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細胞材料の再培養方法 ( HPS以外・ HPS )
細胞番号 : 細胞名
RCB1644 : BeWo  update : 2025/01/16
細胞特性(Comment:英)It was initiated from a malignant gestational choriocarcinoma of the fetal placenta. Need to check response to hormones before use. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 86082803 prior to order.
細胞特性(日)胎児胎盤由来の悪性の妊娠性絨毛癌由来細胞株。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、ECACC 86082803 を必ずご参照ください。BeWoの性別について。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86082803 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、ECA86082803 を必ず参照すること。2) 商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 ECACC
樹立者 Pattillo, R.A. and Gey, G.O.
寄託日 2000
動物種 _human < Mammals
属名 Homo
種名 sapiens
採取組織 fetal placenta
病名 choriocarcinoma
細胞分類 cancer
入手歴 overseas, ECACC -> RIKEN Cell bank
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_0044
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 HamF12 + 10% FBS
抗生物質 Free
継代方法 (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
継代密度 1 : 4 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ/アコレプラズマ (-)
アイソザイム検査 LD, NP
染色体検査 76-83(50) : /78(3),79(12),80(15),81(12),82(5),83(1)
個体識別検査 OK
文献情報 Reference(英) 5件
Reference(日) 1件
利用者成果(英) 23件
利用者成果(日) 0件

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Reference(英)
2756  Sheppard DM, Fisher RA, Lawler SD.  Karyotypic analysis and chromosome polymorphisms in four choriocarcinoma cell lines.  Cancer Genet Cytogenet  1985  16(3):251-8  PubMed ID: 4038916   DOI: 10.1016/0165-4608(85)90052-4
2755  Sasaki S, Katayama PK, Roesler M, Pattillo RA, Mattingly RF, Ohkawa K.  Cytogenetic analysis of choriocarcinoma cell lines.  Nihon Sanka Fujinka Gakkai Zasshi  1982  34(12):2253-6  PubMed ID: 7153601  
2680  Pattillo RA, Gey GO.  The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro.  Cancer Res  1968  28(7):1231-6  PubMed ID: 4299001  
4980  Pattillo RA, Gey GO, Delfs E, Mattingly RF.  Human hormone production in vitro.  Science  1968  159(3822):1467-9  PubMed ID: 5753554   DOI: 10.1126/science.159.3822.1467
4979  HERTZ R.  Choriocarcinoma of women maintained in serial passage in hamster and rat.  Proc Soc Exp Biol Med  1959  102:77-81  PubMed ID: 14401422   DOI: 10.3181/00379727-102-25149

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Reference(日)
2585  望月 眞人、丸尾 猛  ヒト絨毛癌:BeWo  生体の科学  1992  43:428-429   

