Cell No. : Cell Name
RCB1691 : Tpit/F1
update : 2023/01/06
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| Comment | Pituitary folliculo-stellate-like cell line. Derived from a transgenic mouse expressing temperature sensitive SV40 large T antigen. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Inoue, Kinji
|
| Originator |
Inoue, Kinji
|
| Year of deposit |
2000
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C57BL
|
| Gender |
Unknown
|
| Age at sampling |
1 month
|
| Tissue |
anterior pituitary gland
|
| Classification |
semi-normal
|
| Recombinant |
recombinant
|
| Exogene |
large T
|
| Year of origin |
1995
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_S513
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
33
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
1
|
| Reference |
|
6664
Saishu Yoshida, Masashi Higuchi, Hiroki Ueharu, Naoto Nishimura, Mitsuyoshi Tsuda, Hideji Yako, Mo Chen, Hideo Mitsuishi, Yoshiya Sano, Takako Kato, Yukio Kato
Characterization of murine pituitary-derived cell lines Tpit/F1, Tpit/E and TtT/GF
J Reprod Dev
2014
60(4):295-303
PubMed ID: 24881870
DOI: 10.1262/jrd.2014-031
|
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7535
Chihiro Mogi, Shunsuke Miyai, Yusuke Nishimura, Hitomi Fukuro, Kotaro Yokoyama, Atsushi Takaki, Kinji Inoue
Differentiation of skeletal muscle from pituitary folliculo-stellate cells and endocrine progenitor cells
Exp Cell Res
2004
292(2):288-94
PubMed ID: 14697336
DOI: 10.1016/j.yexcr.2003.09.018
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1126
Chen L, Maruyama D, Sugiyama M, Sakai T, Mogi C, Kato M, Kurotani R, Shirasawa N, Takaki A, Renner U, Kato Y, Inoue K.
Cytological characterization of a pituitary folliculo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neuronal nitric oxide synthase expression and nitric oxide secretion
Endocrinology
2000
141(10):3603-10
PubMed ID: 11014214
DOI: 10.1210/endo.141.10.7710
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