Back Back
Cell No. : Cell Name
RCB1648 : Hep G2  update : 2022/04/06
CommentHuman cell line derived from hepatoblastoma. Secretes several products such as albumin, alpha2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011430 prior to order. Information
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011430 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 15 years
Tissue liver
Disease name hepatocyte carcinoma
Classification cancer
History overseas, Dr.Knowles (Wistar Institute, Ohiladelphoa) -> ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0027
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4-8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
Chromosome mode 51-53(50) : 51(12).52(22),53(16)
STR(human) OK
Reference information Reference 4
User's Publication 101

To topTop
11987  Dolores López-Terrada, Sau Wai Cheung, Milton J Finegold, Barbara B Knowles  Hep G2 is a hepatoblastoma-derived cell line  Hum Pathol  2009  40(10):1512-5  PubMed ID: 19751877
1155  Knowles, B B, Howe, C C, Aden, D P  Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.  Science  1980  209:497-9  PubMed ID: 6248960
4984  Diamond L1, Kruszewski F, Aden DP, Knowles BB, Baird WM.  Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.  Carcinogenesis  1980  1(10):871-5  PubMed ID: 11219860
465  Aden, D P, Fogel A, Plotkin S, Damjanov I, Knowles, B B  Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line.  Nature  1979  282:615-6  PubMed ID: 233137

To topTop
User's Publication
20482  Bolatkan A, Asada K, Kaneko S, Suvarna K, Ikawa N, Machino H, Komatsu M, Shiina S, Hamamoto R.  Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules.  Int J Oncol  2022  60(1)  PubMed ID: 34913069
20514  Takezawa T, Uzu M.  HepG2-NIAS cells, a new subline of HepG2 cells that can enhance not only CYP3A4 activity but also expression of drug transporters and form bile canaliculus-like networks by the oxygenation culture via a collagen vitrigel membrane.  J Toxicol Sci  2022  47(1):39-50  PubMed ID: 34987140
20608  Nagatomo A, Inoue N, Konno T, Xu Y, Sakamoto C, Sone M, Shibasaka A, Muraoka O, Ninomiya K, Yoshikawa M, Manse Y, Morikawa T.  Ursane-type triterpene oligoglycosides with anti-hepatosteatosis and anti-hyperlipidemic activity from the leaves of Ilex paraguariensis A. St.-Hil.  J Nat Med  2022    PubMed ID: 35292883
20639  Okikawa S, Morine Y, Saito Y, Yamada S, Tokuda K, Teraoku H, Miyazaki K, Yamashita S, Ikemoto T, Imura S, Shimada M.  Inhibition of the VEGF signaling pathway attenuates tumor‑associated macrophage activity in liver cancer.  Oncol Rep  2022  47(4)  PubMed ID: 35169858
20691  Kajiwara K, Osaki H, Greßies S, Kuwata K, Kim JH, Gensch T, Sato Y, Glorius F, Yamaguchi S, Taki M.  A negative-solvatochromic fluorescent probe for visualizing intracellular distributions of fatty acid metabolites.  Nat Commun  2022  13(1):2533  PubMed ID: 35534485
16451  Kitano H, Kawabe Y, Kamihira M.  HepG2-Based Designer Cells with Heat-Inducible Enhanced Liver Functions  Cells  2022  11(7):1194  PubMed ID: 35406758
13301  Kobayashi Y, Nirasawa K, Negishi Y, Asayama S.  Noncorrelative relation between in vitro and in vivo for plasmid DNA transfection by succinylated polyethylenimine muscular injection.  J Biomater Sci Polym Ed  2021  32(3):405-416  PubMed ID: 33074050
