Cell No. : Cell Name
RCB1647 : RAJI update : 2021/04/08 |
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Comment | Burkitt's lymphoma. Sensitive to LAK cells but resistant to NK cells. Back up of culture ECA2673. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a 'non-producer'; the EBV genome carries deletions attributed to preventing the formation of virus particles. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 85011429 prior to order. | |||
Comment from the depositor | ||||
Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011429 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
Remarks | ||||
Order Form | Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | ECACC | ||
Year of deposit | 2000 | |||
Animal | _human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Gender | Male | |||
Age at sampling | 11 years | |||
Tissue | maxilla | |||
Disease name | Burkitt's lymphoma | |||
Classification | cancer | |||
History | overseas, ECACC -> RIKEN Cell bank | Lifespan | infinite | |
Morphology | lymphocyte-like | |||
Medium | Medium List | Culture type | Suspension cells | |
Medium and additives | RPMI1640 + 10% FBS | |||
Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 10 split |
SC frequency | Subculture : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
STR(human) | OK | |||
Virus (HIV) | LT 400 | |||
Virus (HTLV-1) | (-) | |||
Virus (EBV) | (+) | |||
Isozyme | LD, NP, AST | |||
Chromosome mode | 46-50(50) : 46(4),47(13),48(16),49(15),50(2) | |||
Reference information | Reference | 5 | ||
User's Publication | 14 | |||