year of deposit 2000
Animal mouse < Mammals
Strain name C57BL/6
Gender Female
Age at sampling 2.5 months
Tissue gingival kerarinocyte
Classification transformed
Recombinant recombinant
Exogene pSVtsA58
Year of origin 1996
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives SFM-101 + 1% FBS + 10ng/ml mouse EGF
Antibiotics Free
Passage method (0.24% trypsin + 0.08% EDTA) or (0.25% trypsin + 0.02% EDTA), washed twice with medium
Culture information Passage cell No 4 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 17

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2811  Hatakeyama S, Ohara-Nemoto Y, Yanai N, Obinata M, Hayashi S, Satoh M  Establishment of gingival epithelial cell lines from transgenic mice harboring temperature sensitive simian virus 40 large T-antigen gene.  J Oral Pathol Med  2001  30:296-304  PubMed ID: 11334466

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User's Publication
12008  Nakayama Y, Inoue E, Kato A, Iwai Y, Takai-Yamazaki M, Tsuruya Y, Yamaguchi A, Noda K, Nomoto T, Ganss B, Ogata Y.  Follicular dendritic cell-secreted protein gene expression is upregulated and spread in nifedipine-induced gingival overgrowth.  Odontology  2020    PubMed ID: 31955298
11321  Nakayama Y, Tsuruya Y, Noda K, Yamazaki-Takai M, Iwai Y, Ganss B, Ogata Y.  Negative feedback by SNAI2 regulates TGFβ1-induced amelotin gene transcription in epithelial-mesenchymal transition.  J. Cell. Physiol.  2019    PubMed ID: 30488439
6955  Imai M, Ayukawa Y, Yasunami N, Furuhashi A, Takemura Y, Adachi N, Hu J, Zhou X, Moriyama Y, Atsuta I, Kurata K, Koyano K.  Effect of a Single Injection of Benidipine-Impregnated Biodegradable Microcarriers on Bone and Gingival Healing at the Tooth Extraction Socket.  Adv Wound Care (New Rochelle)  2019  8(3):108-117  PubMed ID: 30911442
6959  Sakamoto Y, Ayukawa Y, Furuhashi A, Kamo M, Ikeda J, Atsuta I, Haraguchi T, Koyano K.  Effect of Hydrothermal Treatment with Distilled Water on Titanium Alloy for Epithelial Cellular Attachment.  Materials (Basel).  2019  12(17):2748  PubMed ID: 31461930
10818  Nakayama Y, Matsui S, Noda K, Yamazaki M, Iwai Y, Ganss B, Ogata Y.  TGFβ1-induced Amelotin gene expression is downregulated by Bax expression in mouse gingival epithelial cells.  J Oral Sci  2018  60(2):232-241  PubMed ID: 29657250
11191  Noda K, Yamazaki M, Iwai Y, Matsui S, Kato A, Takai H, Nakayama Y, Ogata Y.  IL-1β and TNF-α regulate mouse amelotin gene transcription in gingival epithelial cells.  J Oral Sci  2018  60(3):388-398  PubMed ID: 30158339
6926  Nakayama Y, Kobayashi R, Iwai Y, Noda K, Yamazaki M, Kurita-Ochiai T, Yoshimura A, Ganss B, Ogata Y.  C/EBPβ and YY1 bind and interact with Smad3 to modulate lipopolysaccharide-induced amelotin gene transcription in mouse gingival epithelial cells.  FEBS Open Bio.  2018  9(2):276-290  PubMed ID: 30761253
9628  Iwanabe Y, Masaki C, Tamura A, Tsuka S, Mukaibo T, Kondo Y, Hosokawa R.  The effect of low-intensity pulsed ultrasound on wound healing using scratch assay in epithelial cells.  J Prosthodont Res  2016  60:308-314  PubMed ID: 27026212
9777  Nakayama Y, Matsui S, Noda K, Yamazaki M, Iwai Y, Matsumura H, Izawa T, Tanaka E, Ganss B, Ogata Y.  Amelotin gene expression is temporarily being upregulated at the initiation of apoptosis induced by TGFβ1 in mouse gingival epithelial cells.  Apoptosis  2016  21:1057-70  PubMed ID: 27502207
7446  Kuramitsu-Fujimoto S, Ariyoshi W, Saito N, Okinaga T, Kamo M, Ishisaki A, Takata T, Yamaguchi K, Nishihara T.  Novel biological activity of ameloblastin in enamel matrix derivative.  J Appl Oral Sci  2015  23:49-55  PubMed ID: 25760267
9393  Terukazu Sanui, Urara Tanaka, Takao Fukuda, Kyosuke Toyoda, Takaharu Taketomi, Ryo Atomura, Kensuke Yamamichi, Fusanori Nishimura  Mutation of Spry2 Induces Proliferation and Differentiation of Osteoblasts but Inhibits Proliferation of Gingival Epithelial Cells  J Cell Biochem  2015  116(4):628-39  PubMed ID: 25399781
9398  Toru Fukuda 1, Hiroki Ochi 1, Satoko Sunamura 1, Akina Haide  MicroRNA-145 Regulates Osteoblastic Differentiation by Targeting the Transcription Factor Cbfb  FEBS Lett  2015  589(21):3302-8  PubMed ID: 26450370
9406  Noriyuki Yasunami, Yasunori Ayukawa, Akihiro Furuhashi, Ikiru Atsuta, Yunia Dwi Rakhmatia, Yasuko Moriyama, Tomohiro Masuzaki, Kiyoshi Koyano  Acceleration of Hard and Soft Tissue Healing in the Oral Cavity by a Single Transmucosal Injection of Fluvastatin-Impregnated Poly (Lactic-Co-Glycolic Acid) Microspheres. An in Vitro and Rodent in Vivo Study  Biomed Mater  2015  11(1):015001  PubMed ID: 26694986
6096  Sakamoto K, Fujii T, Kawachi H, Miki Y, Omura K, Morita K, Kayamori K, Katsube K, Yamaguchi A.  Reduction of NOTCH1 expression pertains to maturation abnormalities of keratinocytes in squamous neoplasms.  Lab Invest  2012  92(5):688-702  PubMed ID: 22330335
8104  Hatakeyama S, Mizusawa N, Tsutsumi R, Yoshimoto K, Mizuki H, Yasumoto S, Sato S, Takeda Y.  Establishment of human dental epithelial cell lines expressing ameloblastin and enamelin by transfection of hTERT and cdk4 cDNAs.  J. Oral Pathol. Med.  2011  40:227-34  PubMed ID: 20923449
8401  Akihiro Toshinaga, Ryuji Hosokawa, Toshinori Okinaga, Chihiro Masaki, Toshiyuki Tsujisawa, Tatsuji Nishihara  Inflammatory response in epithelial cells induced by mechanical stress is suppressed by hyaluronic acid  Inflammation and Regeneration  2010  30:120-127 
8265  Mine Y, Makihira S, Nikawa H, Murata H, Hosokawa R, Hiyama A, Mimura S.  Impact of titanium ions on osteoblast-, osteoclast- and gingival epithelial-like cells.  J Prosthodont Res  2010  54(1):1-6  PubMed ID: 19733525

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