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Cell No. : Cell Name
RCB1642 : 3T3 Swiss Albino  update : 2023/07/11
CommentContact inhibition. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85022108 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85022108 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Year of deposit 2000
Animal _mouse < Mammals
Strain name Swiss Albino
Age at sampling embryo
Tissue embryo/fetus, whole
Classification other
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0120
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 3 x 10 5 cells/60 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 29

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4976  Todaro GJ, Habel K, Green H.  Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40.  Virology  1965  27(2):179-85  PubMed ID: 4284655   DOI: 10.1016/0042-6822(65)90157-1
4977  TODARO GJ, GREEN H, GOLDBERG BD.  TRANSFORMATION OF PROPERTIES OF AN ESTABLISHED CELL LINE BY SV40 AND POLYOMA VIRUS.  Proc Natl Acad Sci U S A  1964  51:66-73  PubMed ID: 14104605   DOI: 10.1073/pnas.51.1.66
963  Todaro, G. and Green, H.  Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.  J Cell Biol  1963  17:299-313  PubMed ID: 13985244   DOI: 10.1083/jcb.17.2.299

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User's Publication
21067  Yano S, Yamaguchi K, Shibata M, Ifuku S, Teramoto N.  Photocrosslinked Fish Collagen Peptide/Chitin Nanofiber Composite Hydrogels from Marine Resources: Preparation, Mechanical Properties, and an In Vitro Study.  Polymers (Basel)  2023  15(3)  PubMed ID: 36771982   DOI: 10.3390/polym15030682
16848  Ahmed E. Swilem, Tomoko Gowa Oyama, Kotaro Oyama, Atsushi Kimura, Mitsumasa Taguchi  Development of carboxymethyl cellulose/gelatin hybrid hydrogels via radiation-induced cross-linking as novel anti-adhesion barriers  Polymer Degradation and Stability  2022  197:109856    DOI: 10.1016/j.polymdegradstab.2022.109856
20156  Oyama TG, Oyama K, Kimura A, Yoshida F, Ishida R, Yamazaki M, Miyoshi H, Taguchi M.  Collagen hydrogels with controllable combined cues of elasticity and topography to regulate cellular processes.  Biomed Mater  2021    PubMed ID: 34030146   DOI: 10.1088/1748-605X/ac0452
14839  Pascarelli S, Merzhakupova D, Uechi GI, Laurino P.  Binding of single-mutant epidermal growth factor (EGF) ligands alters the stability of the EGF receptor dimer and promotes growth signaling  J Biol Chem  2021  297(1):100872  PubMed ID: 34126069   DOI: 10.1016/j.jbc.2021.100872
15520  Tomoko Gowa Oyama, Kotaro Oyama, Hiromi Miyoshi, Mitsumasa Taguchi  3D cell sheets formed via cell-driven buckling-delamination of patterned thin films  Materials & Design  2021  208:109975    DOI: 10.1016/j.matdes.2021.109975
16271  Matsuura S, Takayama T, Oyama TG, Oyama K, Taguchi M, Endo T, Akai T, Isaji T, Hoshina K.  A Radiation-Crosslinked Gelatin Hydrogel That Promotes Tissue Incorporation of an Expanded Polytetrafluoroethylene Vascular Graft in Rats  Biomolecules  2021  11(8):1105  PubMed ID: 34439772   DOI: 10.3390/biom11081105
11916  Fukuta T, Nishikawa A, Kogure K.  Low level electricity increases the secretion of extracellular vesicles from cultured cells.  Biochem Biophys Rep  2020    PubMed ID: 31828227   DOI: 10.1016/j.bbrep.2019.100713
12146  Shinya Yano, Takumi Iwase, Mitsuhiro Shibata, Yoshitaka Miyamoto, Toshiaki Shimasaki, Naozumi Teramoto  Synthesis of Photocrosslinkable Copolymers of Cinnamoyl Group-modified Methacrylate and 2-Hydroxyethyl Methacrylate, and Fibroblast Cell Growth on Their Thin Films  Journal of Photopolymer Science and Technology  2020      DOI: 10.2494/photopolymer.32.823
12963  Saeki K, Hiramatsu H, Hori A, Hirai Y, Yamada M, Utoh R, Seki M.  Sacrificial Alginate-Assisted Microfluidic Engineering of Cell-Supportive Protein Microfibers for Hydrogel-Based Cell Encapsulation.  ACS Omega  2020    PubMed ID: 32905425   DOI: 10.1021/acsomega.0c02385
11387  Kodama Y, Harinath D, Mihara-Tomiyama N, Tominaga N, Ide Y, Nakahara T, Maeda M, Igarashi M, D'Armiento J, Chada K, Imai K.  Hmga2 regulation of tooth formation and association with Sox2 and Nanog expression.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30654938   DOI: 10.1016/j.bbrc.2019.01.017
10697  Yano S, Iwase T, Teramoto N, Shimasaki T, Shibata M.  Synthesis, thermal properties and cell-compatibility of photocrosslinked cinnamoyl-modified hydroxypropyl cellulose.  Carbohydr Polym  2018  184:418-426  PubMed ID: 29352937   DOI: 10.1016/j.carbpol.2017.12.087
