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Cell No. : Cell Name
RCB1697 : SPB-1/cLCB1  update : 2023/01/06
CommentSPB-1 derivative complemented the Serine-palmitoyl transferase deficiency.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 1990;265:22137-22142, J Biol Chem 1992;267:23527-23533) designated by the DEPOSITOR is required.
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Basic information Depositor Hanada, Kentaro
Originator Hanada, Kentaro
Year of deposit 2000
Original cell CHO-K1
Animal _hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Recombinant recombinant
Exogene pSV-OKneo, LCB1 gene cDNA
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T761
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Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% NBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 1-5 x 10 5 cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, MPI, PepB
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lot info
Reference information Reference 2
User's Publication 0

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2685  Hanada K, Nishijima M, Kiso M, Hasegawa A, Fujita S, Ogawa T, Akamatsu Y  Sphingolipids are essential for the growth of Chinese hamster ovary cells. Restoration of the growth of a mutant defective in sphingoid base biosynthesis by exogenous sphingolipids.  J Biol Chem  1992  267:23527-33  PubMed ID: 1429697  
2684  Hanada K, Nishijima M, Akamatsu Y  A temperature-sensitive mammalian cell mutant with thermolabile serine palmitoyltransferase for the sphingolipid biosynthesis.  J Biol Chem  1990  265:22137-42  PubMed ID: 2125045  

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