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Cell No. : Cell Name
RCB1645 : Y79  update : 2021/05/20
CommentCaucasian retinoblastoma. May have reverse transcriptase. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 86093003 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86093003 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Year of deposit 2000
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 2.5 years
Tissue eye
Disease name retinoblastoma
Classification cancer
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_1893
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 32-188(18) : /34(1),35(5),37(2),40(1),45(1),46(1)/
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 30

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4982  McFall RC, Nagy RM, Nagle BT, McGreevy LM.  Scanning electron microscopic observation of two retinoblastoma cell lines.  Cancer Res  1978  38(9):2827-35  PubMed ID: 679190
4981  McFall RC, Sery TW, Makadon M.  Characterization of a new continuous cell line derived from a human retinoblastoma.  Cancer Res  1977  37(4):1003-10  PubMed ID: 844036
1167  Reid, T W, Albert, D M, Rabson, A S, Russell P, Craft J, Chu, E W, Tralka, T S, Wilcox, J L  Characteristics of an established cell line of retinoblastoma.  J Natl Cancer Inst  1974  53:347-60  PubMed ID: 4135597

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User's Publication
20394  Narayana RVL, Jana P, Tomar N, Prabhu V, Nair RM, Manukonda R, Kaliki S, Coupland SE, Alexander J, Kalirai H, Kondapi AK, Vemuganti GK.  Carboplatin- and Etoposide-Loaded Lactoferrin Protein Nanoparticles for Targeting Cancer Stem Cells in Retinoblastoma In Vitro.  Invest Ophthalmol Vis Sci  2021  62(14):13  PubMed ID: 34784412
10610  Kalmodia S, Parameswaran S, Ganapathy K, Yang W, Barrow CJ, Kanwar JR, Roy K, Vasudevan M, Kulkarni K, Elchuri SV, Krishnakumar S.  Characterization and Molecular Mechanism of Peptide-Conjugated Gold Nanoparticle Inhibiting p53-HDM2 Interaction in Retinoblastoma.  Mol Ther Nucleic Acids  2017  9:349-364  PubMed ID: 29246314
14765  Nair RM, Balla MM, Khan I, Kalathur RKR, Kondaiah P, Vemuganti GK.  In vitro characterization of CD133 lo cancer stem cells in Retinoblastoma Y79 cell line  BMC Cancer  2017  17(1):779  PubMed ID: 29162051
9644  Venkatesan N, Kanwar JR, Deepa PR, Navaneethakrishnan S, Joseph C, Krishnakumar S.  Targeting HSP90/Survivin using a cell permeable structure based peptido-mimetic shepherdin in retinoblastoma.  Chem. Biol. Interact.  2016  252:141-9  PubMed ID: 27062892
9743  Nalini V, Deepa PR, Raguraman R, Khetan V, Reddy MA, Krishnakumar S.  Targeting HMGA2 in Retinoblastoma Cells in vitro Using the Aptamer Strategy.  Ocul Oncol Pathol  2016  2:262-269  PubMed ID: 27843907
9903  Danda R, Ganapathy K, Sathe G, Madugundu AK, Ramachandran S, Krishnan UM, Khetan V, Rishi P, Keshava Prasad TS, Pandey A, Krishnakumar S, Gowda H, Elchuri SV.  Proteomic profiling of retinoblastoma by high resolution mass spectrometry.  Clin Proteomics  2016  13:29  PubMed ID: 27799869
10861    Targeting CD44, ABCG2 and CD133 markers using aptamers: in silico analysis of CD133 extracellular domain 2 and its aptamer  RSC Adv.  2016  6, 32115-32123 
12953  Kalmodia S, Vandhana S, Tejaswini Rama BR, Jayashree B, Sreenivasan Seethalakshmi T, Umashankar V, Yang W, Barrow CJ, Krishnakumar S, Elchuri SV.  Bio-conjugation of antioxidant peptide on surface-modified gold nanoparticles: a novel approach to enhance the radical scavenging property in cancer cell  Cancer Nanotechnol  2016  7:1  PubMed ID: 26900409
14153  Subramanian N, Srimany A, Kanwar JR, Kanwar RK, Akilandeswari B, Rishi P, Khetan V, Vasudevan M, Pradeep T, Krishnakumar S.  Nucleolin-aptamer therapy in retinoblastoma: molecular changes and mass spectrometry-based imaging  Mol Ther Nucleic Acids  2016  5(8):e358  PubMed ID: 27574784
7342  Venkatesan N, Deepa PR, Khetan V, Krishnakumar S.  Computational and in vitro Investigation of miRNA-Gene Regulations in Retinoblastoma Pathogenesis: miRNA Mimics Strategy.  Bioinform Biol Insights  2015  9:89-101  PubMed ID: 25983556
7360  Sangeetha M, Deepa PR, Rishi P, Khetan V, Krishnakumar S.  Global gene deregulations in FASN silenced retinoblastoma cancer cells: molecular and clinico-pathological correlations.  J. Cell. Biochem.  2015  116:2676-94  PubMed ID: 25958981
9419  Tatsuya Yunoki, Yoshiaki Tabuchi, Atsushi Hayashi, Takashi Kondo  BAG3 Protects Against Hyperthermic Stress by Modulating NF-κB and ERK Activities in Human Retinoblastoma Cells  Graefes Arch Clin Exp Ophthalmol  2015  253(3):399-407  PubMed ID: 25471019
