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Cell No. : Cell Name
RCB1645 : Y79  update : 2024/01/17
CommentCaucasian retinoblastoma. May have reverse transcriptase. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 86093003 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86093003 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Year of deposit 2000
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 2.5 years
Tissue eye
Disease name retinoblastoma
Classification cancer
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1893
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 32-188(18) : /34(1),35(5),37(2),40(1),45(1),46(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 34

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4982  McFall RC, Nagy RM, Nagle BT, McGreevy LM.  Scanning electron microscopic observation of two retinoblastoma cell lines.  Cancer Res  1978  38(9):2827-35  PubMed ID: 679190  
4981  McFall RC, Sery TW, Makadon M.  Characterization of a new continuous cell line derived from a human retinoblastoma.  Cancer Res  1977  37(4):1003-10  PubMed ID: 844036  
1167  Reid, T W, Albert, D M, Rabson, A S, Russell P, Craft J, Chu, E W, Tralka, T S, Wilcox, J L  Characteristics of an established cell line of retinoblastoma.  J Natl Cancer Inst  1974  53:347-60  PubMed ID: 4135597   DOI: 10.1093/jnci/53.2.347

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User's Publication
18226  Maradani BS, Parameswaran S, Subramanian K.  Development and characterization of DNA aptamer against Retinoblastoma by Cell-SELEX  Sci Rep  2022  12(1):16178  PubMed ID: 36171412   DOI: 10.1038/s41598-022-20660-3
16736  Karmakar A, Ahamad Khan MM, Kumari N, Devarajan N, Ganesan SK.  Identification of Epigenetically Modified Hub Genes and Altered Pathways Associated With Retinoblastoma  Front Cell Dev Biol  2022  10:743224  PubMed ID: 35359459   DOI: 10.3389/fcell.2022.743224
17449  Nair RM, Revu NVL, Gali S, Kallamadi PR, Prabhu V, Manukonda R, Nemani H, Kaliki S, Vemuganti GK.  A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells  Indian J Ophthalmol  2022  70(5):1703-1711  PubMed ID: 35502056   DOI: 10.4103/ijo.IJO_2348_21
20394  Narayana RVL, Jana P, Tomar N, Prabhu V, Nair RM, Manukonda R, Kaliki S, Coupland SE, Alexander J, Kalirai H, Kondapi AK, Vemuganti GK.  Carboplatin- and Etoposide-Loaded Lactoferrin Protein Nanoparticles for Targeting Cancer Stem Cells in Retinoblastoma In Vitro.  Invest Ophthalmol Vis Sci  2021  62(14):13  PubMed ID: 34784412   DOI: 10.1167/iovs.62.14.13
10610  Kalmodia S, Parameswaran S, Ganapathy K, Yang W, Barrow CJ, Kanwar JR, Roy K, Vasudevan M, Kulkarni K, Elchuri SV, Krishnakumar S.  Characterization and Molecular Mechanism of Peptide-Conjugated Gold Nanoparticle Inhibiting p53-HDM2 Interaction in Retinoblastoma.  Mol Ther Nucleic Acids  2017  9:349-364  PubMed ID: 29246314   DOI: 10.1016/j.omtn.2017.10.012
14765  Nair RM, Balla MM, Khan I, Kalathur RKR, Kondaiah P, Vemuganti GK.  In vitro characterization of CD133 lo cancer stem cells in Retinoblastoma Y79 cell line  BMC Cancer  2017  17(1):779  PubMed ID: 29162051   DOI: 10.1186/s12885-017-3750-2
9644  Venkatesan N, Kanwar JR, Deepa PR, Navaneethakrishnan S, Joseph C, Krishnakumar S.  Targeting HSP90/Survivin using a cell permeable structure based peptido-mimetic shepherdin in retinoblastoma.  Chem. Biol. Interact.  2016  252:141-9  PubMed ID: 27062892   DOI: 10.1016/j.cbi.2016.04.011
9743  Nalini V, Deepa PR, Raguraman R, Khetan V, Reddy MA, Krishnakumar S.  Targeting HMGA2 in Retinoblastoma Cells in vitro Using the Aptamer Strategy.  Ocul Oncol Pathol  2016  2:262-269  PubMed ID: 27843907   DOI: 10.1159/000447300
