Cell No. : Cell Name
RCB1644 : BeWo
update : 2023/06/30
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| Comment | It was initiated from a malignant gestational choriocarcinoma of the fetal placenta. Need to check response to hormones before use. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 86082803 prior to order. |
| Comment from the depositor | |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86082803 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
ECACC
|
| Originator |
Pattillo, R.A. and Gey, G.O.
|
| Year of deposit |
2000
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Tissue |
fetal placenta
|
| Disease name |
choriocarcinoma
|
| Classification |
cancer
|
| History |
overseas, ECACC -> RIKEN Cell bank
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_0044
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
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| Culture medium |
|
HamF12 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
(0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
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Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma |
|
(-)
|
| Isozyme |
|
LD, NP
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| Chromosome mode |
|
76-83(50) : /78(3),79(12),80(15),81(12),82(5),83(1)
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
5
|
| User's Publication |
16
|
| Reference |
|
2756
Sheppard DM, Fisher RA, Lawler SD.
Karyotypic analysis and chromosome polymorphisms in four choriocarcinoma cell lines.
Cancer Genet Cytogenet
1985
16(3):251-8
PubMed ID: 4038916
DOI: 10.1016/0165-4608(85)90052-4
|
|
2755
Sasaki S, Katayama PK, Roesler M, Pattillo RA, Mattingly RF, Ohkawa K.
Cytogenetic analysis of choriocarcinoma cell lines.
Nihon Sanka Fujinka Gakkai Zasshi
1982
34(12):2253-6
PubMed ID: 7153601
|
|
2680
Pattillo RA, Gey GO.
The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro.
Cancer Res
1968
28(7):1231-6
PubMed ID: 4299001
|
|
4980
Pattillo RA, Gey GO, Delfs E, Mattingly RF.
Human hormone production in vitro.
Science
1968
159(3822):1467-9
PubMed ID: 5753554
DOI: 10.1126/science.159.3822.1467
|
|
4979
HERTZ R.
Choriocarcinoma of women maintained in serial passage in hamster and rat.
Proc Soc Exp Biol Med
1959
102:77-81
PubMed ID: 14401422
DOI: 10.3181/00379727-102-25149
|
| User's Publication |
|
20802
Ogushi S, Nakanishi T, Kimura T.
Cadmium inhibits forskolin-induced differentiation of human placental BeWo cells.
J Toxicol Sci
2022
47(8)
PubMed ID: 35908931
DOI: 10.2131/jts.47.309
|
|
20966
Akino S, Yasujima T, Yamashiro T, Yuasa H.
Disrupted in renal carcinoma 2 (DIRC2/SLC49A4) is an H+-driven lysosomal pyridoxine exporter.
Life Sci Alliance
2022
6(2)
PubMed ID: 36456177
DOI: 10.26508/lsa.202201629
|
|
13439
KanaeSakai, Shigenori Miura, Jun Sawayama, Shoji Takeuchi
Membrane-integrated glass chip for two-directional observation of epithelial cells
Sensors and Actuators B: Chemical
2021
DOI: 10.1016/j.snb.2020.128861
|
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12302
Zhuosi Li, Osamu Kurosawa, Hiroo Iwata
A comparative study of key physiological stem cell parameters between three human trophoblast cell lines
Biochem Biophys Res Commun
2020
525(4):1038-1045
PubMed ID: 32178868
DOI: 10.1016/j.bbrc.2020.03.031
|
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14664
Takahashi H, Ohkuchi A, Kuwata T, Usui R, Baba Y, Suzuki H, Chaw Kyi TT, Matsubara S, Saito S, Takizawa T.
Endogenous and exogenous miR-520c-3p modulates CD44-mediated extravillous trophoblast invasion
Placenta
2017
50:25-31
PubMed ID: 28161058
DOI: 10.1016/j.placenta.2016.12.016
|
|
7152
Miura S, Sato K, Kato-Negishi M, Teshima T, Takeuchi S.
Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6.
Nat Commun
2015
6:8871
PubMed ID: 26563429
DOI: 10.1038/ncomms9871
|
|
15794
Matsumoto J, Ariyoshi N, Ishii I, Kitada M.
Functional characterization of seven single-nucleotide polymorphisms of the steroid sulfatase gene found in a Japanese population
J Hum Genet
2013
58(5):267-72
PubMed ID: 23466819
DOI: 10.1038/jhg.2013.12
|
|
17187
Ying Li
Aggregatibacter Actinomycetemcomitans reduced GNAS Gene Expression in Human Trophoblasts
Journal of Hard Tissue Biology
2013
22:35-40
DOI: 10.2485/jhtb.22.35
|
|
5414
Takai N, Kira N, Ishii T, Yoshida T, Nishida M, Nishida Y, Nasu K, Takano M, Midori H, Koga S, Narahara H.
A translocator protein ligand PK11195 shows antigrowth activity in human choriocarcinoma cells.
Tumour Biol
2012
33(5):1505-10
PubMed ID: 22528948
DOI: 10.1007/s13277-012-0401-8
|
|
7799
Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nishida Y, Nasu K, Narahara H.
Effects of bufalin on the proliferation of human choriocarcinoma cells.
Int. J. Gynecol. Cancer
2011
21:1105-9
PubMed ID: 21633303
DOI: 10.1097/IGC.0b013e318218730e
|
|
8005
Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nasu K, Narahara H.
Erucylphosphocholine induces growth inhibition, cell cycle arrest, and apoptosis in human choriocarcinoma cells.
Tumour Biol.
2011
32:569-74
PubMed ID: 21213097
DOI: 10.1007/s13277-010-0152-3
|
|
8055
Li Y, Shibata Y, Zhang L, Kuboyama N, Abiko Y.
Periodontal pathogen Aggregatibacter actinomycetemcomitans LPS induces mitochondria-dependent-apoptosis in human placental trophoblasts.
Placenta
2011
32:11-9
PubMed ID: 21074850
DOI: 10.1016/j.placenta.2010.10.007
|
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12782
Hirano T, Yasuda S, Osaka Y, Asari M, Kobayashi M, Itagaki S, Iseki K.
The inhibitory effects of fluoroquinolones on L-carnitine transport in placental cell line BeWo
Int J Pharm
2008
351(1-2):113-8
PubMed ID: 17977676
DOI: 10.1016/j.ijpharm.2007.09.022
|
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12789
Yasuda S, Hasui S, Kobayashi M, Itagaki S, Hirano T, Iseki K.
The mechanism of carrier-mediated transport of folates in BeWo cells: the involvement of heme carrier protein 1 in placental folate transport
Biosci Biotechnol Biochem
2008
72(2):329-34
PubMed ID: 18256483
DOI: 10.1271/bbb.70347
|
|
8848
Yamamoto T, Kuniki K, Takekuma Y, Hirano T, Iseki K, Sugawara M.
Ribavirin uptake by cultured human choriocarcinoma (BeWo) cells and Xenopus laevis oocytes expressing recombinant plasma membrane human nucleoside transporters.
Eur. J. Pharmacol.
2007
557:1-8
PubMed ID: 17140564
DOI: 10.1016/j.ejphar.2006.10.062
|
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2550
Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F
Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.
Biol Pharm Bull
2001
24:1258-62
PubMed ID: 11725959
DOI: 10.1248/bpb.24.1258
|
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