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Cell No. : Cell Name
RCB1642 : 3T3 Swiss Albino  update : 2019/12/16
CommentContact inhibition. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 85022108 prior to order.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85022108 prior to order. In addition, regarding commercial use and use for patent filing the users of this cell line must contact RIKEN BRC prior to order.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor ECACC
Year of deposit 2000
Animal mouse < Mammals
Strain name Swiss Albino
Age at sampling embryo
Tissue embryo/fetus, whole
Classification other
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 19

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4976  Todaro GJ, Habel K, Green H.  Antigenic and cultural properties of cells doubly transformed by polyoma virus and SV40.  Virology  1965  27(2):179-85  PubMed ID: 4284655
963  Todaro, G. and Green, H.  Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.  J Cell Biol  1963  17:299-313  PubMed ID: 13985244

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User's Publication
10406  D. Merzhakupova, S. Pascarelli, P. Laurino  Epidermal Growth Factor single mutants highlighted by homologs cross-conservation approach differentially affect the EGF Receptor downstream pathway  bioRxiv  2020  2020-7-17 
11916  Fukuta T, Nishikawa A, Kogure K.  Low level electricity increases the secretion of extracellular vesicles from cultured cells.  Biochem Biophys Rep  2020    PubMed ID: 31828227
12146  Shinya Yano, Takumi Iwase, Mitsuhiro Shibata, Yoshitaka Miyamoto, Toshiaki Shimasaki, Naozumi Teramoto  Synthesis of Photocrosslinkable Copolymers of Cinnamoyl Group-modified Methacrylate and 2-Hydroxyethyl Methacrylate, and Fibroblast Cell Growth on Their Thin Films  Journal of Photopolymer Science and Technology  2020   
11387  Kodama Y, Harinath D, Mihara-Tomiyama N, Tominaga N, Ide Y, Nakahara T, Maeda M, Igarashi M, D'Armiento J, Chada K, Imai K.  Hmga2 regulation of tooth formation and association with Sox2 and Nanog expression.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30654938
5344  Tomoko Gowa Oyama, View ORCID ProfileKotaro Oyama, Atsushi Kimura, Fumiya Yoshida, Ryo Ishida, Masashi Yamazaki, Hiromi Miyoshi, View ORCID ProfileMitsumasa Taguchi  Elasticity and Topography-Controlled Collagen Hydrogels Mimicking Native Cellular Milieus  bioRxiv  2019  July 18 
10697  Yano S, Iwase T, Teramoto N, Shimasaki T, Shibata M.  Synthesis, thermal properties and cell-compatibility of photocrosslinked cinnamoyl-modified hydroxypropyl cellulose.  Carbohydr Polym  2018  184:418-426  PubMed ID: 29352937
10812  Morisaku T, Yui H.  Laser-induced surface deformation microscope for the study of the dynamic viscoelasticity of plasma membrane in a living cell.  Analyst  2018  143:2397-2404  PubMed ID: 29700531
11071  Tomoko Gowa Oyama, Bin Jeremiah Duenas Barba, Yuji Hosaka, and Mitsumasa Taguchi  Single-step fabrication of polydimethylsiloxane microwell arrays with long-lasting hydrophilic inner surfaces.  Appl. Phys. Lett.   2018  112, 213704 
4123  Morisaku T, Ishihara M, Yui H.  Discrimination between Normal and Cancerous Cells from Dynamic Viscoelastic Properties with a Laser-induced Surface Deformation Microscope.  Anal Sci  2018    PubMed ID: 29780041
10934    Cell migration guided by a groove with branches   Journal of Biomechanical Science and Engineering  2017  12 
4525  Michiko Sugawara  Change in cell shape during slow migration in Swiss 3T3 fibroblasts  The 5th Switzerland-Japan Workshop on Biomechanics SJB2017  2017   
7066  Inaba T, Kishimoto T, Murate M, Tajima T, Sakai S, Abe M, Makino A, Tomishige N, Ishitsuka R, Ikeda Y, Takeoka S, Kobayashi T.  Phospholipase Cβ1 induces membrane tubulation and is involved in caveolae formation.  Proc. Natl. Acad. Sci. U.S.A.  2016  113:7834-9  PubMed ID: 27342861
10855  Junk  Engineering thick cell sheets by electrochemical desorption of oligopeptides on membrane substrates  Regenerative Therapy  2016  3 : 24-31 
7616  Mochizuki N, Kakegawa T, Osaki T, Sadr N, Kachouie NN, Suzuki H, Fukuda J.  Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide.  J Tissue Eng Regen Med  2011  7:236-43  PubMed ID: 22162306
7785  Fukuda J, Kameoka Y, Suzuki H.  Spatio-temporal detachment of single cells using microarrayed transparent electrodes.  Biomaterials  2011  32:6663-9  PubMed ID: 21665269
7892  Otani M, Taniguchi T, Sakai A, Seta J, Kadoyama K, Nakamura-Hirota T, Matsuyama S, Sano K, Takano M.  Phosphoproteome profiling using a fluorescent phosphosensor dye in two-dimensional polyacrylamide gel electrophoresis.  Appl. Biochem. Biotechnol.  2011  164:804-18  PubMed ID: 21384102
8211  Okuyama T, Yamazoe H, Mochizuki N, Khademhosseini A, Suzuki H, Fukuda J.  Preparation of arrays of cell spheroids and spheroid-monolayer cocultures within a microfluidic device.  J. Biosci. Bioeng.  2010  110:572-6  PubMed ID: 20591731
8234  Okuyama T, Yamazoe H, Seto Y, Suzuki H, Fukuda J.  Cell micropatterning inside a microchannel and assays under a stable concentration gradient.  J. Biosci. Bioeng.  2010  110:230-7  PubMed ID: 20547384
8607  Inaba R, Khademhosseini A, Suzuki H, Fukuda J.  Electrochemical desorption of self-assembled monolayers for engineering cellular tissues.  Biomaterials  2009  30:3573-9  PubMed ID: 19362363

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