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Cell No. : Cell Name
RCB1681 : GS-HepG2  update : 2022/04/06
CommentA subline of Hep G2. Expressing abundant glutamine synthetase. Information
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Omasa, Takeshi
Originator Omasa, T. & Suga, K.
Year of deposit 2000
Original cell Hep G2
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue liver
Disease name hepatocyte carcinoma
Classification cancer
Recombinant recombinant
Exogene pBK-CMV-GS
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_H214
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RDF (Glutamine(-)) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP, MPI, PepB
Chromosome mode 46-52(50) : 46(2),47(2),48(6),49(10),50(16),51(13)/
STR(human) OK
Reference information Reference 7
User's Publication 2

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11987  Dolores López-Terrada, Sau Wai Cheung, Milton J Finegold, Barbara B Knowles  Hep G2 is a hepatoblastoma-derived cell line  Hum Pathol  2009  40(10):1512-5  PubMed ID: 19751877   DOI: 10.1016/j.humpath.2009.07.003
2832  Takeshi Omasa, Mitsugu Yamanaka, Naoko Tanimura, Yoshio Katakura, Michimasa Kishimoto, Ken-ichi Suga, Shin Enosawa  Expression and amplification of glutamine synthetase gene endows HepG2 cells with ammonia-metabolizing activity for bioartificial liver support system  Enzyme and Microbial Technology  2004  35:519-524    DOI: 10.1016/j.enzmictec.2004.08.027
2831  Enosawa S, Suzuki S, Fujino M, Amemiya H, Omasa T, Urayama S, Tanimura N, Suga K.  An attempt to add biological functions by genetic engineering in order to produce high-performance bioreactor cells for hybrid artificial liver: transfection of glutamine synthetase into Chinese hamster ovary (CHO) cell.  Cell Transplant  1997  6(5):537-40  PubMed ID: 9331509   DOI: 10.1016/s0963-6897(97)00073-0
2830  Enisawa, S. et al    Jpn J Artif  1996  25:399-402   
1155  Knowles, B B, Howe, C C, Aden, D P  Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.  Science  1980  209:497-9  PubMed ID: 6248960   DOI: 10.1126/science.6248960
4984  Diamond L1, Kruszewski F, Aden DP, Knowles BB, Baird WM.  Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.  Carcinogenesis  1980  1(10):871-5  PubMed ID: 11219860   DOI: 10.1093/carcin/1.10.871
465  Aden, D P, Fogel A, Plotkin S, Damjanov I, Knowles, B B  Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line.  Nature  1979  282:615-6  PubMed ID: 233137   DOI: 10.1038/282615a0

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User's Publication
9019  Omasa T, Kim K, Hiramatsu S, Katakura Y, Kishimoto M, Enosawa S, Ohtake H.  Construction and evaluation of drug-metabolizing cell line for bioartificial liver support system.  Biotechnol. Prog.  2005  21:161-7  PubMed ID: 15903254   DOI: 10.1021/bp049757a
2076  Enosawa S, Suzuki S, Kakefuda T, Amemiya H  Examination of 7-ethoxycoumarin deethylation and ammonia removal activities in 31 hepatocyte cell lines.  Cell Transplant  1996  5:S39-40  PubMed ID: 8889228   DOI: 10.1016/0963-6897(96)00036-x

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