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Cell No. : Cell Name
RCB1690 : CPN4B  update : 2023/01/06
CommentCell line derived from embryo of Cynops pyrrhogaster.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Hirosaki University
Faculty of Agriculture and Life Science
3 Bunkyo-Cho, Hirosaki-Shi, Aomori-Ken, 036-8561 Japan
Dr.KURO-O Masaki
Fax. +81-172-39-3750
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kuro-o, Masaki
Originator Kuro-o, Masaki
Year of deposit 2000
Animal _newt < Amphibians
Strain name Japanese
Genus Cynops
Species pyrrhogaster
Gender Unknown
Age at sampling embryo
Tissue embryo/fetus, whole
Classification other
Year of origin 1995
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_7253
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium 60% MEM + 20% D.D.W. + 20% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 2 split
SC frequency Subculture : once/10 days, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
deposit info
lot info
Reference information Reference 0
User's Publication 0

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