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細胞番号 : 細胞名
RCB1642 : 3T3 Swiss Albino  update : 2019/12/16
細胞特性(Comment:英)Contact inhibition. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 85022108 prior to order.
細胞特性(日)マウス線維芽細胞。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、ECA 85022108 を必ずご参照ください。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85022108 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、ECA85022108 を必ず参照すること。2) 商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 ECACC
寄託日 2000
動物種 _mouse < Mammals
動物系統名 Swiss Albino
embryo
採取組織 embryo/fetus, whole
細胞分類 other
入手歴 overseas, ECACC -> RIKEN Cell Bank
細胞寿命 infinite
細胞形態 fibroblast-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
播種細胞数 3 x 10^(5) cells/60 mm dish
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
動物種PCR OK
マウス系統検査 OK
アイソザイム検査 LD, NP
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 3件
Reference(日) 1件
利用者成果(英) 21件
利用者成果(日) 0件

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Reference(英)
4976  Todaro GJ, Habel K, Green H.  Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40.  Virology  1965  27(2):179-85  PubMed ID: 4284655
4977  TODARO GJ, GREEN H, GOLDBERG BD.  TRANSFORMATION OF PROPERTIES OF AN ESTABLISHED CELL LINE BY SV40 AND POLYOMA VIRUS.  Proc Natl Acad Sci U S A  1964  51:66-73  PubMed ID: 14104605
963  Todaro, G. and Green, H.  Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.  J Cell Biol  1963  17:299-313  PubMed ID: 13985244

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Reference(日)
1243  吉竹佳乃,西川克三  バイオテクノロジ-素材としての培養細胞 細胞周期  蛋白質 核酸 酵素  1988  33:1325-1326 

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利用者成果(英)
10406  D. Merzhakupova, S. Pascarelli, P. Laurino  Epidermal Growth Factor single mutants highlighted by homologs cross-conservation approach differentially affect the EGF Receptor downstream pathway  bioRxiv  2020  2020-7-17 
11916  Fukuta T, Nishikawa A, Kogure K.  Low level electricity increases the secretion of extracellular vesicles from cultured cells.  Biochem Biophys Rep  2020    PubMed ID: 31828227
12146  Shinya Yano, Takumi Iwase, Mitsuhiro Shibata, Yoshitaka Miyamoto, Toshiaki Shimasaki, Naozumi Teramoto  Synthesis of Photocrosslinkable Copolymers of Cinnamoyl Group-modified Methacrylate and 2-Hydroxyethyl Methacrylate, and Fibroblast Cell Growth on Their Thin Films  Journal of Photopolymer Science and Technology  2020   
12963  Saeki K, Hiramatsu H, Hori A, Hirai Y, Yamada M, Utoh R, Seki M.  Sacrificial Alginate-Assisted Microfluidic Engineering of Cell-Supportive Protein Microfibers for Hydrogel-Based Cell Encapsulation.  ACS Omega  2020    PubMed ID: 32905425
11387  Kodama Y, Harinath D, Mihara-Tomiyama N, Tominaga N, Ide Y, Nakahara T, Maeda M, Igarashi M, D'Armiento J, Chada K, Imai K.  Hmga2 regulation of tooth formation and association with Sox2 and Nanog expression.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30654938
5344  Tomoko Gowa Oyama, View ORCID ProfileKotaro Oyama, Atsushi Kimura, Fumiya Yoshida, Ryo Ishida, Masashi Yamazaki, Hiromi Miyoshi, View ORCID ProfileMitsumasa Taguchi  Elasticity and Topography-Controlled Collagen Hydrogels Mimicking Native Cellular Milieus  bioRxiv  2019  July 18 
10697  Yano S, Iwase T, Teramoto N, Shimasaki T, Shibata M.  Synthesis, thermal properties and cell-compatibility of photocrosslinked cinnamoyl-modified hydroxypropyl cellulose.  Carbohydr Polym  2018  184:418-426  PubMed ID: 29352937
10812  Morisaku T, Yui H.  Laser-induced surface deformation microscope for the study of the dynamic viscoelasticity of plasma membrane in a living cell.  Analyst  2018  143:2397-2404  PubMed ID: 29700531
11071  Tomoko Gowa Oyama, Bin Jeremiah Duenas Barba, Yuji Hosaka, and Mitsumasa Taguchi  Single-step fabrication of polydimethylsiloxane microwell arrays with long-lasting hydrophilic inner surfaces.  Appl. Phys. Lett.   2018  112, 213704 
4123  Morisaku T, Ishihara M, Yui H.  Discrimination between Normal and Cancerous Cells from Dynamic Viscoelastic Properties with a Laser-induced Surface Deformation Microscope.  Anal Sci  2018    PubMed ID: 29780041
10934    Cell migration guided by a groove with branches   Journal of Biomechanical Science and Engineering  2017  12 
7066  Inaba T, Kishimoto T, Murate M, Tajima T, Sakai S, Abe M, Makino A, Tomishige N, Ishitsuka R, Ikeda Y, Takeoka S, Kobayashi T.  Phospholipase Cβ1 induces membrane tubulation and is involved in caveolae formation.  Proc. Natl. Acad. Sci. U.S.A.  2016  113:7834-9  PubMed ID: 27342861
10855  Junk  Engineering thick cell sheets by electrochemical desorption of oligopeptides on membrane substrates  Regenerative Therapy  2016  3 : 24-31 
12945  Sugawara M, Miyoshi H, Miura T, Tanaka H, Tsubota KI, Liu H.  Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning  Biomed Res Int  2016  2016:5749749  PubMed ID: 28119928
14089  Enomoto J, Mochizuki N, Ebisawa K, Osaki T, Kageyama T, Myasnikova D, Nittami T, Fukuda J.  Engineering thick cell sheets by electrochemical desorption of oligopeptides on membrane substrates  Regen Ther  2016  3:24-31  PubMed ID: 31245469
7616  Mochizuki N, Kakegawa T, Osaki T, Sadr N, Kachouie NN, Suzuki H, Fukuda J.  Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide.  J Tissue Eng Regen Med  2011  7:236-43  PubMed ID: 22162306
7785  Fukuda J, Kameoka Y, Suzuki H.  Spatio-temporal detachment of single cells using microarrayed transparent electrodes.  Biomaterials  2011  32:6663-9  PubMed ID: 21665269
7892  Otani M, Taniguchi T, Sakai A, Seta J, Kadoyama K, Nakamura-Hirota T, Matsuyama S, Sano K, Takano M.  Phosphoproteome profiling using a fluorescent phosphosensor dye in two-dimensional polyacrylamide gel electrophoresis.  Appl. Biochem. Biotechnol.  2011  164:804-18  PubMed ID: 21384102
8211  Okuyama T, Yamazoe H, Mochizuki N, Khademhosseini A, Suzuki H, Fukuda J.  Preparation of arrays of cell spheroids and spheroid-monolayer cocultures within a microfluidic device.  J. Biosci. Bioeng.  2010  110:572-6  PubMed ID: 20591731
8234  Okuyama T, Yamazoe H, Seto Y, Suzuki H, Fukuda J.  Cell micropatterning inside a microchannel and assays under a stable concentration gradient.  J. Biosci. Bioeng.  2010  110:230-7  PubMed ID: 20547384
8607  Inaba R, Khademhosseini A, Suzuki H, Fukuda J.  Electrochemical desorption of self-assembled monolayers for engineering cellular tissues.  Biomaterials  2009  30:3573-9  PubMed ID: 19362363

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