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Cell No. : Cell Name
RCB1698 : LY-B  update : 2023/09/21
CommentSerine-palmitoyl transferase (LCB1 subunit) deficient cells derived from CHO-K1 cell line.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 1998;273:33787-33794) designated by the DEPOSITOR is required.
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Basic information Depositor Hanada, Kentaro
Originator Hanada, Kentaro
Year of deposit 2000
Original cell CHO-K1
Animal _hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4701
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% NBS (or FBS)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 1-5 x 10 5 cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
Reference information Reference 2
User's Publication 4

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2687  Hanada K, Hara T, Fukasawa M, Yamaji A, Umeda M, Nishijima M  Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin. Genetic and biochemical evidence for complex formation of the LCB1 protein with the LCB2 protein for serine palmitoyltransferase.  J Biol Chem  1998  273:33787-94  PubMed ID: 9837968   DOI: 10.1074/jbc.273.50.33787
2686  Hanada K, Hara T, Nishijima M, Kuge O, Dickson, R C, Nagiec, M M  A mammalian homolog of the yeast LCB1 encodes a component of serine palmitoyltransferase, the enzyme catalyzing the first step in sphingolipid synthesis.  J Biol Chem  1997  272:32108-14  PubMed ID: 9405408   DOI: 10.1074/jbc.272.51.32108

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User's Publication
20711  Monasterio BG, Jiménez-Rojo N, García-Arribas AB, Riezman H, Goñi FM, Alonso A.  Plasma membrane effects of sphingolipid-synthesis inhibition by myriocin in CHO cells: a biophysical and lipidomic study.  Sci Rep  2022  12(1):955  PubMed ID: 35046440   DOI: 10.1038/s41598-021-04648-z
20188  Monasterio BG, Jiménez-Rojo N, García-Arribas AB, Riezman H, Goñi FM, Alonso A.  CHO/LY-B cell growth under limiting sphingolipid supply: Correlation between lipid composition and biophysical properties of sphingolipid-restricted cell membranes.  FASEB J  2021  35(6):e21657  PubMed ID: 34010474   DOI: 10.1096/fj.202001879RR
14200  Romano JD, Sonda S, Bergbower E, Smith ME, Coppens I.  Toxoplasma gondii salvages sphingolipids from the host Golgi through the rerouting of selected Rab vesicles to the parasitophorous vacuole  Mol Biol Cell  2013  24(12):1974-95  PubMed ID: 23615442   DOI: 10.1091/mbc.E12-11-0827
8301  Eshraghi A, Maldonado-Arocho FJ, Gargi A, Cardwell MM, Prouty MG, Blanke SR, Bradley KA.  Cytolethal distending toxin family members are differentially affected by alterations in host glycans and membrane cholesterol.  J. Biol. Chem.  2010  285:18199-207  PubMed ID: 20385557   DOI: 10.1074/jbc.M110.112912

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