13453  SeiyaTakemoto, Masataka Nakano, Kaori Nozaki, Tatsuki Fukami, Miki Nakajima  Adenosine deaminases acting on RNA modulate the expression of the human pregnane X receptor  Drug Metabolism and Pharmacokinetics  2021    PubMed ID: 33515843
20006  Mori A, Kobayashi Y, Nirasawa K, Negishi Y, Asayama S.  Structure-Activity Relationship of Mono-Ion Complexes for Plasmid DNA Delivery by Muscular Injection.  Pharmaceutics  2021  13(1)  PubMed ID: 33430003
20035  Ito N, Yamada M, Morishita K, Nojima S, Motooka K, Sakata N, Asuka T, Otsu R, Takamatsu S, Kamada Y, Mori S, Akita H, Eguchi H, Morii E, Miyoshi E.  Identification of fucosylated haptoglobin-producing cells in pancreatic cancer tissue and its molecular mechanism.  Glycoconj J  2021  38(1):45-54  PubMed ID: 33523362
20110  Watanabe Y, Tanabe A, Hamakubo T, Nagatoishi S, Tsumoto K.  Development of biparatopic bispecific antibody possessing tetravalent scFv-Fc capable of binding to ROBO1 expressed in hepatocellular carcinoma cells.  J Biochem  2021    PubMed ID: 33844018
20136  El-Mezayen H, Yamamura K, Yusa T, Nakao Y, Uemura N, Kitamura F, Itoyama R, Yamao T, Higashi T, Hayashi H, Imai K, Chikamoto A, Yamashita YI, Baba H.  MicroRNA-25 Exerts an Oncogenic Function by Regulating the Ubiquitin Ligase Fbxw7 in Hepatocellular Carcinoma.  Ann Surg Oncol  2021    PubMed ID: 33886022
15116  Toshiaki TAKEZAWA  Revolution in Cell Culture Technology Based on a Novel Concept for Enclosing Cells using Collagen Vitrigel Membranes  Nano Biomedicine  2021  13:1-7 
15515  Qiu R, Murata S, Cheng C, Mori A, Nie Y, Mikami S, Hasegawa S, Tadokoro T, Okamoto S, Taniguchi H.  A Novel Orthotopic Liver Cancer Model for Creating a Human-like Tumor Microenvironment  Cancers (Basel)  2021  13(16):3997  PubMed ID: 34439154
16135  Azuma R, Yamasaki T, Sano K, Munekane M, Matsuoka Y, Yamada KI, Mukai T.  A radioiodinated nitroxide probe with improved stability against bioreduction for in vivo detection of lipid radicals  Free Radic Biol Med  2021  163:297-305  PubMed ID: 33359688
20621  Kodama S, Yoshii N, Ota A, Takeshita JI, Yoshinari K, Ono A.  Association between in vitro nuclear receptor-activating profiles of chemical compounds and their in vivo hepatotoxicity in rats.  J Toxicol Sci  2021  46(12):569-587  PubMed ID: 34853243
16527  Takeba Y, Ohta Y, Ootaki M, Kobayashi T, Kida K, Watanabe M, Koizumi S, Otsubo T, Iiri T, Matsumoto N.  Identification of interleukin-16 production on tumor aggravation in hepatocellular carcinoma by a proteomics approach  Tumour Biol  2021  43(1):309-325  PubMed ID: 34897107
11062  Rahman Md Moshikur, Md Raihan Chowdhury, Rie Wakabayashi, Yoshiro Tahara, Noriho Kamiya, Muhamma Moniruzzaman, Masahiro Goto  Ionic liquids with N-methyl-2-pyrrolidonium cation as an enhancer for topical drug delivery: Synthesis, characterization, and skin-penetration evaluation  Journal of Molecular Liquids  2020  299:112166 
12988  Ramadhan W, Kagawa G, Moriyama K, Wakabayashi R, Minamihata K, Goto M, Kamiya N.  Construction of higher-order cellular microstructures by a self-wrapping co-culture strategy using a redox-responsive hydrogel.  Sci Rep  2020    PubMed ID: 32317652
13110  Ramadhan W, Ohama Y, Minamihata K, Moriyama K, Wakabayashi R, Goto M, Kamiya N.  Redox-responsive functionalized hydrogel marble for the generation of cellular spheroids.  J Biosci Bioeng  2020    PubMed ID: 32636145
