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Cell No. : Cell Name
RCB1697 : SPB-1/cLCB1  update : 2022/04/06
CommentSPB-1 derivative complemented the Serine-palmitoyl transferase deficiency.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 1990;265:22137-22142, J Biol Chem 1992;267:23527-23533) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hanada, Kentaro
Originator Hanada, Kentaro
Year of deposit 2000
Original cell CHO-K1
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Recombinant recombinant
Exogene pSV-OKneo, LCB1 gene cDNA
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T761
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% NBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 1-5 x 10^(5) cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
Images
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lot info
Reference information Reference 2
User's Publication 0


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Reference
2685  Hanada K, Nishijima M, Kiso M, Hasegawa A, Fujita S, Ogawa T, Akamatsu Y  Sphingolipids are essential for the growth of Chinese hamster ovary cells. Restoration of the growth of a mutant defective in sphingoid base biosynthesis by exogenous sphingolipids.  J Biol Chem  1992  267:23527-33  PubMed ID: 1429697  
2684  Hanada K, Nishijima M, Akamatsu Y  A temperature-sensitive mammalian cell mutant with thermolabile serine palmitoyltransferase for the sphingolipid biosynthesis.  J Biol Chem  1990  265:22137-42  PubMed ID: 2125045  

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