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細胞番号 : 細胞名
RCB1642 : 3T3 Swiss Albino  update : 2023/05/25
細胞特性(Comment:英)Contact inhibition. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85022108 prior to order.
細胞特性(日)マウス線維芽細胞。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、ECACC 85022108 を必ずご参照ください。
使用条件(英)1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85022108 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、ECA85022108 を必ず参照すること。2) 商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 ECACC
寄託日 2000
動物種 mouse < Mammals
動物系統名 Swiss Albino
採取組織 embryo/fetus, whole
細胞分類 other
入手歴 overseas, ECACC -> RIKEN Cell Bank
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_0120
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
播種細胞数 3 x 10^(5) cells/60 mm dish
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
文献情報 Reference(英) 3件
Reference(日) 1件
利用者成果(英) 31件
利用者成果(日) 0件

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21067  Yano S, Yamaguchi K, Shibata M, Ifuku S, Teramoto N.  Photocrosslinked Fish Collagen Peptide/Chitin Nanofiber Composite Hydrogels from Marine Resources: Preparation, Mechanical Properties, and an In Vitro Study.  Polymers (Basel)  2023  15(3)  PubMed ID: 36771982   DOI: 10.3390/polym15030682
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15520  Tomoko Gowa Oyama, Kotaro Oyama, Hiromi Miyoshi, Mitsumasa Taguchi  3D cell sheets formed via cell-driven buckling-delamination of patterned thin films  Materials & Design  2021  208:109975    DOI: 10.1016/j.matdes.2021.109975
16271  Matsuura S, Takayama T, Oyama TG, Oyama K, Taguchi M, Endo T, Akai T, Isaji T, Hoshina K.  A Radiation-Crosslinked Gelatin Hydrogel That Promotes Tissue Incorporation of an Expanded Polytetrafluoroethylene Vascular Graft in Rats  Biomolecules  2021  11(8):1105  PubMed ID: 34439772   DOI: 10.3390/biom11081105
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11916  Fukuta T, Nishikawa A, Kogure K.  Low level electricity increases the secretion of extracellular vesicles from cultured cells.  Biochem Biophys Rep  2020    PubMed ID: 31828227   DOI: 10.1016/j.bbrep.2019.100713
12146  Shinya Yano, Takumi Iwase, Mitsuhiro Shibata, Yoshitaka Miyamoto, Toshiaki Shimasaki, Naozumi Teramoto  Synthesis of Photocrosslinkable Copolymers of Cinnamoyl Group-modified Methacrylate and 2-Hydroxyethyl Methacrylate, and Fibroblast Cell Growth on Their Thin Films  Journal of Photopolymer Science and Technology  2020      DOI: 10.2494/photopolymer.32.823
12963  Saeki K, Hiramatsu H, Hori A, Hirai Y, Yamada M, Utoh R, Seki M.  Sacrificial Alginate-Assisted Microfluidic Engineering of Cell-Supportive Protein Microfibers for Hydrogel-Based Cell Encapsulation.  ACS Omega  2020    PubMed ID: 32905425   DOI: 10.1021/acsomega.0c02385
11387  Kodama Y, Harinath D, Mihara-Tomiyama N, Tominaga N, Ide Y, Nakahara T, Maeda M, Igarashi M, D'Armiento J, Chada K, Imai K.  Hmga2 regulation of tooth formation and association with Sox2 and Nanog expression.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30654938   DOI: 10.1016/j.bbrc.2019.01.017
5344  Tomoko Gowa Oyama, View ORCID ProfileKotaro Oyama, Atsushi Kimura, Fumiya Yoshida, Ryo Ishida, Masashi Yamazaki, Hiromi Miyoshi, View ORCID ProfileMitsumasa Taguchi  Elasticity and Topography-Controlled Collagen Hydrogels Mimicking Native Cellular Milieus  bioRxiv  2019  July 18    DOI: 10.1101/706952
10697  Yano S, Iwase T, Teramoto N, Shimasaki T, Shibata M.  Synthesis, thermal properties and cell-compatibility of photocrosslinked cinnamoyl-modified hydroxypropyl cellulose.  Carbohydr Polym  2018  184:418-426  PubMed ID: 29352937   DOI: 10.1016/j.carbpol.2017.12.087
10812  Morisaku T, Yui H.  Laser-induced surface deformation microscope for the study of the dynamic viscoelasticity of plasma membrane in a living cell.  Analyst  2018  143:2397-2404  PubMed ID: 29700531   DOI: 10.1039/c7an01620d
11071  Tomoko Gowa Oyama, Bin Jeremiah Duenas Barba, Yuji Hosaka, and Mitsumasa Taguchi  Single-step fabrication of polydimethylsiloxane microwell arrays with long-lasting hydrophilic inner surfaces.  Appl. Phys. Lett.   2018  112, 213704    DOI: 10.1063/1.5025076
4123  Morisaku T, Ishihara M, Yui H.  Discrimination between Normal and Cancerous Cells from Dynamic Viscoelastic Properties with a Laser-induced Surface Deformation Microscope.  Anal Sci  2018    PubMed ID: 29780041   DOI: 10.2116/analsci.18P170
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14673  Gullo MR, Takeuchi S, Paul O.  Multicellular Biohybrid Materials: Probing the Interplay of Cells of Different Types Precisely Positioned and Constrained on 3D Wireframe-Like Microstructures  Adv Healthc Mater  2017  6(7)  PubMed ID: 28306220   DOI: 10.1002/adhm.201601053
7066  Inaba T, Kishimoto T, Murate M, Tajima T, Sakai S, Abe M, Makino A, Tomishige N, Ishitsuka R, Ikeda Y, Takeoka S, Kobayashi T.  Phospholipase Cβ1 induces membrane tubulation and is involved in caveolae formation.  Proc. Natl. Acad. Sci. U.S.A.  2016  113:7834-9  PubMed ID: 27342861   DOI: 10.1073/pnas.1603513113
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14089  Enomoto J, Mochizuki N, Ebisawa K, Osaki T, Kageyama T, Myasnikova D, Nittami T, Fukuda J.  Engineering thick cell sheets by electrochemical desorption of oligopeptides on membrane substrates  Regen Ther  2016  3:24-31  PubMed ID: 31245469   DOI: 10.1016/j.reth.2015.12.003
15219  Shinya Yano, Naozumi Teramoto, Rikako Miyamoto, Eiichi Nakajima, Kazuaki Hashimoto, Mitsuhiro Shibata  Fibroblast cell proliferation on photo-cured trehalose cinnamoyl ester thin films  Journal of Bioactive and Compatible Polymers  2015  30:87-98    DOI: 10.1177/0883911514558012
15457  Hiroyoshi Aoki, Hiromi Miyoshi, Yutaka Yamagata  Electrospinning of gelatin nanofiber scaffolds with mild neutral cosolvents for use in tissue engineering  Polymer Journal  2015  47:267-277    DOI: 10.1038/pj.2014.94
7616  Mochizuki N, Kakegawa T, Osaki T, Sadr N, Kachouie NN, Suzuki H, Fukuda J.  Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide.  J Tissue Eng Regen Med  2013  7:236-43  PubMed ID: 22162306   DOI: 10.1002/term.519
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8521  Yamazoe H, Okuyama T, Suzuki H, Fukuda J.  Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.  Acta Biomater  2010  6:526-33  PubMed ID: 19654059   DOI: 10.1016/j.actbio.2009.07.036
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