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Cell No. : Cell Name
RCB1639 : LoVo  update : 2022/04/05
CommentCEA-producing colon adenocarcinoma. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 87060101 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA87060101 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 56 years
Tissue colon, supraclavicular meta
Disease name colon adenocarcinoma
Metastastatic ability Yes
Metastatsd tissue left supraclavicular
Classification cancer
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0399
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 49-51(50) : 49(31),50(17),51(2)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 7
User's Publication 38


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Reference
2576  Melani C, Rivoltini L, Parmiani G, Calabretta B, Colombo, M P  Inhibition of proliferation by c-myb antisense oligodeoxynucleotides in colon adenocarcinoma cell lines that express c-myb.  Cancer Res  1991  51:2897-901  PubMed ID: 2032228  
2574  Matsubara N, Fuchimoto S, Orita K  Tumour necrosis factor-alpha induces translocation of protein kinase C in tumour necrosis factor-sensitive cell lines.  Immunology  1991  73:457-9  PubMed ID: 1916896  
2575  Grandi M, Geroni C, Giuliani, F C  Isolation and characterization of a human colon adenocarcinoma cell line resistant to doxorubicin.  Br J Cancer  1986  54:515-8  PubMed ID: 3756087   DOI: 10.1038/bjc.1986.206
4983  Drewinko B, Barlogie B, Freireich EJ.  Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives.  Cancer Res  1979  39(7 Pt 1):2630-6  PubMed ID: 445465  
2497  Drewinko B, Yang, L Y, Barlogie B, Romsdahl M, Meistrich M, Malahy, M A, Giovanella B  Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line.  J Natl Cancer Inst  1978  61:75-83  PubMed ID: 276641   DOI: 10.1093/jnci/61.1.75
2496  Drewinko B, Yand, L Y  Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells.  Exp Cell Res  1976  101:414-6  PubMed ID: 964319   DOI: 10.1016/0014-4827(76)90393-1
2495  Drewinko B, Romsdahl, M M, Yang, L Y, Ahearn, M J, Trujillo, J M  Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line.  Cancer Res  1976  36:467-75  PubMed ID: 1260746  

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User's Publication
16349  Takeda T, Yamamoto Y, Tsubaki M, Matsuda T, Kimura A, Shimo N, Nishida S.  PI3K/Akt/YAP signaling promotes migration and invasion of DLD-1 colorectal cancer cells  Oncol Lett  2022  23(4):106  PubMed ID: 35242234   DOI: 10.3892/ol.2022.13226
20660  Daitoku N, Miyamoto Y, Hiyoshi Y, Tokunaga R, Sakamoto Y, Sawayama H, Ishimoto T, Baba Y, Yoshida N, Baba H.  Activin A promotes cell proliferation, invasion and migration and predicts poor prognosis in patients with colorectal cancer.  Oncol Rep  2022  47(6)  PubMed ID: 35445735   DOI: 10.3892/or.2022.8318
20853  Uranbileg B, Kurano M, Kano K, Sakai E, Arita J, Hasegawa K, Nishikawa T, Ishihara S, Yamashita H, Seto Y, Ikeda H, Aoki J, Yatomi Y.  Sphingosine 1-phosphate lyase facilitates cancer progression through converting sphingolipids to glycerophospholipids.  Clin Transl Med  2022  12(9):e1056  PubMed ID: 36125914   DOI: 10.1002/ctm2.1056
6918  Nagasawa I, Muroi M, Kawatani M, Ohishi T, Ohba SI, Kawada M, Osada H.  Identification of a Small Compound Targeting PKM2-Regulated Signaling Using 2D Gel Electrophoresis-Based Proteome-wide CETSA.  Cell Chem Biol  2020  27(2):186-196  PubMed ID: 31813846   DOI: 10.1016/j.chembiol.2019.11.010
