Cell No. : Cell Name RCB1647 : RAJI  update : 2025/01/16
Comment			Burkitt's lymphoma. Sensitive to LAK cells but resistant to NK cells. Back up of culture ECA2673. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a 'non-producer'; the EBV genome carries deletions attributed to preventing the formation of virus particles. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85011429 prior to order.
Comment from the depositor
Terms and conditions			1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011429 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	ECACC
		Year of deposit	2000
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	11 years
		Tissue	maxilla
		Disease name	Burkitt's lymphoma
		Classification	cancer
		History	overseas, ECACC -> RIKEN Cell bank
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_0511
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		LT 400
		Virus (HTLV-1)		(-)
		Virus (EBV)		(+)
		Isozyme		LD, NP, AST
		Chromosome mode		46-50(50) : 46(4),47(13),48(16),49(15),50(2)
		STR(human)		OK
Reference information		Reference	5
		User's Publication	36

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Reference

1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890   DOI: 10.1084/jem.143.6.1528

4739  Huang CC, Minowada J.  Differential effects of infection with herpes simplex virus on the chromosomes of human hematopoietic cell lines.  Cancer Res  1972  32(6):1218-25  PubMed ID: 4337885

2472      Trans N Y Acad Sci  1966  29:61

479  Epstein, M A, Achong, B G, Barr, Y M, Zajac B, Henle G, Henle W  Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji).  J Natl Cancer Inst  1966  37:547-59  PubMed ID: 4288580

972  Pulvertaft,R.J.V.  Cytology of Burkitt's tumor (African lymphoma).  Lancet  1964  1:238-240  PubMed ID: 14086209   DOI: 10.1016/s0140-6736(64)92345-1

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User's Publication

20569  Sugiura D, Okazaki IM, Maeda TK, Maruhashi T, Shimizu K, Arakaki R, Takemoto T, Ishimaru N, Okazaki T.  PD-1 agonism by anti-CD80 inhibits T cell activation and alleviates autoimmunity.  Nat Immunol  2022    PubMed ID: 35145298   DOI: 10.1038/s41590-021-01125-7

16783  Wong TY, Menaga S, Huang CF, Ho SHA, Gan SC, Lim YM.  2-Methoxy-1,4-naphthoquinone (MNQ) regulates cancer key genes of MAPK, PI3K, and NF-κB pathways in Raji cells  Genomics Inform  2022  20(1):e7  PubMed ID: 35399006   DOI: 10.5808/gi.21041

16945  Yano H, Fujiwara Y, Horlad H, Pan C, Kai K, Niino D, Ohsawa K, Higashi M, Nosaka K, Okuno Y, Tamaru JI, Mukasa A, Matsuoka M, Komohara Y.  Blocking cholesterol efflux mechanism is a potential target for antilymphoma therapy  Cancer Sci  2022  113(6):2129-2143  PubMed ID: 35343027   DOI: 10.1111/cas.15349

14298  Lau WM, Subramaniam M, Goh HH, Lim YM.  Temporal gene expression profiling of maslinic acid-treated Raji cells  Mol Omics  2021  17(2):252-259  PubMed ID: 33346776   DOI: 10.1039/d0mo00168f

13136  Isayama T, Etoh H, Kishimoto N, Takasaki T, Kuratani A, Ikuta T, Tatefuji T, Takamune N, Muneoka A, Takahashi Y, Misumi S.  10-Hydroxydecanoic Acid Potentially Elicits Antigen-Specific IgA Responses.  Biol Pharm Bull  2020    PubMed ID: 32741940   DOI: 10.1248/bpb.b20-00101

11518  Hayano A, Takashima Y, Yamanaka R.  Cell-type-specific sensitivity of bortezomib in the methotrexate-resistant primary central nervous system lymphoma cells.  Int. J. Clin. Oncol.  2019    PubMed ID: 30993483   DOI: 10.1007/s10147-019-01451-9