14313  Subramanian N, Kanwar JR, Kanwar RK, Krishnakumar S.  Blocking the maturation of OncomiRNAs using pri-miRNA-17∼92 aptamer in retinoblastoma  Nucleic Acid Ther  2015  25(1):47-52  PubMed ID: 25513843
14389  Sreenivasan S, Krishnakumar S.  Synergistic Effect of Curcumin in Combination with Anticancer Agents in Human Retinoblastoma Cancer Cell Lines  Curr Eye Res  2015  40(11):1153-65  PubMed ID: 25495096
15237  Srimany A, Jayashree B, Krishnakumar S, Elchuri S, Pradeep T.  Identification of effective substrates for the direct analysis of lipids from cell lines using desorption electrospray ionization mass spectrometry  Rapid Commun Mass Spectrom  2015  29(4):349-56  PubMed ID: 26406347
6000  Razak SR, Ueno K, Takayama N, Nariai N, Nagasaki M, Saito R, Koso H, Lai CY, Murakami M, Tsuji K, Michiue T, Nakauchi H, Otsu M, Watanabe S.  Profiling of microRNA in human and mouse ES and iPS cells reveals overlapping but distinct microRNA expression patterns.  PLoS One  2013  8(9):e73532  PubMed ID: 24086284
7685  Saito R, Nakauchi H, Watanabe S.  Serine/threonine kinase, Melk, regulates proliferation and glial differentiation of retinal progenitor cells.  Cancer Sci.  2012  103:42-9  PubMed ID: 21923749
13724  Vandhana S, Lakshmi TS, Indra D, Deepa PR, Krishnakumar S.  Microarray analysis and biochemical correlations of oxidative stress responsive genes in retinoblastoma  Curr Eye Res  2012  37(9):830-41  PubMed ID: 22668346
13761  Venkatesan N, Krishnakumar S, Deepa PR, Deepa M, Khetan V, Reddy MA.  Molecular deregulation induced by silencing of the high mobility group protein A2 gene in retinoblastoma cells  Mol Vis  2012  18:2420-37  PubMed ID: 23077401
14407  Mitra M, Mohanty C, Harilal A, Maheswari UK, Sahoo SK, Krishnakumar S.  A novel in vitro three-dimensional retinoblastoma model for evaluating chemotherapeutic drugs  Mol Vis  2012  18:1361-78  PubMed ID: 22690114
7883  Baba Y, Iida A, Watanabe S.  Sall3 plays essential roles in horizontal cell maturation through regulation of neurofilament expression levels.  Biochimie  2011  93:1037-46  PubMed ID: 21396426
8346  Ishikawa Y, Nagai J, Okada Y, Sato K, Yumoto R, Takano M.  Function and expression of ATP-binding cassette transporters in cultured human Y79 retinoblastoma cells.  Biol. Pharm. Bull.  2010  33:504-11  PubMed ID: 20190417
8491  Inoue M, Iida A, Satoh S, Kodama T, Watanabe S.  COUP-TFI and -TFII nuclear receptors are expressed in amacrine cells and play roles in regulating the differentiation of retinal progenitor cells.  Exp. Eye Res.  2010  90:49-56  PubMed ID: 19766631
8478  Satoh S, Tang K, Iida A, Inoue M, Kodama T, Tsai SY, Tsai MJ, Furuta Y, Watanabe S.  The spatial patterning of mouse cone opsin expression is regulated by bone morphogenetic protein signaling through downstream effector COUP-TF nuclear receptors.  J. Neurosci.  2009  29:12401-11  PubMed ID: 19812316
8620  Mikami-Terao Y, Akiyama M, Yuza Y, Yanagisawa T, Yamada O, Kawano T, Agawa M, Ida H, Yamada H.  Antitumor activity of TMPyP4 interacting G-quadruplex in retinoblastoma cell lines.  Exp. Eye Res.  2009  89:200-8  PubMed ID: 19328784
8733  Lin YP, Ouchi Y, Satoh S, Watanabe S.  Sox2 plays a role in the induction of amacrine and Muller glial cells in mouse retinal progenitor cells.  Invest. Ophthalmol. Vis. Sci.  2009  50:68-74  PubMed ID: 18719084
2660  Hayakawa, Kou, Guo, Lei, Terentyeva, Elena A, Li, Xiao Kang, Kimura, Hiromitsu, Hirano, Masahiko, Yoshikawa, Kazuyuki, Yoshinaga, Teruo, Nagamine, Takeaki, Katsumata, Noriyuki, Tanaka, Toshiaki  Size-exclusion chromatography of biological samples which contain extremely alkaline proteins.  J Biochem Biophys Methods  2003  56:153-63  PubMed ID: 12834974
15611  Udono T, Takahashi K, Yasumoto K, Yoshizawa M, Takeda K, Abe T, Tamai M, Shibahara S.  Expression of tyrosinase-related protein 2/DOPAchrome tautomerase in the retinoblastoma  Exp Eye Res  2001  72(3):225-34  PubMed ID: 11180971
2363  Hayashi N, Ido E, Ohtsuki Y, Ueno H  An experimental application of gene therapy for human retinoblastoma.  Invest Ophthalmol Vis Sci  1999  40:265-72  PubMed ID: 9950583
1971  Kato G, Maeda S  Novel phosphorylation at a mitotic site, serine 75, in human pp60c-src from unsynchronized human tumor cells having a spherical morphology.  Biochem Biophys Res Commun  1995  216:619-29  PubMed ID: 7488156

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