13491  Masahiro Akiyama, Takamitsu Unoki, Yoshito Kumagai  Combined exposure to environmental electrophiles enhances cytotoxicity and consumption of persulfide  Fundamental Toxicological Sciences  2020  7 :161-166 
14497  Yamahira S, Satoh T, Yanagawa F, Tamura M, Takagi T, Nakatani E, Kusama Y, Sumaru K, Sugiura S, Kanamori T.  Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device  R Soc Open Sci  2020  7(7):200027  PubMed ID: 32874617
14851  Moritani C, Kawakami K, Shimoda H, Hatanaka T, Suzaki E, Tsuboi S.  Protective Effects of Rice Peptide Oryza Peptide-P60 against Oxidative Injury through Activation of Nrf2 Signaling Pathway In Vitro and In Vivo  ACS Omega  2020  5(22):13096-13107  PubMed ID: 32548495
15192  Takagi K, Midorikawa Y, Takayama T, Abe H, Fujiwara K, Soma M, Nagase H, Miki T, Fukuda N.  Effects of Pyrrole-Imidazole Polyamides Targeting Human TGF-β1 on the Malignant Phenotypes of Liver Cancer Cells  Molecules  2020  25(12):2883  PubMed ID: 32585841
16623  Wakabayashi K, Sakurai F, Ono R, Fujiwara T, Mizuguchi H.  Development of a Novel Oncolytic Adenovirus Expressing a Short-hairpin RNA Against Cullin 4A  Anticancer Res  2020  40(1):161-168  PubMed ID: 31892564
11540  Ogura S, Tameda M, Sugimoto K, Ikejiri M, Usui M, Ito M, Takei Y.  A substitution in the pre-S1 promoter region is associated with the viral regulation of hepatitis B virus.  Virol. J.  2019    PubMed ID: 31046787
11585  Okubo S, Komori H, Kuwahara A, Ohta T, Shoyama Y, Uto T.  Screening of Crude Drugs Used in Japanese Kampo Formulas for Autophagy-Mediated Cell Survival of the Human Hepatocellular Carcinoma Cell Line.  Medicines (Basel)  2019    PubMed ID: 31163644
9810  Junko Shibato, Fumiko Takenoya, Takahiro Hirabayashi, Ai Kimura, Yusuke Iwasaki, Yoko Toyoda, Motohide Hori, Shigeru Tamogami, Randeep Rakwal, Seiji Shioda  Towards Identification of Bioactive Compounds in Cold Vacuum Extracted Double Cherry Blossom (Gosen-Sakura) Leaves  Plant Signal Behav  2019  14(10):e1644594  PubMed ID: 31342859
10371  Akito Endo, Shoichiro Asayama  Preparation of Zn2+-Chelated Carboxymethyl Poly(1-Vinylimidazole) for Intracellular Zn2+ Delivery  Journal of Minerals and Materials Characterization and Engineering  2019  7:94802,8 
10854  Hideki Iwadate, Masumi Yamada, Naoki Kimura, Rina Hashimoto, Yuya Yajima, Rie Utoh, Minoru Seki  PDMS microstencil plate-supported fabrication of ultra-thin, condensed ECM membranes for separated cell coculture on both surfaces  Sensors and Actuators B: Chemical  2019  287:486-495 
11906  Kawase A, Inoue Y, Hirosoko M, Sugihara Y, Shimada H, Iwaki M.  Decrease in Multidrug Resistance-associated Protein 2 Activities by Knockdown of Phosphatidylinositol 4-phosphate 5-kinase in Hepatocytes and Cancer Cells.  J Pharm Pharm Sci  2019    PubMed ID: 31804920
12437  Hara N, Osago H, Hiyoshi M, Kobayashi-Miura M, Tsuchiya M.  Quantitative analysis of the effects of nicotinamide phosphoribosyltransferase induction on the rates of NAD+ synthesis and breakdown in mammalian cells using stable isotope-labeling combined with mass spectrometry  PLoS One  2019  14(3):e0214000  PubMed ID: 30875389
14893  Morikawa T, Nagatomo A, Oka T, Miki Y, Taira N, Shibano-Kitahara M, Hori Y, Muraoka O, Ninomiya K.  Glucose Tolerance-Improving Activity of Helichrysoside in Mice and Its Structural Requirements for Promoting Glucose and Lipid Metabolism  Int J Mol Sci  2019  ;20(24):6322  PubMed ID: 31847420
