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Cell No. : Cell Name
RCB1644 : BeWo  update : 2024/05/16
CommentIt was initiated from a malignant gestational choriocarcinoma of the fetal placenta. Need to check response to hormones before use. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 86082803 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA86082803 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Originator Pattillo, R.A. and Gey, G.O.
Year of deposit 2000
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue fetal placenta
Disease name choriocarcinoma
Classification cancer
History overseas, ECACC -> RIKEN Cell bank
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0044
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% FBS
Antibiotics Free
Passage method (0.05% Trypsin + 0.02% EDTA) or (0.25% Trypsin + 0.04% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 76-83(50) : /78(3),79(12),80(15),81(12),82(5),83(1)
STR(human) OK
Reference information Reference 5
User's Publication 17

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2756  Sheppard DM, Fisher RA, Lawler SD.  Karyotypic analysis and chromosome polymorphisms in four choriocarcinoma cell lines.  Cancer Genet Cytogenet  1985  16(3):251-8  PubMed ID: 4038916   DOI: 10.1016/0165-4608(85)90052-4
2755  Sasaki S, Katayama PK, Roesler M, Pattillo RA, Mattingly RF, Ohkawa K.  Cytogenetic analysis of choriocarcinoma cell lines.  Nihon Sanka Fujinka Gakkai Zasshi  1982  34(12):2253-6  PubMed ID: 7153601  
2680  Pattillo RA, Gey GO.  The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro.  Cancer Res  1968  28(7):1231-6  PubMed ID: 4299001  
4980  Pattillo RA, Gey GO, Delfs E, Mattingly RF.  Human hormone production in vitro.  Science  1968  159(3822):1467-9  PubMed ID: 5753554   DOI: 10.1126/science.159.3822.1467
4979  HERTZ R.  Choriocarcinoma of women maintained in serial passage in hamster and rat.  Proc Soc Exp Biol Med  1959  102:77-81  PubMed ID: 14401422   DOI: 10.3181/00379727-102-25149

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User's Publication
21619  Noguchi S, Tozawa S, Sakurai T, Ohkuchi A, Takahashi H, Fujiwara H, Takizawa T.  BeWo exomeres are enriched for bioactive extracellular placenta-specific C19MC miRNAs.  J Reprod Immunol  2024  161:104187  PubMed ID: 38199177   DOI: 10.1016/j.jri.2023.104187
20802  Ogushi S, Nakanishi T, Kimura T.  Cadmium inhibits forskolin-induced differentiation of human placental BeWo cells.  J Toxicol Sci  2022  47(8)  PubMed ID: 35908931   DOI: 10.2131/jts.47.309
20966  Akino S, Yasujima T, Yamashiro T, Yuasa H.  Disrupted in renal carcinoma 2 (DIRC2/SLC49A4) is an H+-driven lysosomal pyridoxine exporter.  Life Sci Alliance  2022  6(2)  PubMed ID: 36456177   DOI: 10.26508/lsa.202201629
13439  KanaeSakai, Shigenori Miura, Jun Sawayama, Shoji Takeuchi  Membrane-integrated glass chip for two-directional observation of epithelial cells  Sensors and Actuators B: Chemical  2021      DOI: 10.1016/j.snb.2020.128861
12302  Zhuosi Li, Osamu Kurosawa, Hiroo Iwata  A comparative study of key physiological stem cell parameters between three human trophoblast cell lines  Biochem Biophys Res Commun  2020  525(4):1038-1045  PubMed ID: 32178868   DOI: 10.1016/j.bbrc.2020.03.031
14664  Takahashi H, Ohkuchi A, Kuwata T, Usui R, Baba Y, Suzuki H, Chaw Kyi TT, Matsubara S, Saito S, Takizawa T.  Endogenous and exogenous miR-520c-3p modulates CD44-mediated extravillous trophoblast invasion  Placenta  2017  50:25-31  PubMed ID: 28161058   DOI: 10.1016/j.placenta.2016.12.016
7152  Miura S, Sato K, Kato-Negishi M, Teshima T, Takeuchi S.  Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6.  Nat Commun  2015  6:8871  PubMed ID: 26563429   DOI: 10.1038/ncomms9871
15794  Matsumoto J, Ariyoshi N, Ishii I, Kitada M.  Functional characterization of seven single-nucleotide polymorphisms of the steroid sulfatase gene found in a Japanese population  J Hum Genet  2013  58(5):267-72  PubMed ID: 23466819   DOI: 10.1038/jhg.2013.12
17187  Ying Li  Aggregatibacter Actinomycetemcomitans reduced GNAS Gene Expression in Human Trophoblasts  Journal of Hard Tissue Biology  2013  22:35-40    DOI: 10.2485/jhtb.22.35
5414  Takai N, Kira N, Ishii T, Yoshida T, Nishida M, Nishida Y, Nasu K, Takano M, Midori H, Koga S, Narahara H.  A translocator protein ligand PK11195 shows antigrowth activity in human choriocarcinoma cells.  Tumour Biol  2012  33(5):1505-10  PubMed ID: 22528948   DOI: 10.1007/s13277-012-0401-8
7799  Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nishida Y, Nasu K, Narahara H.  Effects of bufalin on the proliferation of human choriocarcinoma cells.  Int. J. Gynecol. Cancer  2011  21:1105-9  PubMed ID: 21633303   DOI: 10.1097/IGC.0b013e318218730e
8005  Takai N, Ueda T, Ishii T, Kira N, Nishida M, Nasu K, Narahara H.  Erucylphosphocholine induces growth inhibition, cell cycle arrest, and apoptosis in human choriocarcinoma cells.  Tumour Biol.  2011  32:569-74  PubMed ID: 21213097   DOI: 10.1007/s13277-010-0152-3
8055  Li Y, Shibata Y, Zhang L, Kuboyama N, Abiko Y.  Periodontal pathogen Aggregatibacter actinomycetemcomitans LPS induces mitochondria-dependent-apoptosis in human placental trophoblasts.  Placenta  2011  32:11-9  PubMed ID: 21074850   DOI: 10.1016/j.placenta.2010.10.007
12782  Hirano T, Yasuda S, Osaka Y, Asari M, Kobayashi M, Itagaki S, Iseki K.  The inhibitory effects of fluoroquinolones on L-carnitine transport in placental cell line BeWo  Int J Pharm  2008  351(1-2):113-8  PubMed ID: 17977676   DOI: 10.1016/j.ijpharm.2007.09.022
12789  Yasuda S, Hasui S, Kobayashi M, Itagaki S, Hirano T, Iseki K.  The mechanism of carrier-mediated transport of folates in BeWo cells: the involvement of heme carrier protein 1 in placental folate transport  Biosci Biotechnol Biochem  2008  72(2):329-34  PubMed ID: 18256483   DOI: 10.1271/bbb.70347
8848  Yamamoto T, Kuniki K, Takekuma Y, Hirano T, Iseki K, Sugawara M.  Ribavirin uptake by cultured human choriocarcinoma (BeWo) cells and Xenopus laevis oocytes expressing recombinant plasma membrane human nucleoside transporters.  Eur. J. Pharmacol.  2007  557:1-8  PubMed ID: 17140564   DOI: 10.1016/j.ejphar.2006.10.062
2550  Tamura, H O, Taniguchi K, Hayashi E, Hiyoshi Y, Nagai F  Expression profiling of sulfotransferases in human cell lines derived from extra-hepatic tissues.  Biol Pharm Bull  2001  24:1258-62  PubMed ID: 11725959   DOI: 10.1248/bpb.24.1258

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