9913  Satoshi Inoue, Takumi Tsunoda, Miho Riku, Hideaki Ito, Akihito Inoko, Hideki Murakami, Masahide Ebi, Naotaka Ogasawara, Ira Pastan, Kunio Kasugai, Kenji Kasai, Hiroshi Ikeda, Shingo Inaguma  Diffuse Mesothelin Expression Leads to Worse Prognosis Through Enhanced Cellular Proliferation in Colorectal Cancer  Oncol Lett  2020  19(3):1741-1750  PubMed ID: 32194667   DOI: 10.3892/ol.2020.11290
11905  Igarashi H, Taniguchi H, Nosho K, Ishigami K, Koide H, Mitsuhashi K, Okita K, Takemasa I, Imai K, Nakase H.  PRDM14 promotes malignant phenotype and correlates with poor prognosis in colorectal cancer.  Clin Transl Oncol  2020  22(7):1126-1137  PubMed ID: 31741141   DOI: 10.1007/s12094-019-02239-z
13234  Nishimoto A, Takemoto Y, Saito T, Kurazumi H, Tanaka T, Harada E, Shirasawa B, Hamano K.  Nuclear β-catenin expression is positively regulated by JAB1 in human colorectal cancer cells.  Biochem Biophys Res Commun  2020    PubMed ID: 32977947   DOI: 10.1016/j.bbrc.2020.09.007
13502  Nambara S, Masuda T, Kobayashi Y, Sato K, Tobo T, Koike K, Noda M, Ogawa Y, Kuroda Y, Ito S, Eguchi H, Sugimachi K, Mimori K.  GTF2IRD1 on chromosome 7 is a novel oncogene regulating the tumor-suppressor gene TGFβR2 in colorectal cancer  Cancer Sci  2020  111(2):343-355  PubMed ID: 31758608   DOI: 10.1111/cas.14248
13590  Naka Y, Okada T, Nakagawa T, Kobayashi E, Kawasaki Y, Tanaka Y, Tawa H, Hirata Y, Kawakami K, Kakimoto K, Inoue T, Takeuchi T, Fukunishi S, Hirose Y, Uchiyama K, Asahi M, Higuchi K.  Enhancement of O-linked N-acetylglucosamine modification promotes metastasis in patients with colorectal cancer and concurrent type 2 diabetes mellitus  Oncol Lett  2020  20(2):1171-1178  PubMed ID: 32724357   DOI: 10.3892/ol.2020.11665
11913  Tsubaki M, Takeda T, Noguchi M, Jinushi M, Seki S, Morii Y, Shimomura K, Imano M, Satou T, Nishida S.  Overactivation of Akt Contributes to MEK Inhibitor Primary and Acquired Resistance in Colorectal Cancer Cells.  Cancers (Basel)  2019    PubMed ID: 31769426   DOI: 10.3390/cancers11121866
11009    Evidence suggesting sphingosine 1-phosphate may be actively generated, degraded and transported to extracellular spaces with increased S1P2 and S1P3 expressions in colon cancer  Clinical Colorectal Cancer  2018  17, e171-e182  PubMed ID: 29223361   DOI: 10.1016/j.clcc.2017.11.004
10185  Yokoi K, Yamashita K, Ishii S, Tanaka T, Nishizawa N, Tsutsui A, Miura H, Katoh H, Yamanashi T, Naito M, Sato T, Nakamura T, Watanabe M.  Comprehensive molecular exploration identified promoter DNA methylation of the CRBP1 gene as a determinant of radiation sensitivity in rectal cancer.  Br. J. Cancer  2017  116:1046-1056  PubMed ID: 28291773   DOI: 10.1038/bjc.2017.65
10296  Gombodorj N, Yokobori T, Yoshiyama S, Kawabata-Iwakawa R, Rokudai S, Horikoshi I, Nishiyama M, Nakano T.  Inhibition of Ubiquitin-conjugating Enzyme E2 May Activate the Degradation of Hypoxia-inducible Factors and, thus, Overcome Cellular Resistance to Radiation in Colorectal Cancer.  Anticancer Res.  2017  37:2425-2436  PubMed ID: 28476810   DOI: 10.21873/anticanres.11582
10498  Inaguma S, Lasota J, Felisiak-Golabek A, Kowalik A, Wang Z, Zieba S, Kalisz J, Ikeda H, Miettinen M.  Histopathological and genotypic characterization of metastatic colorectal carcinoma with PD-L1 (CD274)-expression: Possible roles of tumour micro environmental factors for CD274 expression.  J Pathol Clin Res  2017  3:268-278  PubMed ID: 29085667   DOI: 10.1002/cjp2.81
14055  Morishita S, Suzuki T, Niwa Y, Dohmae N, Simizu S.  Dpy-19 like 3-mediated C-mannosylation and expression levels of RPE-spondin in human tumor cell lines  Oncol Lett  2017  14(2):2537-2544  PubMed ID: 28781692   DOI: 10.3892/ol.2017.6465