4214  Matsumura K, Imai H, Go Y, Kusuhara M, Yamaguchi K, Shirai T, Ohshima K.  Transcriptional activation of a chimeric retrogene PIPSL in a hominoid ancestor.  Gene  2018    PubMed ID: 30096459
4216  Feng R, Morine Y, Ikemoto T, Imura S, Iwahashi S, Saito Y, Shimada M.  Nrf2 activation drive macrophages polarization and cancer cell epithelial-mesenchymal transition during interaction.  Cell Commun. Signal  2018    PubMed ID: 30180849
4222  Miyamoto D, Hara T, Hyakutake A, Nakazawa K.  Changes in HepG2 spheroid behavior induced by differences in the gap distance between spheroids in a micropatterned culture system.  J. Biosci. Bioeng.  2018    PubMed ID: 29352710
9973  Kawakami K, Moritani C, Uraji M, Fujita A, Kawakami K, Hatanaka T, Suzaki E, Tsuboi S.  Hepatoprotective effects of rice-derived peptides against acetaminophen-induced damage in mice.  J Clin Biochem Nutr  2017  60:115-120  PubMed ID: 28366990
10185  Yokoi K, Yamashita K, Ishii S, Tanaka T, Nishizawa N, Tsutsui A, Miura H, Katoh H, Yamanashi T, Naito M, Sato T, Nakamura T, Watanabe M.  Comprehensive molecular exploration identified promoter DNA methylation of the CRBP1 gene as a determinant of radiation sensitivity in rectal cancer.  Br. J. Cancer  2017  116:1046-1056  PubMed ID: 28291773
10595  Uemura T, Takasaka T, Igarashi K, Ikegaya H.  Spermine oxidase promotes bile canalicular lumen formation through acrolein production.  Sci Rep  2017  7:14841  PubMed ID: 29093526
12220  Shoichiro Asayama, Mizuki Sakata, Hiroyoshi Kawakami  Structure-activity relationship between Zn 2+-chelated alkylated poly(1-vinylimidazole) and gene transfection  J Inorg Biochem  2017  173:120-125  PubMed ID: 28511062
14592  Kusama K, Okamoto Y, Saito K, Kasahara T, Murata T, Ueno Y, Kobayashi Y, Kamada Y, Miyoshi E.  Reevaluation of Pholiota squarrosa lectin-reactive haptoglobin as a pancreatic cancer biomarker using an improved ELISA system  Glycoconj J  2017  34(4):537-544  PubMed ID: 28455724
14609  Enomoto J, Kageyama T, Osaki T, Bonalumi F, Marchese F, Gautieri A, Bianchi E, Dubini G, Arrigoni C, Moretti M, Fukuda J.  Catch-and-Release of Target Cells Using Aptamer-Conjug  Sci Rep  2017  7:43375  PubMed ID: 28266533
9576  Sugai S, Yoshikawa T, Iwama T, Tsuchiya N, Ueda N, Fujinami N, Shimomura M, Zhang R, Kaneko S, Uemura Y, Nakatsura T.  Hepatocellular carcinoma cell sensitivity to Vγ9Vδ2 T lymphocyte-mediated killing is increased by zoledronate.  Int. J. Oncol.  2016  48:1794-804  PubMed ID: 26936487
9946  Abiko Y, Lin FY, Lee H, Puga A, Kumagai Y.  Quinone-mediated induction of cytochrome P450 1A1 in HepG2 cells through increased interaction of aryl hydrocarbon receptor with aryl hydrocarbon receptor nuclear translocator.  J Toxicol Sci  2016  41:775-781  PubMed ID: 27853106
10020  Moriyama K, Naito S, Wakabayashi R, Goto M, Kamiya N.  Enzymatically prepared redox-responsive hydrogels as potent matrices for hepatocellular carcinoma cell spheroid formation.  Biotechnol J  2016  11:1452-1460  PubMed ID: 27617786
4039  Oshikata-Miyazaki A, Takezawa T.  Development of an oxygenation culture method for activating the liver-specific functions of HepG2 cells utilizing a collagen vitrigel membrane chamber.  Cytotechnology  2016  68(5):1801-11  PubMed ID: 26660096
