細胞番号 : 細胞名
RCB1644 : BeWo
update : 2024/08/14
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| 細胞特性(Comment:英) | It was initiated from a malignant gestational choriocarcinoma of the fetal placenta. Need to check response to hormones before use. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 86082803 prior to order. |
| 細胞特性(日) | 胎児胎盤由来の悪性の妊娠性絨毛癌由来細胞株。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、ECACC 86082803 を必ずご参照ください。BeWoの性別について。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86082803 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、ECA86082803 を必ず参照すること。2) 商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
ECACC
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| 樹立者 |
Pattillo, R.A. and Gey, G.O.
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| 寄託日 |
2000
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 採取組織 |
fetal placenta
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| 病名 |
choriocarcinoma
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| 細胞分類 |
cancer
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| 入手歴 |
overseas, ECACC -> RIKEN Cell bank
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細胞寿命 |
infinite
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| 細胞形態 |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_0044
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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HamF12 + 10% FBS
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| 抗生物質 |
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Free
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継代方法 |
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(0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
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継代密度 |
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1 : 4 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 染色体検査 |
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76-83(50) : /78(3),79(12),80(15),81(12),82(5),83(1)
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| 個体識別検査 |
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OK
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| 文献情報 |
Reference(英) |
5件
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| Reference(日) |
1件
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| 利用者成果(英) |
19件
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| 利用者成果(日) |
0件
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| 利用者成果(英) |
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21619
Noguchi S, Tozawa S, Sakurai T, Ohkuchi A, Takahashi H, Fujiwara H, Takizawa T.
BeWo exomeres are enriched for bioactive extracellular placenta-specific C19MC miRNAs.
J Reprod Immunol
2024
161:104187
PubMed ID: 38199177
DOI: 10.1016/j.jri.2023.104187
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20802
Ogushi S, Nakanishi T, Kimura T.
Cadmium inhibits forskolin-induced differentiation of human placental BeWo cells.
J Toxicol Sci
2022
47(8)
PubMed ID: 35908931
DOI: 10.2131/jts.47.309
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20966
Akino S, Yasujima T, Yamashiro T, Yuasa H.
Disrupted in renal carcinoma 2 (DIRC2/SLC49A4) is an H+-driven lysosomal pyridoxine exporter.
Life Sci Alliance
2022
6(2)
PubMed ID: 36456177
DOI: 10.26508/lsa.202201629
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13439
KanaeSakai, Shigenori Miura, Jun Sawayama, Shoji Takeuchi
Membrane-integrated glass chip for two-directional observation of epithelial cells
Sensors and Actuators B: Chemical
2021
DOI: 10.1016/j.snb.2020.128861
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12302
Zhuosi Li, Osamu Kurosawa, Hiroo Iwata
A comparative study of key physiological stem cell parameters between three human trophoblast cell lines
Biochem Biophys Res Commun
2020
525(4):1038-1045
PubMed ID: 32178868
DOI: 10.1016/j.bbrc.2020.03.031
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14664
Takahashi H, Ohkuchi A, Kuwata T, Usui R, Baba Y, Suzuki H, Chaw Kyi TT, Matsubara S, Saito S, Takizawa T.
Endogenous and exogenous miR-520c-3p modulates CD44-mediated extravillous trophoblast invasion
Placenta
2017
50:25-31
PubMed ID: 28161058
DOI: 10.1016/j.placenta.2016.12.016
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7152
Miura S, Sato K, Kato-Negishi M, Teshima T, Takeuchi S.
Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6.
Nat Commun
2015
6:8871
PubMed ID: 26563429
DOI: 10.1038/ncomms9871
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15794
Matsumoto J, Ariyoshi N, Ishii I, Kitada M.
Functional characterization of seven single-nucleotide polymorphisms of the steroid sulfatase gene found in a Japanese population
J Hum Genet
2013
58(5):267-72
PubMed ID: 23466819
DOI: 10.1038/jhg.2013.12
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17187
Ying Li
Aggregatibacter Actinomycetemcomitans reduced GNAS Gene Expression in Human Trophoblasts
Journal of Hard Tissue Biology
2013
22:35-40
DOI: 10.2485/jhtb.22.35
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5414
Takai N, Kira N, Ishii T, Yoshida T, Nishida M, Nishida Y, Nasu K, Takano M, Midori H, Koga S, Narahara H.
A translocator protein ligand PK11195 shows antigrowth activity in human choriocarcinoma cells.
Tumour Biol
2012
33(5):1505-10
PubMed ID: 22528948
DOI: 10.1007/s13277-012-0401-8
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7799
Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nishida Y, Nasu K, Narahara H.
Effects of bufalin on the proliferation of human choriocarcinoma cells.
Int. J. Gynecol. Cancer
2011
21:1105-9
PubMed ID: 21633303
DOI: 10.1097/IGC.0b013e318218730e
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8005
Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nasu K, Narahara H.
Erucylphosphocholine induces growth inhibition, cell cycle arrest, and apoptosis in human choriocarcinoma cells.
Tumour Biol.
2011
32:569-74
PubMed ID: 21213097
DOI: 10.1007/s13277-010-0152-3
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8055
Li Y, Shibata Y, Zhang L, Kuboyama N, Abiko Y.
Periodontal pathogen Aggregatibacter actinomycetemcomitans LPS induces mitochondria-dependent-apoptosis in human placental trophoblasts.
Placenta
2011
32:11-9
PubMed ID: 21074850
DOI: 10.1016/j.placenta.2010.10.007
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18914
Takai N, Ueda T, Nishida M, Nasu K, Narahara H.
Histone deacetylase inhibitors induce growth inhibition, cell cycle arrest and apoptosis in human choriocarcinoma cells
Int J Mol Med
2008
21(1):109-15
PubMed ID: 18097623
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12782
Hirano T, Yasuda S, Osaka Y, Asari M, Kobayashi M, Itagaki S, Iseki K.
The inhibitory effects of fluoroquinolones on L-carnitine transport in placental cell line BeWo
Int J Pharm
2008
351(1-2):113-8
PubMed ID: 17977676
DOI: 10.1016/j.ijpharm.2007.09.022
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12789
Yasuda S, Hasui S, Kobayashi M, Itagaki S, Hirano T, Iseki K.
The mechanism of carrier-mediated transport of folates in BeWo cells: the involvement of heme carrier protein 1 in placental folate transport
Biosci Biotechnol Biochem
2008
72(2):329-34
PubMed ID: 18256483
DOI: 10.1271/bbb.70347
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8848
Yamamoto T, Kuniki K, Takekuma Y, Hirano T, Iseki K, Sugawara M.
Ribavirin uptake by cultured human choriocarcinoma (BeWo) cells and Xenopus laevis oocytes expressing recombinant plasma membrane human nucleoside transporters.
Eur. J. Pharmacol.
2007
557:1-8
PubMed ID: 17140564
DOI: 10.1016/j.ejphar.2006.10.062
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2550
Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F
Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.
Biol Pharm Bull
2001
24:1258-62
PubMed ID: 11725959
DOI: 10.1248/bpb.24.1258
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1390
Morikawa O, Sakai N, Obara H, Saito N.
Effects of interferon-alpha, interferon-gamma and cAMP on the transcriptional regulation of the serotonin transporter
Eur J Pharmacol
1998
;349(2-3):317-24
PubMed ID: 9671113
DOI: 10.1016/s0014-2999(98)00187-3
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