Cell No. : Cell Name
RCB1696 : SPB-1
update : 2023/01/06
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| Comment | Serine-palmitoyl transferase deficient (temperature-sensitive) cells derived from CHO-K1 cell line. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 1990;265:22137-22142, J Biol Chem 1992;267:23527-23533) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Hanada, Kentaro
|
| Originator |
Hanada, Kentaro
|
| Year of deposit |
2000
|
| Original cell |
CHO-K1
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
|
| Genus |
Cricetulus
|
| Species |
griseus
|
| Gender |
Female
|
| Age at sampling |
adult
|
| Tissue |
ovary
|
| Classification |
mutant
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_T760
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
|
HamF12 + 10% NBS
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage cell No |
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1-5 x 10 5 cells/100 mm dish
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| SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
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| Temperature |
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33
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
0
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