12685  Takei T, Kitazono J, Tanaka S, Nishimata H, Yoshida M.  Necrotic regions are absent in fiber-shaped cell aggregates, approximately 100 μm in diameter  Artif Cells Nanomed Biotechnol  2016  44(1):62-5  PubMed ID: 24813225
12946  Yun YS, Tajima M, Takahashi S, Takahashi Y, Umemura M, Nakano H, Park HS, Inoue H.  Two Alkaloids from Bulbs of Lycoris sanguinea M AXIM. Suppress PEPCK Expression by Inhibiting the Phosphorylation of CREB  Phytother Res  2016  30(10):1689-1695  PubMed ID: 27388056
7373  Takei T, Aokawa R, Shigemitsu T, Kawakami K, Yoshida M.  Fabrication of uniformly cell-laden porous scaffolds using a gas-in-liquid templating technique.  J. Biosci. Bioeng.  2015  120:577-81  PubMed ID: 25912452
7456  Okina Y, Takeuchi F, Yokomichi T, Takada Y, Kataoka T.  Cardenolide aglycones inhibit tumor necrosis factor α-induced expression of intercellular adhesion molecule-1 at the translation step by blocking Na⁺/K⁺-ATPase  Biol. Pharm. Bull.  2015  38:39-47  PubMed ID: 25744456
7501  Lai YT, Sato M, Ohta S, Akamatsu K, Nakao S, Sakai Y, Ito T.  Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique.  Colloids Surf B Biointerfaces  2015  127:1-7  PubMed ID: 25635614
5372  Nakajima Y, Kanno T, Nagaya T, Kuribayashi K, Nakano T, Gotoh A, Nishizaki T.  Adenosine deaminase inhibitor EHNA exhibits a potent anticancer effect against malignant pleural mesothelioma.  Cell Physiol Biochem  2015  35(1):51-60  PubMed ID: 25547995
14967  Higashi T, Hayashi H, Ishimoto T, Takeyama H, Kaida T, Arima K, Taki K, Sakamoto K, Kuroki H, Okabe H, Nitta H, Hashimoto D, Chikamoto A, Beppu T, Baba H.  miR-9-3p plays a tumour-suppressor role by targeting TAZ (WWTR1) in hepatocellular carcinoma cells  Br J Cancer  2015  113(2):252-8  PubMed ID: 26125451
15231  Motoyama K, Hirai Y, Nishiyama R, Maeda Y, Higashi T, Ishitsuka Y, Kondo Y, Irie T, Era T, Arima H.  Cholesterol lowering effects of mono-lactose-appended β-cyclodextrin in Niemann-Pick type C disease-like HepG2 cells  Beilstein J Org Chem  2015  11:2079-86  PubMed ID: 26664628
10275  Nobumasa Hara, Tomoko Shibata, Harumi Osago, Kazuo Yamada, Mikako Tsuchiya  Formation of [nicotinamide-²H₃]NAD⁺ From [²H₄]nicotinamide and [²H₄]nicotinic Acid in Human HepG2N Cells and Involvement of ²H/¹H Exchange at the Redox Site of NAD⁺/NADH  J Nutr Sci Vitaminol (Tokyo)  2014  60(1):17-21  PubMed ID: 24759255
10281  Motomu Shoji, Kiyotaka Nakagawa, Akio Watanabe, Tsuyoshi Tsuduki, Teiko Yamada, Shigefumi Kuwahara, Fumiko Kimura, Teruo Miyazawa  Comparison of the Effects of Curcumin and Curcumin Glucuronide in Human Hepatocellular Carcinoma HepG2 Cells  Food Chem  2014  151:126-32  PubMed ID: 24423511
14194  Naoki Tanigawa, Sachiko Yano, Akira Higashibata, Yoshikazu Tsuchiya, Haruna Tanii, Noboru Ando, Koji Kubota, Mutsumu Nagase  Development and Validation of a Closed Chamber for Cell Culture Experiments in Space  Biological Sciences in Space  2014  28:1-5 
5844  Kanno T, Tanaka A, Shimizu T, Nakano T, Nishizaki T.  1-[2-(2-Methoxyphenylamino)ethylamino]-3-(naphthalene-1-yloxy)propan-2-ol as a potential anticancer drug.  Pharmacology  2013  91(5-6):339-45  PubMed ID: 23817168
12867  Hanada S, Fujioka K, Futamura Y, Manabe N, Hoshino A, Yamamoto K.  Evaluation of anti-inflammatory drug-conjugated silicon quantum dots: their cytotoxicity and biological effect  Int J Mol Sci  2013  14(1):1323-34  PubMed ID: 23306154
