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Cell No. : Cell Name
RCB1668 : 293/CrmA  update : 2022/04/06
CommentA subline of 293. Expressing caspase-inhibiting CrmA gene.
Comment from the depositor
Terms and conditionsCan be used only by not-for-profit entities and only for not-for-profit academic purposes.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hamada, Hirofumi
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Age at sampling embryo
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene pRxbsr, Crm A gene
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_J499
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 63-69(50) : /64(5),65(6),66(9),67(16),68(9),69(4)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
2693  Shinoura N, Ohashi M, Yoshida Y, Asai A, Kirino T, Saito I, Hamada H  Construction, propagation, and titer estimation of recombinant adenoviruses carrying proapoptotic genes.  Hum Gene Ther  1998  9:2683-9  PubMed ID: 9874266   DOI: 10.1089/hum.1998.9.18-2683
4972  Graham FL, Harrison T, Williams J.  Defective transforming capacity of adenovirus type 5 host-range mutants.  Virology  1978  86(1):10-21  PubMed ID: 664220   DOI: 10.1016/0042-6822(78)90003-x
2415  Harrison T, Graham F, Williams J  Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.  Virology  1977  77:319-29  PubMed ID: 841862  
2414  Graham, F L, Smiley J, Russell, W C, Nairn R  Characteristics of a human cell line transformed by DNA from human adenovirus type 5.  J Gen Virol  1977  36:59-74  PubMed ID: 886304  

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