Cell No. : Cell Name
update : 2023/01/04
Comment Human cell line derived from metastasis at ovary of colon cancer. Moderately differentiated adenocarcinoma. Application consideration
Comment from the depositor
Terms and conditions A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
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English
Address
Contact Us
Order Form
Order Form(C-0005.pdf)
Approval Form(C-0006.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see ). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp ).
Basic information
Depositor
Ishikawa, Hiroshi
Originator
Ishikawa, H. & Yasuda, M.
Year of deposit
2000
Animal
_human
< Mammals
Genus
Homo
Species
sapiens
Race
Japanese
Gender
Female
Age at sampling
52 years
Tissue
colon, ovary meta
Disease name
Moderately differentiated adenocarcinoma
Metastatic ability
Yes
Metastatic tissue
ovary
Classification
cancer
Year of origin
2000
Lifespan
infinite
Morphology
epithelial-like
Cellosaurus(Expasy)
CVCL_E075
deposit info lot info
Medium
Medium List
Culture type
Adherent cells
Culture medium
DMEM/HamF12 + 10% FBS + 0.1mM NEAA
Antibiotics
Free
Passage method
(0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information
Passage ratio
1 : 4 split
SC frequency
Subculture : once/5-6 weeks, Medium Renewal : 2 times/week
Temperature
37
℃
CO2 concentration
5
%
Freeze medium
Medium + 10% DMSO
Freezing method
Slow freezing
Mycoplasma/Acholeplasma
(-)
Isozyme
LD, NP
STR(human)
OK
Reference information
Reference
0
User's Publication
0
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