Cell No. : Cell Name
RCB1668 : 293/CrmA
update : 2023/01/06
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| Comment | A subline of 293. Expressing caspase-inhibiting CrmA gene. |
| Comment from the depositor | |
| Terms and conditions | Can be used only by not-for-profit entities and only for not-for-profit academic purposes. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Hamada, Hirofumi
|
| Year of deposit |
2000
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Age at sampling |
embryo
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| Tissue |
kidney
|
| Classification |
transformed
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| Recombinant |
recombinant
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| Exogene |
pRxbsr, Crm A gene
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_J499
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (low glucose) + 10% FBS
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
|
| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
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63-69(50) : /64(5),65(6),66(9),67(16),68(9),69(4)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
0
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| Reference |
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2693
Shinoura N, Ohashi M, Yoshida Y, Asai A, Kirino T, Saito I, Hamada H
Construction, propagation, and titer estimation of recombinant adenoviruses carrying proapoptotic genes.
Hum Gene Ther
1998
9:2683-9
PubMed ID: 9874266
DOI: 10.1089/hum.1998.9.18-2683
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4972
Graham FL, Harrison T, Williams J.
Defective transforming capacity of adenovirus type 5 host-range mutants.
Virology
1978
86(1):10-21
PubMed ID: 664220
DOI: 10.1016/0042-6822(78)90003-x
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2415
Harrison T, Graham F, Williams J
Host-range mutants of adenovirus type 5 defective for growth in HeLa cells.
Virology
1977
77:319-29
PubMed ID: 841862
DOI: 10.1016/0042-6822(77)90428-7
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2414
Graham, F L, Smiley J, Russell, W C, Nairn R
Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
J Gen Virol
1977
36:59-74
PubMed ID: 886304
DOI: 10.1099/0022-1317-36-1-59
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