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Cell No. : Cell Name
RCB1652 : G1MF285D  update : 2022/04/06
CommentHybridoma that produce anti-human G1MF (allotype of IgG1) antibody.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kishida, Tetsuko
Originator Kishida, Tetsuko
Year of deposit 2000
Original cell G1MF
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_T774
hybridoma Fusion_cell_1 name lymphoma
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_1 gender Male
Fusion_cell_2 name P3U1
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG 1500, HAT selection
Antibody name anti G1MF
Subclass IgG1
Immunogen F(ab')2 fragments of IgG1-enrich IgG
Antigen_name G1M F
Antigen_m.w. 150000
Antigen_character human IgG allotype G1MF
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives GIT
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 2-4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium GIT : 2 x FM1 = 1 : 1
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG1
Reference information Reference 1
User's Publication 0


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Reference
2810  Tamaki Y, Kishida T, Fukuda M  Practical method for production of monoclonal antibody to human IgG allotype G1M F and its applications in ELISAs and dot immunobinding.  J Immunol Methods  1990  128:75-9  PubMed ID: 2324505   DOI: 10.1016/0022-1759(90)90465-8

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