Back Back
Cell No. : Cell Name
RCB1699 : LY-B/cLCB1  update : 2022/04/06
CommentLY-B derivative complemented the Serine-palmitoyl transferase (LCB1 subunit) deficiency.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 1998;273:33787-33794) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hanada, Kentaro
Originator Hanada, Kentaro
Year of deposit 2000
Original cell CHO-K1
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Recombinant recombinant
Exogene pSV-OKneo, LCB1 gene cDNA
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U862
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% NBS (or FBS)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 1-5 x 10^(5) cell/100 mm dish
Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
Reference information Reference 2
User's Publication 1

To topTop
2687  Hanada K, Hara T, Fukasawa M, Yamaji A, Umeda M, Nishijima M  Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin. Genetic and biochemical evidence for complex formation of the LCB1 protein with the LCB2 protein for serine palmitoyltransferase.  J Biol Chem  1998  273:33787-94  PubMed ID: 9837968  
2686  Hanada K, Hara T, Nishijima M, Kuge O, Dickson, R C, Nagiec, M M  A mammalian homolog of the yeast LCB1 encodes a component of serine palmitoyltransferase, the enzyme catalyzing the first step in sphingolipid synthesis.  J Biol Chem  1997  272:32108-14  PubMed ID: 9405408  

To topTop
User's Publication
8301  Eshraghi A, Maldonado-Arocho FJ, Gargi A, Cardwell MM, Prouty MG, Blanke SR, Bradley KA.  Cytolethal distending toxin family members are differentially affected by alterations in host glycans and membrane cholesterol.  J. Biol. Chem.  2010  285:18199-207  PubMed ID: 20385557   DOI: 10.1074/jbc.M110.112912

Back Back Return Top Page