Cell No. : Cell Name
RCB1704 : BLNK^(-)/Syk^(-) DT40
update : 2024/01/17
|
| Comment | BLNK and Syk deficient DT40 cells |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kurosaki, Tomohiro
|
| Originator |
Kurosaki, Tomohiro
|
| Year of deposit |
2000
|
| Another name |
E37-5
|
| Original cell |
DT40
|
| Animal |
_avian
< Birds
|
| Tissue |
B cell
|
| Classification |
mutant
|
| Recombinant |
recombinant
|
| Exogene |
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_1T52
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage cell No |
|
1.5-2 x 10 5 cells/ml
|
| Passage ratio |
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1 : 20 split
|
| SC frequency |
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Subculture : every 2 days
|
| Temperature |
|
39.5
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Others |
|
Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
| Reference |
|
2728
Ishiai M, Kurosaki M, Inabe K, Chan AC, Sugamura K, Kurosaki T
Involvement of LAT, Gads, and Grb2 in compartmentation of SLP-76 to the plasma membrane.
J Exp Med
2000
192:847-56
PubMed ID: 10993915
DOI: 10.1084/jem.192.6.847
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|
2284
Takata M, Sabe H, Hata A, Inazu T, Homma Y, Nukada T, Yamamura H, Kurosaki T.
Tyrosine kinases Lyn and Syk regulate B cell receptor-coupled Ca2+ mobilization through distinct pathways.
EMBO J
1994
13(6):1341-9
PubMed ID: 8137818
DOI: 10.1002/j.1460-2075.1994.tb06387.x
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