14618  Ishimura E, Nakagawa T, Moriwaki K, Hirano S, Matsumori Y, Asahi M.  Augmented O-GlcNAcylation of AMP-activated kinase promotes the proliferation of LoVo cells, a colon cancer cell line  Cancer Sci  2017  108(12):2373-2382  PubMed ID: 28973823   DOI: 10.1111/cas.13412
9551  Kasai T, Nakanishi T, Ohno Y, Shimada H, Nakamura Y, Arakawa H, Tamai I.  Role of OATP2A1 in PGE(2) secretion from human colorectal cancer cells via exocytosis in response to oxidative stress.  Exp. Cell Res.  2016  341:123-31  PubMed ID: 26850138   DOI: 10.1016/j.yexcr.2016.02.002
9637  Li W, Itou J, Tanaka S, Nishimura T, Sato F, Toi M.  A homeobox protein, NKX6.1, up-regulates interleukin-6 expression for cell growth in basal-like breast cancer cells.  Exp. Cell Res.  2016  343:177-189  PubMed ID: 27032575   DOI: 10.1016/j.yexcr.2016.03.023
12615  Tokunaga R, Sakamoto Y, Nakagawa S, Miyake K, Izumi D, Kosumi K, Taki K, Higashi T, Imamura Y, Ishimoto T, Iwatsuki M, Baba Y, Miyamoto Y, Yoshida N, Oki E, Watanabe M, Baba H.  The Prognostic Significance of Histone Lysine Demethylase JMJD3/KDM6B in Colorectal Cancer  Ann Surg Oncol  2016  23(2):678-85  PubMed ID: 26416711   DOI: 10.1245/s10434-015-4879-3
14105  Matsuda Y, Miura K, Yamane J, Shima H, Fujibuchi W, Ishida K, Fujishima F, Ohnuma S, Sasaki H, Nagao M, Tanaka N, Satoh K, Naitoh T, Unno M.  SERPINI1 regulates epithelial-mesenchymal transition in an orthotopic implantation model of colorectal cancer  Cancer Sci  2016  107(5):619-28  PubMed ID: 26892864   DOI: 10.1111/cas.12909
10158  Takuma Yoshinaga, Takamasa Shigemitsu, Hiroto Nishimata, Masaki Kitazono, Emiko Hori, Ayako Tomiyoshi, Takayuki Takei, Masahiro Yoshida  Angiopoietin-like Protein 2 as a Potential Biomarker for Colorectal Cancer  Mol Clin Oncol  2015  3(5):1080-1084  PubMed ID: 26623054   DOI: 10.3892/mco.2015.577
14459  Ogawa H, Wu X, Kawamoto K, Nishida N, Konno M, Koseki J, Matsui H, Noguchi K, Gotoh N, Yamamoto T, Miyata K, Nishiyama N, Nagano H, Yamamoto H, Obika S, Kataoka K, Doki Y, Mori M, Ishii H.  MicroRNAs Induce Epigenetic Reprogramming and Suppress Malignant Phenotypes of Human Colon Cancer Cells  PLoS One  2015  10(5):e0127119  PubMed ID: 25970424   DOI: 10.1371/journal.pone.0127119
5452  Abe N, Hou DX, Munemasa S, Murata Y, Nakamura Y.  Nuclear factor-kappaB sensitizes to benzyl isothiocyanate-induced antiproliferation in p53-deficient colorectal cancer cells.  Cell Death Dis  2014  5:e1534  PubMed ID: 25412312   DOI: 10.1038/cddis.2014.495
12700  Mashima H, Ohno H, Yamada Y, Sakai T, Ohnishi H.  INSL5 may be a unique marker of colorectal endocrine cells and neuroendocrine tumors  Biochem Biophys Res Commun  2013  432(4):586-92  PubMed ID: 23438439   DOI: 10.1016/j.bbrc.2013.02.042
12714  Kawamura M, Toiyama Y, Tanaka K, Saigusa S, Okugawa Y, Hiro J, Uchida K, Mohri Y, Inoue Y, Kusunoki M.  CXCL5, a promoter of cell proliferation, migration and invasion, is a novel serum prognostic marker in patients with colorectal cancer  Eur J Cancer  2012  48(14):2244-51.  PubMed ID: 22197219   DOI: 10.1016/j.ejca.2011.11.032
12724  Matsushita K, Toiyama Y, Tanaka K, Saigusa S, Hiro J, Uchida K, Inoue Y, Kusunoki M.  Soluble CXCL16 in preoperative serum is a novel prognostic marker and predicts recurrence of liver metastases in colorectal cancer patients  Ann Surg Oncol  2012  19 Suppl 3:S518-27  PubMed ID: 21845497   DOI: 10.1245/s10434-011-1993-8