15922  Fujitani N, Furukawa J, Araki K, Fujioka T, Takegawa Y, Piao J, Nishioka T, Tamura T, Nikaido T, Ito M, Nakamura Y, Shinohara Y.  Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers  Proc Natl Acad Sci U S A   2013  110(6):2105-10  PubMed ID: 23345451
15936  Kondo Y, Ishitsuka Y, Kadowaki D, Fukumoto Y, Miyamoto Y, Irikura M, Hirata S, Sato K, Maruyama T, Hamasaki N, Irie T.  Phosphoenolpyruvate, a glycolytic intermediate, as a cytoprotectant and antioxidant in ex-vivo cold-preserved mouse liver: a potential application for organ preservation  J Pharm Pharmacol  2013  65(3):390-401  PubMed ID: 23356848
16610  Abiko Y, Kumagai Y.  Interaction of Keap1 modified by 2-tert-butyl-1,4-benzoquinone with GSH: evidence for S-transarylatio  Chem Res Toxicol  2013  26(7):1080-7  PubMed ID: 23718696
7683  Fujii T, Minagawa T, Shimizu T, Takeguchi N, Sakai H.  Inhibition of ecto-ATPase activity by curcumin in hepatocellular carcinoma HepG2 cells.  J Physiol Sci  2012  62:53-8  PubMed ID: 21932081
12539  Kawano Y, Nagata M, Kohno T, Ichimiya A, Iwakiri T, Okumura M, Arimori K.  Caffeine increases the antitumor effect of Cisplatin in human hepatocellular carcinoma cells  Biol Pharm Bull  2012  35(3):400-7  PubMed ID: 22382328
12727  Tamura K, Kanno T, Fujita Y, Gotoh A, Nakano T, Nishizaki T.  A(2a) adenosine receptor mediates HepG2 cell apoptosis by downregulating Bcl-X(L) expression and upregulating Bid expression  J Cell Biochem  2012  113(5):1766-75.  PubMed ID: 22213163
7642  Tagaya M, Yamazaki T, Tsuya D, Sugimoto Y, Hanagata N, Ikoma T.  Nano/microstructural effect of hydroxyapatite nanocrystals on hepatocyte cell aggregation and adhesion.  Macromol Biosci  2011  11:1586-93  PubMed ID: 22052565
7988  Yasuda A, Natsume M, Osakabe N, Kawahata K, Koga J.  Cacao polyphenols influence the regulation of apolipoprotein in HepG2 and Caco2 cells.  J. Agric. Food Chem.  2011  59:1470-6  PubMed ID: 21226458
12747  Yaguchi T, Nakano T, Gotoh A, Nishizaki T.  Adenosine promotes GATA-2-regulated p53 gene transcription to induce HepG2 cell apoptosis  Cell Physiol Biochem  2011  28(4):761-70  PubMed ID: 22178888
13678  Masuda M, Shimomura M, Kobayashi K, Kojima S, Nakatsura T.  Growth inhibition by NVP-BEZ235, a dual PI3K/mTOR inhibitor, in hepatocellular carcinoma cell lines  Oncol Rep  2011  26(5):1273-9  PubMed ID: 21725613
8211  Okuyama T, Yamazoe H, Mochizuki N, Khademhosseini A, Suzuki H, Fukuda J.  Preparation of arrays of cell spheroids and spheroid-monolayer cocultures within a microfluidic device.  J. Biosci. Bioeng.  2010  110:572-6  PubMed ID: 20591731
8234  Okuyama T, Yamazoe H, Seto Y, Suzuki H, Fukuda J.  Cell micropatterning inside a microchannel and assays under a stable concentration gradient.  J. Biosci. Bioeng.  2010  110:230-7  PubMed ID: 20547384
8237  Sakai Y, Yoshida S, Yoshiura Y, Mori R, Tamura T, Yahiro K, Mori H, Kanemura Y, Yamasaki M, Nakazawa K.  Effect of microwell chip structure on cell microsphere production of various animal cells.  J. Biosci. Bioeng.  2010  110:223-9  PubMed ID: 20547385
8454  Yang D, Yaguchi T, Lim CR, Ishizawa Y, Nakano T, Nishizaki T.  Tuning of apoptosis-mediator gene transcription in HepG2 human hepatoma cells through an adenosine signal.  Cancer Lett.  2010  291:225-9  PubMed ID: 19900759
12754  Yang D, Yaguchi T, Nakano T, Nishizaki T.  Adenosine-induced caspase-3 activation by tuning Bcl-XL/DIABLO/IAP expression in HuH-7 human hepatoma cells  Cell Biol Toxicol  2010  26(4):319-30  PubMed ID: 20063052