9903  Danda R, Ganapathy K, Sathe G, Madugundu AK, Ramachandran S, Krishnan UM, Khetan V, Rishi P, Keshava Prasad TS, Pandey A, Krishnakumar S, Gowda H, Elchuri SV.  Proteomic profiling of retinoblastoma by high resolution mass spectrometry.  Clin Proteomics  2016  13:29  PubMed ID: 27799869   DOI: 10.1186/s12014-016-9128-7
10861    Targeting CD44, ABCG2 and CD133 markers using aptamers: in silico analysis of CD133 extracellular domain 2 and its aptamer  RSC Adv.  2016  6, 32115-32123    DOI: 10.1039/C5RA27072C
12953  Kalmodia S, Vandhana S, Tejaswini Rama BR, Jayashree B, Sreenivasan Seethalakshmi T, Umashankar V, Yang W, Barrow CJ, Krishnakumar S, Elchuri SV.  Bio-conjugation of antioxidant peptide on surface-modified gold nanoparticles: a novel approach to enhance the radical scavenging property in cancer cell  Cancer Nanotechnol  2016  7:1  PubMed ID: 26900409   DOI: 10.1186/s12645-016-0013-x
14153  Subramanian N, Srimany A, Kanwar JR, Kanwar RK, Akilandeswari B, Rishi P, Khetan V, Vasudevan M, Pradeep T, Krishnakumar S.  Nucleolin-aptamer therapy in retinoblastoma: molecular changes and mass spectrometry-based imaging  Mol Ther Nucleic Acids  2016  5(8):e358  PubMed ID: 27574784   DOI: 10.1038/mtna.2016.70
7342  Venkatesan N, Deepa PR, Khetan V, Krishnakumar S.  Computational and in vitro Investigation of miRNA-Gene Regulations in Retinoblastoma Pathogenesis: miRNA Mimics Strategy.  Bioinform Biol Insights  2015  9:89-101  PubMed ID: 25983556   DOI: 10.4137/BBI.S21742
7360  Sangeetha M, Deepa PR, Rishi P, Khetan V, Krishnakumar S.  Global gene deregulations in FASN silenced retinoblastoma cancer cells: molecular and clinico-pathological correlations.  J. Cell. Biochem.  2015  116:2676-94  PubMed ID: 25958981   DOI: 10.1002/jcb.25217
9419  Tatsuya Yunoki, Yoshiaki Tabuchi, Atsushi Hayashi, Takashi Kondo  BAG3 Protects Against Hyperthermic Stress by Modulating NF-κB and ERK Activities in Human Retinoblastoma Cells  Graefes Arch Clin Exp Ophthalmol  2015  253(3):399-407  PubMed ID: 25471019   DOI: 10.1007/s00417-014-2874-1
14313  Subramanian N, Kanwar JR, Kanwar RK, Krishnakumar S.  Blocking the maturation of OncomiRNAs using pri-miRNA-17∼92 aptamer in retinoblastoma  Nucleic Acid Ther  2015  25(1):47-52  PubMed ID: 25513843   DOI: 10.1089/nat.2014.0507
14389  Sreenivasan S, Krishnakumar S.  Synergistic Effect of Curcumin in Combination with Anticancer Agents in Human Retinoblastoma Cancer Cell Lines  Curr Eye Res  2015  40(11):1153-65  PubMed ID: 25495096   DOI: 10.3109/02713683.2014.987870
15237  Srimany A, Jayashree B, Krishnakumar S, Elchuri S, Pradeep T.  Identification of effective substrates for the direct analysis of lipids from cell lines using desorption electrospray ionization mass spectrometry  Rapid Commun Mass Spectrom  2015  29(4):349-56  PubMed ID: 26406347   DOI: 10.1002/rcm.7111
17447  Beta M, Chitipothu S, Khetan V, Biswas J, Krishnakumar S.  Hypermethylation of adenomatosis polyposis coli-2 and its tumor suppressor role in retinoblastoma  Curr Eye Res  2015  40(7):719-28  PubMed ID: 25207834   DOI: 10.3109/02713683.2014.954673
6000  Razak SR, Ueno K, Takayama N, Nariai N, Nagasaki M, Saito R, Koso H, Lai CY, Murakami M, Tsuji K, Michiue T, Nakauchi H, Otsu M, Watanabe S.  Profiling of microRNA in human and mouse ES and iPS cells reveals overlapping but distinct microRNA expression patterns.  PLoS One  2013  8(9):e73532  PubMed ID: 24086284   DOI: 10.1371/journal.pone.0073532
17448  Mitra M, Kandalam M, Rangasamy J, Shankar B, Maheswari UK, Swaminathan S, Krishnakumar S.  Novel epithelial cell adhesion molecule antibody conjugated polyethyleneimine-capped gold nanoparticles for enhanced and targeted small interfering RNA delivery to retinoblastoma cells  Mol Vis  2013  19:1029-38  PubMed ID: 23687439  