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利用者成果(英)
21619  Noguchi S, Tozawa S, Sakurai T, Ohkuchi A, Takahashi H, Fujiwara H, Takizawa T.  BeWo exomeres are enriched for bioactive extracellular placenta-specific C19MC miRNAs.  J Reprod Immunol  2024  161:104187  PubMed ID: 38199177   DOI: 10.1016/j.jri.2023.104187
22446  Togo A, Mitsuzuka K, Hanawa S, Nakajima R, Izumi K, Sato K, Ishimoto H.  Downregulation of SPARC Expression Enhances the Fusion of BeWo Choriocarcinoma Cells  Reprod Sci  2024  31(8):2342-2353  PubMed ID: 38728000   DOI: 10.1007/s43032-024-01563-2
20802  Ogushi S, Nakanishi T, Kimura T.  Cadmium inhibits forskolin-induced differentiation of human placental BeWo cells.  J Toxicol Sci  2022  47(8)  PubMed ID: 35908931   DOI: 10.2131/jts.47.309
20966  Akino S, Yasujima T, Yamashiro T, Yuasa H.  Disrupted in renal carcinoma 2 (DIRC2/SLC49A4) is an H+-driven lysosomal pyridoxine exporter.  Life Sci Alliance  2022  6(2)  PubMed ID: 36456177   DOI: 10.26508/lsa.202201629
13439  KanaeSakai, Shigenori Miura, Jun Sawayama, Shoji Takeuchi  Membrane-integrated glass chip for two-directional observation of epithelial cells  Sensors and Actuators B: Chemical  2021      DOI: 10.1016/j.snb.2020.128861
12302  Zhuosi Li, Osamu Kurosawa, Hiroo Iwata  A comparative study of key physiological stem cell parameters between three human trophoblast cell lines  Biochem Biophys Res Commun  2020  525(4):1038-1045  PubMed ID: 32178868   DOI: 10.1016/j.bbrc.2020.03.031
14664  Takahashi H, Ohkuchi A, Kuwata T, Usui R, Baba Y, Suzuki H, Chaw Kyi TT, Matsubara S, Saito S, Takizawa T.  Endogenous and exogenous miR-520c-3p modulates CD44-mediated extravillous trophoblast invasion  Placenta  2017  50:25-31  PubMed ID: 28161058   DOI: 10.1016/j.placenta.2016.12.016
6403  Terasaka N, Futai K, Katoh T, Suga H.  A human microRNA precursor binding to folic acid discovered by small RNA transcriptomic SELEX.  RNA  2016  22(12):1918-1928  PubMed ID: 27852928   DOI: 10.1261/rna.057737.116
7152  Miura S, Sato K, Kato-Negishi M, Teshima T, Takeuchi S.  Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6.  Nat Commun  2015  6:8871  PubMed ID: 26563429   DOI: 10.1038/ncomms9871
5623  Kambe S, Yoshitake H, Yuge K, Ishida Y, Ali MM, Takizawa T, Kuwata T, Ohkuchi A, Matsubara S, Suzuki M, Takeshita T, Saito S, Takizawa T.  Human exosomal placenta-associated miR-517a-3p modulates the expression of PRKG1 mRNA in Jurkat cells  Biol Reprod  2014  91(5):129  PubMed ID: 25273530   DOI: 10.1095/biolreprod.114.121616
15794  Matsumoto J, Ariyoshi N, Ishii I, Kitada M.  Functional characterization of seven single-nucleotide polymorphisms of the steroid sulfatase gene found in a Japanese population  J Hum Genet  2013  58(5):267-72  PubMed ID: 23466819   DOI: 10.1038/jhg.2013.12
17187  Ying Li  Aggregatibacter Actinomycetemcomitans reduced GNAS Gene Expression in Human Trophoblasts  Journal of Hard Tissue Biology  2013  22:35-40    DOI: 10.2485/jhtb.22.35
2092  S. Miura, Y. Morimoto, S. Takeuchi  Multi-layered placental barrier structure integrated with microfluidic channels  2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS)  2013  257-258    DOI: 10.1109/memsys.2013.6474226
5414  Takai N, Kira N, Ishii T, Yoshida T, Nishida M, Nishida Y, Nasu K, Takano M, Midori H, Koga S, Narahara H.  A translocator protein ligand PK11195 shows antigrowth activity in human choriocarcinoma cells.  Tumour Biol  2012  33(5):1505-10  PubMed ID: 22528948   DOI: 10.1007/s13277-012-0401-8
7799  Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nishida Y, Nasu K, Narahara H.  Effects of bufalin on the proliferation of human choriocarcinoma cells.  Int. J. Gynecol. Cancer  2011  21:1105-9  PubMed ID: 21633303   DOI: 10.1097/IGC.0b013e318218730e
8005  Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nasu K, Narahara H.  Erucylphosphocholine induces growth inhibition, cell cycle arrest, and apoptosis in human choriocarcinoma cells.  Tumour Biol.  2011  32:569-74  PubMed ID: 21213097   DOI: 10.1007/s13277-010-0152-3
8055  Li Y, Shibata Y, Zhang L, Kuboyama N, Abiko Y.  Periodontal pathogen Aggregatibacter actinomycetemcomitans LPS induces mitochondria-dependent-apoptosis in human placental trophoblasts.  Placenta  2011  32:11-9  PubMed ID: 21074850   DOI: 10.1016/j.placenta.2010.10.007
18914  Takai N, Ueda T, Nishida M, Nasu K, Narahara H.  Histone deacetylase inhibitors induce growth inhibition, cell cycle arrest and apoptosis in human choriocarcinoma cells  Int J Mol Med  2008  21(1):109-15  PubMed ID: 18097623  
12782  Hirano T, Yasuda S, Osaka Y, Asari M, Kobayashi M, Itagaki S, Iseki K.  The inhibitory effects of fluoroquinolones on L-carnitine transport in placental cell line BeWo  Int J Pharm  2008  351(1-2):113-8  PubMed ID: 17977676   DOI: 10.1016/j.ijpharm.2007.09.022
12789  Yasuda S, Hasui S, Kobayashi M, Itagaki S, Hirano T, Iseki K.  The mechanism of carrier-mediated transport of folates in BeWo cells: the involvement of heme carrier protein 1 in placental folate transport  Biosci Biotechnol Biochem  2008  72(2):329-34  PubMed ID: 18256483   DOI: 10.1271/bbb.70347
8848  Yamamoto T, Kuniki K, Takekuma Y, Hirano T, Iseki K, Sugawara M.  Ribavirin uptake by cultured human choriocarcinoma (BeWo) cells and Xenopus laevis oocytes expressing recombinant plasma membrane human nucleoside transporters.  Eur. J. Pharmacol.  2007  557:1-8  PubMed ID: 17140564   DOI: 10.1016/j.ejphar.2006.10.062
2550  Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F  Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.  Biol Pharm Bull  2001  24:1258-62  PubMed ID: 11725959   DOI: 10.1248/bpb.24.1258
1390  Morikawa O, Sakai N, Obara H, Saito N.  Effects of interferon-alpha, interferon-gamma and cAMP on the transcriptional regulation of the serotonin transporter  Eur J Pharmacol  1998  ;349(2-3):317-24  PubMed ID: 9671113   DOI: 10.1016/s0014-2999(98)00187-3

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