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Cell No. : Cell Name
RCB1644 : BeWo  update : 2021/02/26
CommentIt was initiated from a malignant gestational choriocarcinoma of the fetal placenta. Need to check response to hormones before use. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 86082803 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86082803 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue fetal placenta
Disease name choriocarcinoma
Classification cancer
History overseas, ECACC -> RIKEN Cell bank
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method (0.05% trypsin + 0.02% EDTA) or (0.25% trypsin + 0.02% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 76-83(50) : /78(3),79(12),80(15),81(12),82(5),83(1)
Images
deposit info
lot info
Reference information Reference 5
User's Publication 11


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Reference
2756  Sheppard DM, Fisher RA, Lawler SD.  Karyotypic analysis and chromosome polymorphisms in four choriocarcinoma cell lines.  Cancer Genet Cytogenet  1985  16(3):251-8  PubMed ID: 4038916
2755  Sasaki S, Katayama PK, Roesler M, Pattillo RA, Mattingly RF, Ohkawa K.  Cytogenetic analysis of choriocarcinoma cell lines.  Nihon Sanka Fujinka Gakkai Zasshi  1982  34(12):2253-6  PubMed ID: 7153601
2680  Pattillo RA, Gey GO.  The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro.  Cancer Res  1968  28(7):1231-6  PubMed ID: 4299001
4980  Pattillo RA, Gey GO, Delfs E, Mattingly RF.  Human hormone production in vitro.  Science  1968  159(3822):1467-9  PubMed ID: 5753554
4979  HERTZ R.  Choriocarcinoma of women maintained in serial passage in hamster and rat.  Proc Soc Exp Biol Med  1959  102:77-81  PubMed ID: 14401422

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User's Publication
12302  Zhuosi Li, Osamu Kurosawa, Hiroo Iwata  A comparative study of key physiological stem cell parameters between three human trophoblast cell lines  Biochem Biophys Res Commun  2020  525(4):1038-1045  PubMed ID: 32178868
4075  Masahiro SUZUKI, Masahito MIZUUCHI, Tsuyoshi BABA, Yuya FUJIBE, Yoshihiko INO, Shinichi ISHIOKA, and Tsuyoshi SAITO  The roles of metformin and pravastatin on placental endoplasmic reticulum stress and placental growth factor in human villous-Like trophoblastic BeWo cells  The Sapporo Medical Journal  2018  87:75-84 
7152  Miura S, Sato K, Kato-Negishi M, Teshima T, Takeuchi S.  Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6.  Nat Commun  2015  6:8871  PubMed ID: 26563429
5414  Takai N, Kira N, Ishii T, Yoshida T, Nishida M, Nishida Y, Nasu K, Takano M, Midori H, Koga S, Narahara H.  A translocator protein ligand PK11195 shows antigrowth activity in human choriocarcinoma cells.  Tumour Biol  2012  33(5):1505-10  PubMed ID: 22528948
7799  Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nishida Y, Nasu K, Narahara H.  Effects of bufalin on the proliferation of human choriocarcinoma cells.  Int. J. Gynecol. Cancer  2011  21:1105-9  PubMed ID: 21633303
8005  Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nasu K, Narahara H.  Erucylphosphocholine induces growth inhibition, cell cycle arrest, and apoptosis in human choriocarcinoma cells.  Tumour Biol.  2011  32:569-74  PubMed ID: 21213097
8055  Li Y, Shibata Y, Zhang L, Kuboyama N, Abiko Y.  Periodontal pathogen Aggregatibacter actinomycetemcomitans LPS induces mitochondria-dependent-apoptosis in human placental trophoblasts.  Placenta  2011  32:11-9  PubMed ID: 21074850
12782  Hirano T, Yasuda S, Osaka Y, Asari M, Kobayashi M, Itagaki S, Iseki K.  The inhibitory effects of fluoroquinolones on L-carnitine transport in placental cell line BeWo  Int J Pharm  2008  351(1-2):113-8  PubMed ID: 17977676
12789  Yasuda S, Hasui S, Kobayashi M, Itagaki S, Hirano T, Iseki K.  The mechanism of carrier-mediated transport of folates in BeWo cells: the involvement of heme carrier protein 1 in placental folate transport  Biosci Biotechnol Biochem  2008  72(2):329-34  PubMed ID: 18256483
8848  Yamamoto T, Kuniki K, Takekuma Y, Hirano T, Iseki K, Sugawara M.  Ribavirin uptake by cultured human choriocarcinoma (BeWo) cells and Xenopus laevis oocytes expressing recombinant plasma membrane human nucleoside transporters.  Eur. J. Pharmacol.  2007  557:1-8  PubMed ID: 17140564
2550  Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F  Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.  Biol Pharm Bull  2001  24:1258-62  PubMed ID: 11725959



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