7685  Saito R, Nakauchi H, Watanabe S.  Serine/threonine kinase, Melk, regulates proliferation and glial differentiation of retinal progenitor cells.  Cancer Sci.  2012  103:42-9  PubMed ID: 21923749   DOI: 10.1111/j.1349-7006.2011.02104.x
13724  Vandhana S, Lakshmi TS, Indra D, Deepa PR, Krishnakumar S.  Microarray analysis and biochemical correlations of oxidative stress responsive genes in retinoblastoma  Curr Eye Res  2012  37(9):830-41  PubMed ID: 22668346   DOI: 10.3109/02713683.2012.678544
13761  Venkatesan N, Krishnakumar S, Deepa PR, Deepa M, Khetan V, Reddy MA.  Molecular deregulation induced by silencing of the high mobility group protein A2 gene in retinoblastoma cells  Mol Vis  2012  18:2420-37  PubMed ID: 23077401  
14407  Mitra M, Mohanty C, Harilal A, Maheswari UK, Sahoo SK, Krishnakumar S.  A novel in vitro three-dimensional retinoblastoma model for evaluating chemotherapeutic drugs  Mol Vis  2012  18:1361-78  PubMed ID: 22690114  
7883  Baba Y, Iida A, Watanabe S.  Sall3 plays essential roles in horizontal cell maturation through regulation of neurofilament expression levels.  Biochimie  2011  93:1037-46  PubMed ID: 21396426   DOI: 10.1016/j.biochi.2011.02.016
8346  Ishikawa Y, Nagai J, Okada Y, Sato K, Yumoto R, Takano M.  Function and expression of ATP-binding cassette transporters in cultured human Y79 retinoblastoma cells.  Biol. Pharm. Bull.  2010  33:504-11  PubMed ID: 20190417   DOI: 10.1248/bpb.33.504
8491  Inoue M, Iida A, Satoh S, Kodama T, Watanabe S.  COUP-TFI and -TFII nuclear receptors are expressed in amacrine cells and play roles in regulating the differentiation of retinal progenitor cells.  Exp. Eye Res.  2010  90:49-56  PubMed ID: 19766631   DOI: 10.1016/j.exer.2009.09.009
8478  Satoh S, Tang K, Iida A, Inoue M, Kodama T, Tsai SY, Tsai MJ, Furuta Y, Watanabe S.  The spatial patterning of mouse cone opsin expression is regulated by bone morphogenetic protein signaling through downstream effector COUP-TF nuclear receptors.  J. Neurosci.  2009  29:12401-11  PubMed ID: 19812316   DOI: 10.1523/JNEUROSCI.0951-09.2009
8620  Mikami-Terao Y, Akiyama M, Yuza Y, Yanagisawa T, Yamada O, Kawano T, Agawa M, Ida H, Yamada H.  Antitumor activity of TMPyP4 interacting G-quadruplex in retinoblastoma cell lines.  Exp. Eye Res.  2009  89:200-8  PubMed ID: 19328784   DOI: 10.1016/j.exer.2009.03.008
8733  Lin YP, Ouchi Y, Satoh S, Watanabe S.  Sox2 plays a role in the induction of amacrine and Muller glial cells in mouse retinal progenitor cells.  Invest. Ophthalmol. Vis. Sci.  2009  50:68-74  PubMed ID: 18719084   DOI: 10.1167/iovs.07-1619
2660  Hayakawa, Kou, Guo, Lei, Terentyeva, Elena A, Li, Xiao Kang, Kimura, Hiromitsu, Hirano, Masahiko, Yoshikawa, Kazuyuki, Yoshinaga, Teruo, Nagamine, Takeaki, Katsumata, Noriyuki, Tanaka, Toshiaki  Size-exclusion chromatography of biological samples which contain extremely alkaline proteins.  J Biochem Biophys Methods  2003  56:153-63  PubMed ID: 12834974   DOI: 10.1016/s0165-022x(03)00056-3
15611  Udono T, Takahashi K, Yasumoto K, Yoshizawa M, Takeda K, Abe T, Tamai M, Shibahara S.  Expression of tyrosinase-related protein 2/DOPAchrome tautomerase in the retinoblastoma  Exp Eye Res  2001  72(3):225-34  PubMed ID: 11180971   DOI: 10.1006/exer.2000.0948
1971  Kato G, Maeda S  Novel phosphorylation at a mitotic site, serine 75, in human pp60c-src from unsynchronized human tumor cells having a spherical morphology.  Biochem Biophys Res Commun  1995  216:619-29  PubMed ID: 7488156   DOI: 10.1006/bbrc.1995.2667

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