6897  Takashima Y, Yoshimura T, Kano Y, Hayano A, Hondoh H, Ikenaka K, Yamanaka R.  Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.  BMC Cancer  2019  19(1):910  PubMed ID: 31510952   DOI: 10.1186/s12885-019-6129-8

11256  Maruhashi T, Okazaki IM, Sugiura D, Takahashi S, Maeda TK, Shimizu K, Okazaki T.  LAG-3 inhibits the activation of CD4+ T cells that recognize stable pMHCII through its conformation-dependent recognition of pMHCII.  Nat. Immunol.  2018    PubMed ID: 30349037   DOI: 10.1038/s41590-018-0217-9

11858  Shinojima N, Fujimoto K, Makino K, Todaka K, Yamada K, Mikami Y, Oda K, Nakamura K, Jono H, Kuratsu JI, Nakamura H, Yano S, Mukasa A.  Clinical significance of polyglutamylation in primary central nervous system lymphoma.  Acta Neuropathol Commun  2018    PubMed ID: 29475458   DOI: 10.1186/s40478-018-0522-4

10269  Komohara Y, Ma C, Yano H, Pan C, Horlad H, Saito Y, Ohnishi K, Fujiwara Y, Okuno Y, Nosaka K, Shimosaki S, Morishita K, Matsuoka M, Wakayama T, Takeya M.  Cell adhesion molecule-1 (CADM1) expressed on adult T-cell leukemia/lymphoma cells is not involved in the interaction with macrophages  J Clin Exp Hematop  2017  57:15-20  PubMed ID: 28420814   DOI: 10.3960/jslrt.17003

12230  Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Masataka Oda, Hisanori Domon, Yoko Iwase, Tadashi Sawami, Kazunari Matsueda, Yutaka Terao, Hayato Ohshima, Hirofumi Noguchi, Haruaki Hayasaki  Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers  Arch Oral Biol  2017  81:110-120  PubMed ID: 28500952   DOI: 10.1016/j.archoralbio.2017.04.033

13994  Horlad H, Ma C, Yano H, Pan C, Ohnishi K, Fujiwara Y, Endo S, Kikukawa Y, Okuno Y, Matsuoka M, Takeya M, Komohara Y.  An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma  Cancer Sci  2016  107(11):1696-1704  PubMed ID: 27564404   DOI: 10.1111/cas.13065

7572  Yew WT, Kitson L, Hock AHS, Chiew GS, Mooi LY  2-Methoxy-1, 4-Naphthoquinone (MNQ) Suppresses Protein Kinase C βI, δ, and ζ Expression in Raji Cells.  J App Pharm Sci.  2015  5:001-005    DOI: 10.7324/JAPS.2015.50801

19086  Nakayama F, Umeda S, Ichimiya T, Kamiyama S, Hazawa M, Yasuda T, Nishihara S, Imai T.  Sulfation of keratan sulfate proteoglycan reduces radiation-induced apoptosis in human Burkitt's lymphoma cell lines  FEBS Lett  2013  587(2):231-7  PubMed ID: 23238079   DOI: 10.1016/j.febslet.2012.12.002

15339  Bai B, Horlad H, Saito Y, Ohnishi K, Fujiwara Y, Takeya M, Komohara Y.  Role of Stat3 activation in cell-cell interaction between B-cell lymphoma and macrophages: the in vitro study  J Clin Exp Hematop  2013  53(2):127-33  PubMed ID: 23995109   DOI: 10.3960/jslrt.53.127

19081  Machino T, Okoshi Y, Miyake Y, Akatsuka Y, Chiba S.  HLA-C matching status does not affect rituximab-mediated antibody-dependent cellular cytotoxicity by allogeneic natural killer Cells  Immunol Invest  2012  41(8):831-46  PubMed ID: 22676066   DOI: 10.3109/08820139.2012.691148

