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Cell No. : Cell Name
RCB1641 : EL4  update : 2022/04/06
Comment9,10-dimethyl-1,2benzanthracene induced lymphoma. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 85023105 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85023105 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 2000
Animal mouse < Mammals
Tissue ascites
Disease name ascites lymphoma lymphoblast
Metastatic ability Yes
Classification cancer
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_0255
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, PepB
Images
deposit info
lot info
Reference information Reference 6
User's Publication 6


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Reference
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890
2468  Herberman RB  Serological analysis of cell surface antigens of tumors induced by murine leukemia virus.  J Natl Cancer Inst  1972  48:265-71  PubMed ID: 4119883
2471  Shevach, E M, Stobo, J D, Green I  Immunoglobulin and theta-bearing murine leukemias and lymphomas.  J Immunol  1972  108:1146-51  PubMed ID: 4112916
2470  Old, L J, Boyse, E A, Stockert E  The G (Gross) leukemia antigen.  Cancer Res  1965  25:813-9  PubMed ID: 4284252
2469    Studies in antibody response of mice to tumour inoculation.  Br J Cancer  1950  4:372  PubMed ID: 14801344

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User's Publication
20272  Ma Y, Yoshida T, Matoba K, Kida K, Shintani R, Piao Y, Jin J, Nishino T, Hanayama R.  Identification of small compounds regulating the secretion of extracellular vesicles via a TIM4-affinity ELISA.  Sci Rep  2021  11(1):13471  PubMed ID: 34188113
4184  Kimura Y, Negishi H, Matsuda A, Endo N, Hangai S, Inoue A, Nishio J, Taniguchi T, Yanai H.  Novel chemical compound SINCRO with dual function in STING-type I interferon and tumor cell death pathways.  Cancer Sci.  2018    PubMed ID: 29981256
14623  De Silva NH, Akazawa T, Wijewardana V, Inoue N, Oyamada M, Ohta A, Tachibana Y, Wijesekera DPH, Kuwamura M, Nishizawa Y, Itoh K, Izawa T, Hatoya S, Hasegawa T, Yamate J, Inaba T, Sugiura K.  Development of effective tumor immunotherapy using a novel dendritic cell-targeting Toll-like receptor ligand  PLoS One  2017  12(11):e0188738  PubMed ID: 29190690
14127  Kanda M, Yamanaka H, Kojo S, Usui Y, Honda H, Sotomaru Y, Harada M, Taniguchi M, Suzuki N, Atsumi T, Wada H, Baghdadi M, Seino K.  Transcriptional regulator Bhlhe40 works as a cofactor of T-bet in the regulation of IFN-γ production in iNKT cells  Proc Natl Acad Sci U S A  2016  113(24):E3394-402  PubMed ID: 27226296
6489  Ando T, Ichikawa J, Wako M, Hatsushika K, Watanabe Y, Sakuma M, Tasaka K, Ogawa H, Hamada Y, Yagita H, Nakao A.  TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells.  Arthritis Res Ther  2006  8(5):R146  PubMed ID: 16945157
6976  Nagai N, Anzawa T, Satoh Y, Suzuki T, Tajima K, Munekata M.  Activities of MC3T3-E1 cells cultured on gamma-irradiated salmon atelocollagen scaffold.  J Biosci Bioeng  2006  101(6):511-4  PubMed ID: 16935254



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