Cell No. : Cell Name
RCB1681 : GS-HepG2
update : 2024/06/10
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| Comment | A subline of Hep G2. Expressing abundant glutamine synthetase. Information |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Omasa, Takeshi
|
| Originator |
Omasa, T. & Suga, K.
|
| Year of deposit |
2000
|
| Original cell |
Hep G2
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Tissue |
liver
|
| Disease name |
hepatocyte carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
pBK-CMV-GS
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_H214
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
RDF (Glutamine(-)) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HBV) |
|
(-)
|
| Virus (HCV) |
|
(-)
|
| Isozyme |
|
LD, NP, MPI, PepB
|
| Chromosome mode |
|
46-52(50) : 46(2),47(2),48(6),49(10),50(16),51(13)/
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
10
|
| User's Publication |
1
|
| Reference |
|
11987
Dolores López-Terrada, Sau Wai Cheung, Milton J Finegold, Barbara B Knowles
Hep G2 is a hepatoblastoma-derived cell line
Hum Pathol
2009
40(10):1512-5
PubMed ID: 19751877
DOI: 10.1016/j.humpath.2009.07.003
|
|
6809
Enosawa S, Miyashita T, Saito T, Omasa T, Matsumura T.
The significant improvement of survival times and pathological parameters by bioartificial liver with recombinant HepG2 in porcine liver failure model
Cell Transplant
2006
15(10):873-80
PubMed ID: 17299991
DOI: 10.3727/000000006783981350
|
|
2832
Takeshi Omasa, Mitsugu Yamanaka, Naoko Tanimura, Yoshio Katakura, Michimasa Kishimoto, Ken-ichi Suga, Shin Enosawa
Expression and amplification of glutamine synthetase gene endows HepG2 cells with ammonia-metabolizing activity for bioartificial liver support system
Enzyme and Microbial Technology
2004
35:519-524
DOI: 10.1016/j.enzmictec.2004.08.027
|
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10554
Mitsufumi Endo, Shin Enosawa, Seiichi Suzuki, Hiroshi Amemiya, Shuichi Tsuruoka, Akio Fujimura, Shinya Hiramatsu, Takeshi Omasa, Kenichi Suga, Toshiharu Matsumura, Tatsuya Aoki, Yasuhisa Koyanagi
Coculture of hepatic and renal origin cell lines provides biohemofiltration with an active transport system of xenobiotics metabolites
Journal of Artificial Organs
2001
4:336-341
DOI: 10.1007/bf02480028
|
|
5344
Enosawa S, Miyashita T, Suzuki S, Li XK, Tsunoda M, Amemiya H, Yamanaka M, Hiramatsu S, Tanimura N, Omasa T, Suga K, Matsumura T.
Long-term culture of glutamine synthetase-transfected HepG2 cells in circulatory flow bioreactor for development of a bioartificial liver
Cell Transplant
2000
9(5):711-5
PubMed ID: 11144971
DOI: 10.1177/096368970000900520
|
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2831
Enosawa S, Suzuki S, Fujino M, Amemiya H, Omasa T, Urayama S, Tanimura N, Suga K.
An attempt to add biological functions by genetic engineering in order to produce high-performance bioreactor cells for hybrid artificial liver: transfection of glutamine synthetase into Chinese hamster ovary (CHO) cell.
Cell Transplant
1997
6(5):537-40
PubMed ID: 9331509
DOI: 10.1177/096368979700600518
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2830
Enosawa S, Suzuki S, Kakefuda T, Amemiya H.
Examination of 7-ethoxycoumarin deethylation and ammonia removal activities in 31 hepatocyte cell lines
Cell Transplant
1996
5(5 Suppl 1):S39-40
PubMed ID: 8889228
DOI: 10.1016/0963-6897(96)00036-x
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1155
Knowles, B B, Howe, C C, Aden, D P
Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.
Science
1980
209:497-9
PubMed ID: 6248960
DOI: 10.1126/science.6248960
|
|
4984
Diamond L1, Kruszewski F, Aden DP, Knowles BB, Baird WM.
Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.
Carcinogenesis
1980
1(10):871-5
PubMed ID: 11219860
DOI: 10.1093/carcin/1.10.871
|
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465
Aden, D P, Fogel A, Plotkin S, Damjanov I, Knowles, B B
Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line.
Nature
1979
282:615-6
PubMed ID: 233137
DOI: 10.1038/282615a0
|
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