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Cell No. : Cell Name
RCB1692 : Tpit/E  update : 2022/05/30
CommentVascular endothelial cell-like cell line derived from an anterior pituitary gland of a transgenic mouse expressing temperature sensitive SV40 large T antigen. Producing endothelin.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Inoue, Kinji
Originator Inoue, Kinji
Year of deposit 2000
Animal mouse < Mammals
Strain name C57BL
Gender Unknown
Age at sampling 1 month
Tissue anterior pituitary gland
Classification semi-normal
Recombinant recombinant
Exogene large T
Year of origin 1995
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_U899
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
6664  Saishu Yoshida, Masashi Higuchi, Hiroki Ueharu, Naoto Nishimura, Mitsuyoshi Tsuda, Hideji Yako, Mo Chen, Hideo Mitsuishi, Yoshiya Sano, Takako Kato, Yukio Kato  Characterization of murine pituitary-derived cell lines Tpit/F1, Tpit/E and TtT/GF  J Reprod Dev  2014  60(4):295-303  PubMed ID: 24881870
2683  Chen L, Maruyama D, Sugiyama M, Sakai T, Mogi C, Kato M, Kurotani R, Shirasawa N, Takaki A, Renner U, Kato Y, Inoue K.  Cytological characterization of a pituitary folliculo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neuronal nitric oxide synthase expression and nitric oxide secretion  Endocrinology  2000  141:3603-3610  PubMed ID: 11014214

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