10812  Morisaku T, Yui H.  Laser-induced surface deformation microscope for the study of the dynamic viscoelasticity of plasma membrane in a living cell.  Analyst  2018  143:2397-2404  PubMed ID: 29700531   DOI: 10.1039/c7an01620d
11071  Tomoko Gowa Oyama, Bin Jeremiah Duenas Barba, Yuji Hosaka, and Mitsumasa Taguchi  Single-step fabrication of polydimethylsiloxane microwell arrays with long-lasting hydrophilic inner surfaces.  Appl. Phys. Lett.   2018  112, 213704    DOI: 10.1063/1.5025076
4123  Morisaku T, Ishihara M, Yui H.  Discrimination between Normal and Cancerous Cells from Dynamic Viscoelastic Properties with a Laser-induced Surface Deformation Microscope.  Anal Sci  2018    PubMed ID: 29780041   DOI: 10.2116/analsci.18P170
10934    Cell migration guided by a groove with branches   Journal of Biomechanical Science and Engineering  2017  12    DOI: 10.1299/jbse.16-00613
14673  Gullo MR, Takeuchi S, Paul O.  Multicellular Biohybrid Materials: Probing the Interplay of Cells of Different Types Precisely Positioned and Constrained on 3D Wireframe-Like Microstructures  Adv Healthc Mater  2017  6(7)  PubMed ID: 28306220   DOI: 10.1002/adhm.201601053
7066  Inaba T, Kishimoto T, Murate M, Tajima T, Sakai S, Abe M, Makino A, Tomishige N, Ishitsuka R, Ikeda Y, Takeoka S, Kobayashi T.  Phospholipase Cβ1 induces membrane tubulation and is involved in caveolae formation.  Proc. Natl. Acad. Sci. U.S.A.  2016  113:7834-9  PubMed ID: 27342861   DOI: 10.1073/pnas.1603513113
12945  Sugawara M, Miyoshi H, Miura T, Tanaka H, Tsubota KI, Liu H.  Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning  Biomed Res Int  2016  2016:5749749  PubMed ID: 28119928   DOI: 10.1155/2016/5749749
14089  Enomoto J, Mochizuki N, Ebisawa K, Osaki T, Kageyama T, Myasnikova D, Nittami T, Fukuda J.  Engineering thick cell sheets by electrochemical desorption of oligopeptides on membrane substrates  Regen Ther  2016  3:24-31  PubMed ID: 31245469   DOI: 10.1016/j.reth.2015.12.003
15219  Shinya Yano, Naozumi Teramoto, Rikako Miyamoto, Eiichi Nakajima, Kazuaki Hashimoto, Mitsuhiro Shibata  Fibroblast cell proliferation on photo-cured trehalose cinnamoyl ester thin films  Journal of Bioactive and Compatible Polymers  2015  30:87-98    DOI: 10.1177/0883911514558012
15457  Hiroyoshi Aoki, Hiromi Miyoshi, Yutaka Yamagata  Electrospinning of gelatin nanofiber scaffolds with mild neutral cosolvents for use in tissue engineering  Polymer Journal  2015  47:267-277    DOI: 10.1038/pj.2014.94
7616  Mochizuki N, Kakegawa T, Osaki T, Sadr N, Kachouie NN, Suzuki H, Fukuda J.  Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide.  J Tissue Eng Regen Med  2013  7:236-43  PubMed ID: 22162306   DOI: 10.1002/term.519
15749  Fukuda J, Takahashi S, Osaki T, Mochizuki N, Suzuki H.  Processing of nanolitre liquid plugs for microfluidic cell-based assays  Sci Technol Adv Mater  2012  13(6):064201  PubMed ID: 27877528   DOI: 10.1088/1468-6996/13/6/064201
7785  Fukuda J, Kameoka Y, Suzuki H.  Spatio-temporal detachment of single cells using microarrayed transparent electrodes.  Biomaterials  2011  32:6663-9  PubMed ID: 21665269   DOI: 10.1016/j.biomaterials.2011.05.068
7892  Otani M, Taniguchi T, Sakai A, Seta J, Kadoyama K, Nakamura-Hirota T, Matsuyama S, Sano K, Takano M.  Phosphoproteome profiling using a fluorescent phosphosensor dye in two-dimensional polyacrylamide gel electrophoresis.  Appl. Biochem. Biotechnol.  2011  164:804-18  PubMed ID: 21384102   DOI: 10.1007/s12010-011-9175-5
8211  Okuyama T, Yamazoe H, Mochizuki N, Khademhosseini A, Suzuki H, Fukuda J.  Preparation of arrays of cell spheroids and spheroid-monolayer cocultures within a microfluidic device.  J. Biosci. Bioeng.  2010  110:572-6  PubMed ID: 20591731   DOI: 10.1016/j.jbiosc.2010.05.013
8234  Okuyama T, Yamazoe H, Seto Y, Suzuki H, Fukuda J.  Cell micropatterning inside a microchannel and assays under a stable concentration gradient.  J. Biosci. Bioeng.  2010  110:230-7  PubMed ID: 20547384   DOI: 10.1016/j.jbiosc.2010.02.001
8521  Yamazoe H, Okuyama T, Suzuki H, Fukuda J.  Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.  Acta Biomater  2010  6:526-33  PubMed ID: 19654059   DOI: 10.1016/j.actbio.2009.07.036
8607  Inaba R, Khademhosseini A, Suzuki H, Fukuda J.  Electrochemical desorption of self-assembled monolayers for engineering cellular tissues.  Biomaterials  2009  30:3573-9  PubMed ID: 19362363   DOI: 10.1016/j.biomaterials.2009.03.045

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