12757  Sato W, Suzuki H, Sasaki T, Kumagai T, Sakaguchi S, Mizugaki M, Miyairi S, Yamazoe Y, Nagata K.  Construction of a system that simultaneously evaluates CYP1A1 and CYP1A2 induction in a stable human-derived cell line using a dual reporter plasmid  Drug Metab Pharmacokinet  2010  25(2):180-9  PubMed ID: 20460824
15367  Miki T, Iguchi M, Akiba K, Hosono M, Sobue T, Danbara H, Okada N.  Chromobacterium pathogenicity island 1 type III secretion system is a major virulence determinant for Chromobacterium violaceum-induced cell death in hepatocytes  Mol Microbiol  2010  77(4):855-72  PubMed ID: 20545857
16392  Plessy C, Bertin N, Takahashi H, Simone R, Salimullah M, Lassmann T, Vitezic M, Severin J, Olivarius S, Lazarevic D, Hornig N, Orlando V, Bell I, Gao H, Dumais J, Kapranov P, Wang H, Davis CA, Gingeras TR, Kawai J, Daub CO, Hayashizaki Y, Gustincich S, Carninci P.  Linking promoters to functional transcripts in small samples with nanoCAGE and CAGEscan  Nat Methods  2010  7(7):528-34  PubMed ID: 20543846
8705  Hattori N, Suzuki T, Jinno S, Okeya H, Ishikawa A, Kondo C, Hayashi T, Ito M, Kanamori T, Kawai T, Noguchi T.  Methyl methacrylate activates the Gsta1 promoter.  J. Dent. Res.  2008  87:1117-21  PubMed ID: 19029078
8720  Mori R, Sakai Y, Nakazawa K.  Micropatterned organoid culture of rat hepatocytes and HepG2 cells.  J. Biosci. Bioeng.  2008  106:237-42  PubMed ID: 18929998
8765  Saigo K, Yoshida K, Ikeda R, Sakamoto Y, Murakami Y, Urashima T, Asano T, Kenmochi T, Inoue I.  Integration of hepatitis B virus DNA into the myeloid/lymphoid or mixed-lineage leukemia (MLL4) gene and rearrangements of MLL4 in human hepatocellular carcinoma.  Hum. Mutat.  2008  29:703-8  PubMed ID: 18320596
8786  Tamura T, Sakai Y, Nakazawa K.  Two-dimensional microarray of HepG2 spheroids using collagen/polyethylene glycol micropatterned chip.  J Mater Sci Mater Med  2008  19:2071-7  PubMed ID: 17968500
12797  Yoshinari K, Ueda R, Kusano K, Yoshimura T, Nagata K, Yamazoe Y.  Omeprazole transactivates human CYP1A1 and CYP1A2 expression through the common regulatory region containing multiple xenobiotic-responsive elements  Biochem Pharmacol  2008  76(1):139-45  PubMed ID: 18502397
13723    Nrf2-dependent induction of human ABC transporter ABCG2 and heme oxygenase-1 in HepG2 cells by photoactivation of porphyrins: biochemical implications for cancer cell response to photodynamic therapy  J Exp Ther Oncol  2008  7(2):153-67  PubMed ID: 18771089
14931  Sasaki M, Ikeda H, Itatsu K, Yamaguchi J, Sawada S, Minato H, Ohta T, Nakanuma Y.  The overexpression of polycomb group proteins Bmi1 and EZH2 is associated with the progression and aggressive biological behavior of hepatocellular carcinoma  Lab Invest  2008  88(8):873-82  PubMed ID: 18591938
8860  Horiuchi S, Aoyama Y.  Systematic lactose-functionalization of amphiphilic octaamine macrocycle as a gene carrier. Optimization of the charge, size, toxicity, and receptor factors for hepatocyte targeting.  J Control Release  2006  116:107-14  PubMed ID: 16905218
3413  Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72  PubMed ID: 16428294
2892  Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E  Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis  J Biochem (Tokyo)  2005  137:17-25  PubMed ID: 15713879