7977  Watanabe A, Tatematsu M, Saeki K, Shibata S, Shime H, Yoshimura A, Obuse C, Seya T, Matsumoto M.  Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.  J. Biol. Chem.  2011  286:10702-11  PubMed ID: 21266579   DOI: 10.1074/jbc.M110.185793

8171  Hsum YW, Yew WT, Hong PL, Soo KK, Hoon LS, Chieng YC, Mooi LY.  Cancer chemopreventive activity of maslinic acid: suppression of COX-2 expression and inhibition of NF-κB and AP-1 activation in Raji cells.  Planta Med.  2011  77:152-7  PubMed ID: 20669087   DOI: 10.1055/s-0030-1250203

8486  Hazawa M, Takahashi K, Wada K, Mori T, Kawahara N, Kashiwakura I.  Structure-activity relationships between the Aconitum C20-diterpenoid alkaloid derivatives and the growth suppressive activities of Non-Hodgkin's lymphoma Raji cells and human hematopoietic stem/progenitor cells.  Invest New Drugs  2011  29:1-8  PubMed ID: 19784550   DOI: 10.1007/s10637-009-9327-4

18937  Komohara Y, Horlad H, Ohnishi K, Ohta K, Makino K, Hondo H, Yamanaka R, Kajiwara K, Saito T, Kuratsu J, Takeya M.  M2 macrophage/microglial cells induce activation of Stat3 in primary central nervous system lymphoma  J Clin Exp Hematop  2011  51(2):93-9  PubMed ID: 22104307   DOI: 10.3960/jslrt.51.93

12743  Masuda K, Kajikawa A, Igimi S.  Establishment and Evaluation of an in vitro M Cell Model using C2BBe1 Cells and Raji Cells  Biosci Microflora  2011  30(2):37-4  PubMed ID: 25045312   DOI: 10.12938/bifidus.30.37

18891  Miyake Y, Okoshi Y, Machino T, Chiba S.  Treatment of central nervous system lymphoma in rats with intraventricular rituximab and serum  Int J Hematol  2010  92(3):474-80  PubMed ID: 20820968   DOI: 10.1007/s12185-010-0669-7

19085  Wang L, Nishihara H, Kimura T, Kato Y, Tanino M, Nishio M, Obara M, Endo T, Koike T, Tanaka S.  DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma  Biochem Biophys Res Commun   2010  395(1):111-5  PubMed ID: 20350533   DOI: 10.1016/j.bbrc.2010.03.148

19125  Shiraishi Y, Gotoh K, Towata T, Shimasaki T, Suzu S, Kojima A, Okada S.  Therapeutic effects of γ-irradiation in a primary effusion lymphoma mouse model  Exp Ther Med  2010  1(1):79-84  PubMed ID: 23136597   DOI: 10.3892/etm_00000014

14410  Uchiyama S, Suzuki Y, Otake K, Yokoyama M, Ohta M, Aikawa S, Komatsu M, Sawada T, Kagami Y, Morishima Y, Fukui K.  Development of novel humanized anti-CD20 antibodies based on affinity constant and epitope  Cancer Sci  2010  101(1):201-9  PubMed ID: 19930155   DOI: 10.1111/j.1349-7006.2009.01392.x

18297  Hazawa M, Wada K, Takahashi K, Mori T, Kawahara N, Kashiwakura I.  Suppressive effects of novel derivatives prepared from Aconitum alkaloids on tumor growth  Invest New Drugs  2009  27(2):111-9  PubMed ID: 18551247   DOI: 10.1007/s10637-008-9141-4

8561  Takahashi-Makise N, Suzu S, Hiyoshi M, Ohsugi T, Katano H, Umezawa K, Okada S.  Biscoclaurine alkaloid cepharanthine inhibits the growth of primary effusion lymphoma in vitro and in vivo and induces apoptosis via suppression of the NF-kappaB pathway.  Int. J. Cancer  2009  125:1464-72  PubMed ID: 19521981   DOI: 10.1002/ijc.24521

