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Cell No. : Cell Name
RCB1641 : EL4  update : 2024/09/18
Comment9,10-dimethyl-1,2benzanthracene induced lymphoma. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85023105 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85023105 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 2000
Animal _mouse < Mammals
Tissue ascites
Disease name ascites lymphoma lymphoblast
Metastatic ability Yes
Classification cancer
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_0255
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, PepB
Images
deposit info
lot info
Reference information Reference 7
User's Publication 13


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Reference
922  Almeida JL, Dakic A, Kindig K, Kone M, Letham DLD, Langdon S, Peat R, Holding-Pillai J, Hall EM, Ladd M, Shaffer MD, Berg H, Li J, Wigger G, Lund S, Steffen CR, Fransway BB, Geraghty B, Natoli M, Bauer B, Gollin SM, Lewis DW, Reid Y.  Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines  PLoS One  2019  14(6):e0218412  PubMed ID: 31220119   DOI: 10.1371/journal.pone.0218412
716  Ralph P, Nakoinz I  Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS.  J Immunol  1977  119:950-54  PubMed ID: 894031  
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890   DOI: 10.1084/jem.143.6.1528
2468  Herberman RB  Serological analysis of cell surface antigens of tumors induced by murine leukemia virus.  J Natl Cancer Inst  1972  48:265-71  PubMed ID: 4119883  
2471  Shevach, E M, Stobo, J D, Green I  Immunoglobulin and theta-bearing murine leukemias and lymphomas.  J Immunol  1972  108:1146-51  PubMed ID: 4112916  
2470  Old, L J, Boyse, E A, Stockert E  The G (Gross) leukemia antigen.  Cancer Res  1965  25:813-9  PubMed ID: 4284252  
2469    Studies in antibody response of mice to tumour inoculation.  Br J Cancer  1950  4:372  PubMed ID: 14801344   DOI: 10.1038/bjc.1950.36

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User's Publication
21764  Kazaoka A, Fujimori S, Yamada Y, Shirayanagi T, Gao Y, Kuwahara S, Sakamoto N, Susukida T, Aoki S, Ito K.  HLA-B*57:01-dependent intracellular stress in keratinocytes triggers dermal hypersensitivity reactions to abacavir.  PNAS Nexus  2024  3(4):pgae140  PubMed ID: 38628599   DOI: 10.1093/pnasnexus/pgae140
18101  Sugimoto C, Murakami Y, Ishii E, Fujita H, Wakao H.  Reprogramming and redifferentiation of mucosal-associated invariant T cells reveal tumor inhibitory activity  Elife  2022  11:e70848  PubMed ID: 35379387   DOI: 10.7554/eLife.70848
16857  Naruse C, Sugihara K, Miyazaki T, Pan X, Sugiyama F, Asano M.  A degron system targeting endogenous PD-1 inhibits the growth of tumor cells in mice  NAR Cancer   2022  4(2):zcac019  PubMed ID: 35734392   DOI: 10.1093/narcan/zcac019
19499  Nguyen TD, Miyatake Y, Yoshida T, Kawahara H, Hanayama R.  Tumor-secreted proliferin-1 regulates adipogenesis and lipolysis in cachexia  Int J Cancer  2021  148(8):1982-1992  PubMed ID: 33252827   DOI: 10.1002/ijc.33418
20272  Ma Y, Yoshida T, Matoba K, Kida K, Shintani R, Piao Y, Jin J, Nishino T, Hanayama R.  Identification of small compounds regulating the secretion of extracellular vesicles via a TIM4-affinity ELISA.  Sci Rep  2021  11(1):13471  PubMed ID: 34188113   DOI: 10.1038/s41598-021-92860-2
4184  Kimura Y, Negishi H, Matsuda A, Endo N, Hangai S, Inoue A, Nishio J, Taniguchi T, Yanai H.  Novel chemical compound SINCRO with dual function in STING-type I interferon and tumor cell death pathways.  Cancer Sci.  2018    PubMed ID: 29981256   DOI: 10.1111/cas.13726
14623  De Silva NH, Akazawa T, Wijewardana V, Inoue N, Oyamada M, Ohta A, Tachibana Y, Wijesekera DPH, Kuwamura M, Nishizawa Y, Itoh K, Izawa T, Hatoya S, Hasegawa T, Yamate J, Inaba T, Sugiura K.  Development of effective tumor immunotherapy using a novel dendritic cell-targeting Toll-like receptor ligand  PLoS One  2017  12(11):e0188738  PubMed ID: 29190690   DOI: 10.1371/journal.pone.0188738
14127  Kanda M, Yamanaka H, Kojo S, Usui Y, Honda H, Sotomaru Y, Harada M, Taniguchi M, Suzuki N, Atsumi T, Wada H, Baghdadi M, Seino K.  Transcriptional regulator Bhlhe40 works as a cofactor of T-bet in the regulation of IFN-γ production in iNKT cells  Proc Natl Acad Sci U S A  2016  113(24):E3394-402  PubMed ID: 27226296   DOI: 10.1073/pnas.1604178113
18259  Acharya B, Terao S, Suzuki T, Naoe M, Hamada K, Mizuguchi H, Gotoh A.  Improving gene transfer in human renal carcinoma cells: Utilization of adenovirus vectors containing chimeric type 5 and type 35 fiber proteins  Exp Ther Med  2010  1(3):537-540  PubMed ID: 22993573   DOI: 10.3892/etm_00000085
19532  Kato K, Habu Y, Takaku H.  Non-sequence specific RNA inhibition of HIV-1 replication  Nucleic Acids Symp Ser (Oxf)  2007  (51):411-2  PubMed ID: 18029761   DOI: 10.1093/nass/nrm206
6489  Ando T, Ichikawa J, Wako M, Hatsushika K, Watanabe Y, Sakuma M, Tasaka K, Ogawa H, Hamada Y, Yagita H, Nakao A.  TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells.  Arthritis Res Ther  2006  8(5):R146  PubMed ID: 16945157   DOI: 10.1186/ar2038
6976  Nagai N, Anzawa T, Satoh Y, Suzuki T, Tajima K, Munekata M.  Activities of MC3T3-E1 cells cultured on gamma-irradiated salmon atelocollagen scaffold.  J Biosci Bioeng  2006  101(6):511-4  PubMed ID: 16935254   DOI: 10.1263/jbb.101.511
1264  Sakurai T, Wakimoto N, Yamada M, Shimamura S, Motoyoshi K.  Effect of macrophage colony-stimulating factor on mouse NK 1.1+ cell activity in vivo  Int J Immunopharmacol  1998  20(8):401-13  PubMed ID: 9778101   DOI: 10.1016/s0192-0561(98)00040-x


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