3407  Ito, Mikiko, Sakai, Yuko, Furumoto, Mari, Segawa, Hiroko, Haito, Sakiko, Yamanaka, Setsuko, Nakamura, Rie, Kuwahata, Masashi, Miyamoto, Ken-ichi  Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells.  Am J Physiol Endocrinol Metab  2005  288:E1101-9  PubMed ID: 15671080
14325  Sugatani J, Sueyoshi T, Negishi M, Miwa M.  Regulation of the human UGT1A1 gene by nuclear receptors constitutive active/androstane receptor, pregnane X receptor, and glucocorticoid receptor  Methods Enzymol  2005  400:92-104  PubMed ID: 16399345
2894  Ito, Akio, Sasaguri, Yasuyuki, Kitada, Sakae, Kusaka, Yoshitomo, Kuwano, Kyoko, Masutomi, Kenjiro, Mizuki, Eiichi, Akao, Tetsuyuki, Ohba, Michio  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J Biol Chem  2004  279:21282-6  PubMed ID: 15026424
9172  Harada K, Ohba K, Ozaki S, Isse K, Hirayama T, Wada A, Nakanuma Y.  Peptide antibiotic human beta-defensin-1 and -2 contribute to antimicrobial defense of the intrahepatic biliary tree.  Hepatology  2004  40:925-32  PubMed ID: 15382127
12842  Sakai Y, Fukuda O, Choi SH, Sakoda A.  Use of a perfusion co-culture system consisting of Caco-2 and Hep G2 cell compartments for the kinetic analysis of benzo[a]pyrene toxicity  Altern Lab Anim  2004  32 Suppl 1A:99-103  PubMed ID: 23577440
12844  Sugatani J, Yamakawa K, Tonda E, Nishitani S, Yoshinari K, Degawa M, Abe I, Noguchi H, Miwa M.  The induction of human UDP-glucuronosyltransferase 1A1 mediated through a distal enhancer module by flavonoids and xenobiotics  Biochem Pharmacol  2004  67(5):989-1000  PubMed ID: 15104253
12845  Choi S, Nishikawa M, Sakoda A, Sakai Y.  Feasibility of a simple double-layered coculture system incorporating metabolic processes of the intestine and liver tissue: application to the analysis of benzo[a]pyrene toxicity  Toxicol In Vitro  2004  18(3):393-402  PubMed ID: 15046787
3793  Yoshida S, Honda A, Matsuzaki Y, Fukushima S, Tanaka N, Takagiwa A, Fujimoto Y, Miyazaki H, Salen G.  Anti-proliferative action of endogenous dehydroepiandrosterone metabolites on human cancer cell lines.  Steroids  2003  68(1):73-83  PubMed ID: 12475725
3794  Sakai Y, Fukuda O, Choi SH, Sakoda A.  Development of a biohybrid simulator for absorption and biotransformation processes in humans based on in vitro models of small intestine and liver tissues.  J Artif Organs  2003  6(4):273-81  PubMed ID: 14691670
14329  Hata K, Hori K, Ogasawara H, Takahashi S.  Anti-leukemia activities of Lup-28-al-20(29)-en-3-one, a lupane triterpene  Toxicol Lett  2003  143(1):1-7  PubMed ID: 12697374
9400  Ikeda T, Zhang J, Chano T, Mabuchi A, Fukuda A, Kawaguchi H, Nakamura K, Ikegawa S.  Identification and characterization of the human long form of Sox5 (L-SOX5) gene.  Gene  2002  298:59-68  PubMed ID: 12406576
3787  Mikiko Shimizu, Hiro-omi Tamura  Identification and Localization of Two Hydroxysteroid Sulfotransferases in the Human Brain  Journal of Health Science  2002  48:467-472 
3773  Hirano R, Igarashi O, Kondo K, Itakura H, Matsumoto A.  Regulation by long-chain fatty acids of the expression of cholesteryl ester transfer protein in HepG2 cells.  Lipids  2001  36(4):401-6  PubMed ID: 11383693
6400  Hatano H, Kawashima H, Ogose A, Hotta T, Endo N.  A PCR-ELISA assay for the detection of disseminated osteosarcoma cells in a mouse metastatic model.  J Orthop Sci  2001  6(3):269-75  PubMed ID: 11484122

Back Back Return Top Page