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Cell No. : Cell Name
RCB1675 : Cbl^(-) DT40  update : 2024/01/17
CommentCbl deficient DT40 cells
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kurosaki, Tomohiro
Originator Kurosaki, Tomohiro
Year of deposit 2000
Another name C8-12
Original cell DT40
Animal _avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene pBluescript
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T46
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 (4mM Glutamine) + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information Reference 1
User's Publication 0


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Reference
2727  Yasuda T, Maeda A, Kurosaki M, Tezuka T, Hironaka K, Yamamoto T, Kurosaki T  Cbl suppresses B cell receptor-mediated phospholipase C (PLC)-gamma2 activation by regulating B cell linker protein-PLC-gamma2 binding.  J Exp Med  2000  191:641-50  PubMed ID: 10684856   DOI: 10.1084/jem.191.4.641

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