CELL SEARCH SYSTEM -CELL BANK- (RIKEN BRC) [RCB1644 : BeWo]

細胞番号 : 細胞名 RCB1644 : BeWo update : 2023/06/30
細胞特性(Comment:英)			It was initiated from a malignant gestational choriocarcinoma of the fetal placenta. Need to check response to hormones before use. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 86082803 prior to order.
細胞特性(日)			胎児胎盤由来の悪性の妊娠性絨毛癌由来細胞株。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、ECACC 86082803 を必ずご参照ください。BeWoの性別について。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86082803 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、ECA86082803 を必ず参照すること。2) 商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf 同意書(非営利学術目的)C-0003.pdf 同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	ECACC
		樹立者	Pattillo, R.A. and Gey, G.O.
		寄託日	2000
		動物種	_human < Mammals
		属名	Homo
		種名	sapiens
		採取組織	fetal placenta
		病名	choriocarcinoma
		細胞分類	cancer
		入手歴	overseas, ECACC -> RIKEN Cell bank
		細胞寿命	infinite
		細胞形態	epithelial-like
		Cellosaurus(Expasy)	CVCL_0044
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Adherent cells
		培地		HamF12 + 10% FBS
		抗生物質		Free
		継代方法		(0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
		継代密度		1 : 4 split
		継代・培地交換頻度		Subculture : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ		(-)
		アイソザイム検査		LD, NP
		染色体検査		76-83(50) : /78(3),79(12),80(15),81(12),82(5),83(1)
		個体識別検査		OK
文献情報		Reference(英)	5件
		Reference(日)	1件
		利用者成果(英)	17件
		利用者成果(日)	0件

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Reference(英)

2756 Sheppard DM, Fisher RA, Lawler SD. Karyotypic analysis and chromosome polymorphisms in four choriocarcinoma cell lines. Cancer Genet Cytogenet 1985 16(3):251-8 PubMed ID: 4038916 DOI: 10.1016/0165-4608(85)90052-4

2755 Sasaki S, Katayama PK, Roesler M, Pattillo RA, Mattingly RF, Ohkawa K. Cytogenetic analysis of choriocarcinoma cell lines. Nihon Sanka Fujinka Gakkai Zasshi 1982 34(12):2253-6 PubMed ID: 7153601

2680 Pattillo RA, Gey GO. The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro. Cancer Res 1968 28(7):1231-6 PubMed ID: 4299001

4980 Pattillo RA, Gey GO, Delfs E, Mattingly RF. Human hormone production in vitro. Science 1968 159(3822):1467-9 PubMed ID: 5753554 DOI: 10.1126/science.159.3822.1467

4979 HERTZ R. Choriocarcinoma of women maintained in serial passage in hamster and rat. Proc Soc Exp Biol Med 1959 102:77-81 PubMed ID: 14401422 DOI: 10.3181/00379727-102-25149

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Reference(日)

2585 望月眞人、丸尾猛 ヒト絨毛癌:BeWo 生体の科学 1992 43:428-429

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利用者成果(英)

21619 Noguchi S, Tozawa S, Sakurai T, Ohkuchi A, Takahashi H, Fujiwara H, Takizawa T. BeWo exomeres are enriched for bioactive extracellular placenta-specific C19MC miRNAs. J Reprod Immunol 2024 161:104187 PubMed ID: 38199177 DOI: 10.1016/j.jri.2023.104187

20802 Ogushi S, Nakanishi T, Kimura T. Cadmium inhibits forskolin-induced differentiation of human placental BeWo cells. J Toxicol Sci 2022 47(8) PubMed ID: 35908931 DOI: 10.2131/jts.47.309

20966 Akino S, Yasujima T, Yamashiro T, Yuasa H. Disrupted in renal carcinoma 2 (DIRC2/SLC49A4) is an H+-driven lysosomal pyridoxine exporter. Life Sci Alliance 2022 6(2) PubMed ID: 36456177 DOI: 10.26508/lsa.202201629

13439 KanaeSakai, Shigenori Miura, Jun Sawayama, Shoji Takeuchi Membrane-integrated glass chip for two-directional observation of epithelial cells Sensors and Actuators B: Chemical 2021 DOI: 10.1016/j.snb.2020.128861

