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Cell No. : Cell Name
RCB1701 : MY  update : 2024/09/10
CommentHuman cell line derived from p180-BCR-ABL dependent leukemia.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Inokuchi, Koiti
Originator Inokuchi, Koiti
Year of deposit 2000
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 52 years
Tissue peripheral blood lymphocytes
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_3034
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 46-48(50) : 46(10),47(36),48(4)
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
3138  Inokuchi K, Shinohara T, Futaki M, Hanawa H, Tanosaki S, Yamaguchi H, Nomura T, Dan K.  Establishment of a cell line with variant BCR/ABL breakpoint expressing P180BCR/ABL from late-appearing Philadelphia-positive acute biphenotypic leukemia.  Genes Chromosomes Cancer  1998  23(3):227-38  PubMed ID: 9790503   DOI: 10.1002/(sici)1098-2264(199811)23:3<227::aid-gcc4>3.0.co;2-3

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