5870  Takahashi N, Fukushima T, Yorita K, Tanaka H, Chijiiwa K, Kataoka H.  Dickkopf-1 is overexpressed in human pancreatic ductal adenocarcinoma cells and is involved in invasive growth.  Int J Cancer.  2010  126(7):1611-20  PubMed ID: 19711349   DOI: 10.1002/ijc.24865
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
8700  Ishii S, Katsumura T, Shiozuka C, Ooyauchi K, Kawasaki K, Takigawa S, Fukushima T, Tokuji Y, Kinoshita M, Ohnishi M, Kawahara M, Ohba K.  Anti-inflammatory effect of buckwheat sprouts in lipopolysaccharide-activated human colon cancer cells and mice.  Biosci. Biotechnol. Biochem.  2008  72:3148-57  PubMed ID: 19060399   DOI: 10.1271/bbb.80324
15636  Sasaki H, Miura K, Horii A, Kaneko N, Fujibuchi W, Kiseleva L, Gu Z, Murata Y, Karasawa H, Mizoi T, Kobayashi T, Kinouchi M, Ohnuma S, Yazaki N, Unno M, Sasaki I.  Orthotopic implantation mouse model and cDNA microarray analysis indicates several genes potentially involved in lymph node metastasis of colorectal cancer  Cancer Sci  2008  99(4):711-9  PubMed ID: 18307535   DOI: 10.1111/j.1349-7006.2008.00725.x
3771  Tsushimi T, Noshima S, Oga A, Esato K, Sasaki K.  DNA amplification and chromosomal translocations are accompanied by chromosomal instability: analysis of seven human colon cancer cell lines by comparative genomic hybridization and spectral karyotyping.  Cancer Genet Cytogenet  2001  126(1):34-8  PubMed ID: 11343776   DOI: 10.1016/s0165-4608(00)00391-5
3772  Ijichi H, Ikenoue T, Kato N, Mitsuno Y, Togo G, Kato J, Kanai F, Shiratori Y, Omata M.  Systematic analysis of the TGF-beta-Smad signaling pathway in gastrointestinal cancer cells.  Biochem Biophys Res Commun  2001  289(2):350-7  PubMed ID: 11716479   DOI: 10.1006/bbrc.2001.5988
3764  Uchino H, Kataoka H, Itoh H, Hamasuna R, Koono M.  Overexpression of intestinal trefoil factor in human colon carcinoma cells reduces cellular growth in vitro and in vivo.  Gastroenterology  2000  118(1):60-9  PubMed ID: 10611154   DOI: 10.1016/s0016-5085(00)70414-8
17263  Seguchi K, Kataoka H, Uchino H, Nabeshima K, Koono M.  Secretion of protease nexin-II/amyloid beta protein precursor by human colorectal carcinoma cells and its modulation by cytokines/growth factors and proteinase inhibitors  Biol Chem  1999  80(4):473-83  PubMed ID: 10355633   DOI: 10.1515/BC.1999.061
3754  Nabeshima K, Shimao Y, Inoue T, Itoh H, Kataoka H, Koono M.  Hepatocyte growth factor/scatter factor induces not only scattering but also cohort migration of human colorectal-adenocarcinoma cells.  Int J Cancer  1998  78(6):750-9  PubMed ID: 9833769   DOI: 10.1002/(sici)1097-0215(19981209)78:6<750::aid-ijc13>3.0.co;2-#
3752  Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A, Koono M.  Analysis of tissue factor and tissue factor pathway inhibitor expression in human colorectal carcinoma cell lines and metastatic sublines to the liver.  Int J Cancer  1997  72(5):878-84  PubMed ID: 9311608   DOI: 10.1002/(sici)1097-0215(19970904)72:5<878::aid-ijc27>3.0.co;2-3
17245  Kataoka H, Meng JY, Uchino H, Nabeshima K, Kihira Y, Matuo Y, Koono M.  Modulation of matrix metalloproteinase-7 (matrilysin) secretion in coculture of human colon carcinoma cells with fibroblasts from orthotopic and ectopic organs  Oncol Res  1997  9(3):101-9  PubMed ID: 9220495  
12856  Tsuji S, Kawano S, Sawaoka H, Takei Y, Kobayashi I, Nagano K, Fusamoto H, Kamada T.  Evidences for involvement of cyclooxygenase-2 in proliferation of two gastrointestinal cancer cell lines  Prostaglandins Leukot Essent Fatty Acids  1996  ;55(3):179-83  PubMed ID: 8931116   DOI: 10.1016/s0952-3278(96)90095-2



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