12302 Zhuosi Li, Osamu Kurosawa, Hiroo Iwata A comparative study of key physiological stem cell parameters between three human trophoblast cell lines Biochem Biophys Res Commun 2020 525(4):1038-1045 PubMed ID: 32178868 DOI: 10.1016/j.bbrc.2020.03.031

14664 Takahashi H, Ohkuchi A, Kuwata T, Usui R, Baba Y, Suzuki H, Chaw Kyi TT, Matsubara S, Saito S, Takizawa T. Endogenous and exogenous miR-520c-3p modulates CD44-mediated extravillous trophoblast invasion Placenta 2017 50:25-31 PubMed ID: 28161058 DOI: 10.1016/j.placenta.2016.12.016

7152 Miura S, Sato K, Kato-Negishi M, Teshima T, Takeuchi S. Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6. Nat Commun 2015 6:8871 PubMed ID: 26563429 DOI: 10.1038/ncomms9871

15794 Matsumoto J, Ariyoshi N, Ishii I, Kitada M. Functional characterization of seven single-nucleotide polymorphisms of the steroid sulfatase gene found in a Japanese population J Hum Genet 2013 58(5):267-72 PubMed ID: 23466819 DOI: 10.1038/jhg.2013.12

17187 Ying Li Aggregatibacter Actinomycetemcomitans reduced GNAS Gene Expression in Human Trophoblasts Journal of Hard Tissue Biology 2013 22:35-40 DOI: 10.2485/jhtb.22.35

5414 Takai N, Kira N, Ishii T, Yoshida T, Nishida M, Nishida Y, Nasu K, Takano M, Midori H, Koga S, Narahara H. A translocator protein ligand PK11195 shows antigrowth activity in human choriocarcinoma cells. Tumour Biol 2012 33(5):1505-10 PubMed ID: 22528948 DOI: 10.1007/s13277-012-0401-8

7799 Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nishida Y, Nasu K, Narahara H. Effects of bufalin on the proliferation of human choriocarcinoma cells. Int. J. Gynecol. Cancer 2011 21:1105-9 PubMed ID: 21633303 DOI: 10.1097/IGC.0b013e318218730e

8005 Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nasu K, Narahara H. Erucylphosphocholine induces growth inhibition, cell cycle arrest, and apoptosis in human choriocarcinoma cells. Tumour Biol. 2011 32:569-74 PubMed ID: 21213097 DOI: 10.1007/s13277-010-0152-3

8055 Li Y, Shibata Y, Zhang L, Kuboyama N, Abiko Y. Periodontal pathogen Aggregatibacter actinomycetemcomitans LPS induces mitochondria-dependent-apoptosis in human placental trophoblasts. Placenta 2011 32:11-9 PubMed ID: 21074850 DOI: 10.1016/j.placenta.2010.10.007

12782 Hirano T, Yasuda S, Osaka Y, Asari M, Kobayashi M, Itagaki S, Iseki K. The inhibitory effects of fluoroquinolones on L-carnitine transport in placental cell line BeWo Int J Pharm 2008 351(1-2):113-8 PubMed ID: 17977676 DOI: 10.1016/j.ijpharm.2007.09.022

12789 Yasuda S, Hasui S, Kobayashi M, Itagaki S, Hirano T, Iseki K. The mechanism of carrier-mediated transport of folates in BeWo cells: the involvement of heme carrier protein 1 in placental folate transport Biosci Biotechnol Biochem 2008 72(2):329-34 PubMed ID: 18256483 DOI: 10.1271/bbb.70347

8848 Yamamoto T, Kuniki K, Takekuma Y, Hirano T, Iseki K, Sugawara M. Ribavirin uptake by cultured human choriocarcinoma (BeWo) cells and Xenopus laevis oocytes expressing recombinant plasma membrane human nucleoside transporters. Eur. J. Pharmacol. 2007 557:1-8 PubMed ID: 17140564 DOI: 10.1016/j.ejphar.2006.10.062

2550 Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues. Biol Pharm Bull 2001 24:1258-62 PubMed ID: 11725959 DOI: 10.1248/bpb.24.1258

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