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Cell No. : Cell Name
RCB1649 : ΔAtm-DT40  update : 2022/04/06
CommentATM gene deleted DT40 (chicken B cells).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takeda, Shunichi
Originator Ciaran, Morrison
Year of deposit 2000
Another name #244
Original cell DT40
Animal avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene Atm-neo, Atm-puro
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_1T23
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10^(5) cells/ml
Passage ratio 1 : 10-20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10^(7) cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information Reference 1
User's Publication 0


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Reference
2741  Morrison C, Sonoda E, Takao N, Shinohara A, Yamamoto K, Takeda S  The controlling role of ATM in homologous recombinational repair of DNA damage.  EMBO J  2000  19:463-71  PubMed ID: 10654944  

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