Cell No. : Cell Name RCB1647 : RAJI  update : 2019/12/16
Comment			Burkitt's lymphoma. Sensitive to LAK cells but resistant to NK cells. Back up of culture ECA2673. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a 'non-producer'; the EBV genome carries deletions attributed to preventing the formation of virus particles. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 85011429 prior to order.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011429 prior to order. In addition, regarding commercial use and use for patent filing the users of this cell line must contact RIKEN BRC prior to order.
Remarks
Order Form			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
	Basic information	Depositor	ECACC
		Year of deposit	2000
		Animal	human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	11 years
		Tissue	maxilla
		Case history	Burkitt's lymphoma
		Classification	cancer
		History	overseas, ECACC -> RIKEN Cell bank
		Lifespan	infinite
		Morphology	lymphocyte-like
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Medium and additives		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		STR(human)		OK
		Virus (HIV)		LT 400
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP, AST
		Chromosome mode		46-50(50) : 46(4),47(13),48(16),49(15),50(2)
Reference information		Reference	5
		User's Publication	12

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Reference

1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890

4739  Huang CC, Minowada J.  Differential effects of infection with herpes simplex virus on the chromosomes of human hematopoietic cell lines.  Cancer Res  1972  32(6):1218-25  PubMed ID: 4337885

2472      Trans N Y Acad Sci  1966  29:61

479  Epstein, M A, Achong, B G, Barr, Y M, Zajac B, Henle G, Henle W  Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji).  J Natl Cancer Inst  1966  37:547-59  PubMed ID: 4288580

972  Pulvertaft,R.J.V.  Cytology of Burkitt's tumor (African lymphoma).  Lancet  1964  1:238-240  PubMed ID: 14086209

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User's Publication

11518  Hayano A, Takashima Y, Yamanaka R.  Cell-type-specific sensitivity of bortezomib in the methotrexate-resistant primary central nervous system lymphoma cells.  Int. J. Clin. Oncol.  2019    PubMed ID: 30993483

6897  Takashima Y, Yoshimura T, Kano Y, Hayano A, Hondoh H, Ikenaka K, Yamanaka R.  Differential expression of N-linked oligosaccharides in methotrexate-resistant primary central nervous system lymphoma cells.  BMC Cancer  2019  19(1):910  PubMed ID: 31510952

11256  Maruhashi T, Okazaki IM, Sugiura D, Takahashi S, Maeda TK, Shimizu K, Okazaki T.  LAG-3 inhibits the activation of CD4+ T cells that recognize stable pMHCII through its conformation-dependent recognition of pMHCII.  Nat. Immunol.  2018    PubMed ID: 30349037

11858  Shinojima N, Fujimoto K, Makino K, Todaka K, Yamada K, Mikami Y, Oda K, Nakamura K, Jono H, Kuratsu JI, Nakamura H, Yano S, Mukasa A.  Clinical significance of polyglutamylation in primary central nervous system lymphoma.  Acta Neuropathol Commun  2018    PubMed ID: 29475458

10269  Komohara Y, Ma C, Yano H, Pan C, Horlad H, Saito Y, Ohnishi K, Fujiwara Y, Okuno Y, Nosaka K, Shimosaki S, Morishita K, Matsuoka M, Wakayama T, Takeya M.  Cell adhesion molecule-1 (CADM1) expressed on adult T-cell leukemia/lymphoma cells is not involved in the interaction with macrophages.  J Clin Exp Hematop  2017  57:15-20  PubMed ID: 28420814

12230  Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Masataka Oda, Hisanori Domon, Yoko Iwase, Tadashi Sawami, Kazunari Matsueda, Yutaka Terao, Hayato Ohshima, Hirofumi Noguchi, Haruaki Hayasaki  Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers  Arch Oral Biol  2017  81:110-120  PubMed ID: 28500952

8171  Hsum YW, Yew WT, Hong PL, Soo KK, Hoon LS, Chieng YC, Mooi LY.  Cancer chemopreventive activity of maslinic acid: suppression of COX-2 expression and inhibition of NF-κB and AP-1 activation in Raji cells.  Planta Med.  2011  77:152-7  PubMed ID: 20669087

8486  Hazawa M, Takahashi K, Wada K, Mori T, Kawahara N, Kashiwakura I.  Structure-activity relationships between the Aconitum C20-diterpenoid alkaloid derivatives and the growth suppressive activities of Non-Hodgkin's lymphoma Raji cells and human hematopoietic stem/progenitor cells.  Invest New Drugs  2009  29:1-8  PubMed ID: 19784550

8561  Takahashi-Makise N, Suzu S, Hiyoshi M, Ohsugi T, Katano H, Umezawa K, Okada S.  Biscoclaurine alkaloid cepharanthine inhibits the growth of primary effusion lymphoma in vitro and in vivo and induces apoptosis via suppression of the NF-kappaB pathway.  Int. J. Cancer  2009  125:1464-72  PubMed ID: 19521981

3407  Ito, Mikiko, Sakai, Yuko, Furumoto, Mari, Segawa, Hiroko, Haito, Sakiko, Yamanaka, Setsuko, Nakamura, Rie, Kuwahata, Masashi, Miyamoto, Ken-ichi  Vitamin D and phosphate regulate fibroblast growth factor-23 in K-562 cells.  Am J Physiol Endocrinol Metab  2005  288:E1101-9  PubMed ID: 15671080

2845  Shirakawa, Jun, Shibuya, Kazuko, Shibuya, Akira  Requirement of the serine at residue 329 for lipid raft recruitment of DNAM-1 (CD226).  Int Immunol  2005  17:217-23  PubMed ID: 15684041

3379  Kochi, Yuta, Yamada, Ryo, Suzuki, Akari, Harley, John B, Shirasawa, Senji, Sawada, Tetsuji, Bae, Sang-Cheol, Tokuhiro, Shinya, Chang, Xiaotian, Sekine, Akihiro, Takahashi, Atsushi, Tsunoda, Tatsuhiko, Ohnishi, Yozo, Kaufman, Kenneth M, Kang, Changsoo Paul, Kang, Changwon, Otsubo, Shigeru, Yumura, Wako, Mimori, Akio, Koike, Takao, Nakamura, Yusuke, Sasazuki, Takehiko, Yamamoto, Kazuhiko  A functional variant in FCRL3, encoding Fc receptor-like 3, is associated with rheumatoid arthritis and several autoimmunities.  Nat Genet  2005  37:478-85  PubMed ID: 15838509