12791  Fukuzawa M, Yamaguchi R, Hide I, Chen Z, Hirai Y, Sugimoto A, Yasuhara T, Nakata Y.  Possible involvement of long chain fatty acids in the spores of Ganoderma lucidum (Reishi Houshi) to its anti-tumor activity  Biol Pharm Bull  2008  31(10):1933-7  PubMed ID: 18827358   DOI: 10.1248/bpb.31.1933

13126  Ichihara H, Nagami H, Kiyokawa T, Matsumoto Y, Ueoka R.  Chemotherapy using hybrid liposomes along with induction of apoptosis  Anticancer Res  2008  28(2B):1187-95  PubMed ID: 18505055

16559  Nishida M, Usuda S, Okabe M, Miyakoda H, Komatsu M, Hanaoka H, Teshigawara K, Niwa O.  Characterization of novel murine anti-CD20 monoclonal antibodies and their comparison to 2B8 and c2B8 (rituximab)  Int J Oncol  2007  31(1):29-40  PubMed ID: 17549402

19312  Yabuta N, Onda H, Watanabe M, Yoshioka N, Nagamori I, Funatsu T, Toji S, Tamai K, Nojima H.  Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest  Nucleic Acids Res  2006  34(17):4878-92.  PubMed ID: 16973895   DOI: 10.1093/nar/gkl651

16902  Fujita K, Tsujimura A, Miyagawa Y, Kiuchi H, Matsuoka Y, Takao T, Takada S, Nonomura N, Okuyama A.  Isolation of germ cells from leukemia and lymphoma cells in a human in vitro model: potential clinical application for restoring human fertility after anticancer therapy  Cancer Res  2006  66(23):11166-71  PubMed ID: 17145860   DOI: 10.1158/0008-5472.CAN-06-2326

3407  Ito, Mikiko, Sakai, Yuko, Furumoto, Mari, Segawa, Hiroko, Haito, Sakiko, Yamanaka, Setsuko, Nakamura, Rie, Kuwahata, Masashi, Miyamoto, Ken-ichi  Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells.  Am J Physiol Endocrinol Metab  2005  288:E1101-9  PubMed ID: 15671080   DOI: 10.1152/ajpendo.00502.2004

2845  Shirakawa, Jun, Shibuya, Kazuko, Shibuya, Akira  Requirement of the serine at residue 329 for lipid raft recruitment of DNAM-1 (CD226).  Int Immunol  2005  17:217-23  PubMed ID: 15684041   DOI: 10.1093/intimm/dxh199

3379  Kochi, Yuta, Yamada, Ryo, Suzuki, Akari, Harley, John B, Shirasawa, Senji, Sawada, Tetsuji, Bae, Sang-Cheol, Tokuhiro, Shinya, Chang, Xiaotian, Sekine, Akihiro, Takahashi, Atsushi, Tsunoda, Tatsuhiko, Ohnishi, Yozo, Kaufman, Kenneth M, Kang, Changsoo Paul, Kang, Changwon, Otsubo, Shigeru, Yumura, Wako, Mimori, Akio, Koike, Takao, Nakamura, Yusuke, Sasazuki, Takehiko, Yamamoto, Kazuhiko  A functional variant in FCRL3, encoding Fc receptor-like 3, is associated with rheumatoid arthritis and several autoimmunities.  Nat Genet  2005  37:478-85  PubMed ID: 15838509   DOI: 10.1038/ng1540

16563  Nakao Y, Funami K, Kikkawa S, Taniguchi M, Nishiguchi M, Fukumori Y, Seya T, Matsumoto M.  Surface-expressed TLR6 participates in the recognition of diacylated lipopeptide and peptidoglycan in human cells  J Immunol  2005  174(3):1566-73  PubMed ID: 15661917   DOI: 10.4049/jimmunol.174.3.1566