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Cell No. : Cell Name
RCB1691 : Tpit/F1  update : 2023/01/06
CommentPituitary folliculo-stellate-like cell line. Derived from a transgenic mouse expressing temperature sensitive SV40 large T antigen.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Inoue, Kinji
Originator Inoue, Kinji
Year of deposit 2000
Animal _mouse < Mammals
Strain name C57BL
Gender Unknown
Age at sampling 1 month
Tissue anterior pituitary gland
Classification semi-normal
Recombinant recombinant
Exogene large T
Year of origin 1995
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_S513
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 2.5% FBS + 10% HS (GIBCO)
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 1

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6664  Saishu Yoshida, Masashi Higuchi, Hiroki Ueharu, Naoto Nishimura, Mitsuyoshi Tsuda, Hideji Yako, Mo Chen, Hideo Mitsuishi, Yoshiya Sano, Takako Kato, Yukio Kato  Characterization of murine pituitary-derived cell lines Tpit/F1, Tpit/E and TtT/GF  J Reprod Dev  2014  60(4):295-303  PubMed ID: 24881870   DOI: 10.1262/jrd.2014-031
7535  Chihiro Mogi, Shunsuke Miyai, Yusuke Nishimura, Hitomi Fukuro, Kotaro Yokoyama, Atsushi Takaki, Kinji Inoue  Differentiation of skeletal muscle from pituitary folliculo-stellate cells and endocrine progenitor cells  Exp Cell Res  2004  292(2):288-94  PubMed ID: 14697336   DOI: 10.1016/j.yexcr.2003.09.018
2683  Chen L, Maruyama D, Sugiyama M, Sakai T, Mogi C, Kato M, Kurotani R, Shirasawa N, Takaki A, Renner U, Kato Y, Inoue K.  Cytological characterization of a pituitary folliculo-stellate-like cell line, Tpit/F1, with special reference to adenosine triphosphate-mediated neuronal nitric oxide synthase expression and nitric oxide secretion  Endocrinology  2000  141:3603-3610  PubMed ID: 11014214   DOI: 10.1210/endo.141.10.7710

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User's Publication
12881  Nishimura N, Ueharu H, Nishihara H, Shibuya S, Yoshida S, Higuchi M, Kanno N, Horiguchi K, Kato T, Kato Y.  Search for regulatory factors of the pituitary-specific transcription factor PROP1 gene  J Reprod Dev  2016  62(1):93-102  PubMed ID: 26640231   DOI: 10.1262/jrd.2015-092

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