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細胞番号 : 細胞名
RCB1638 : Hepa 1-6  update : 2021/04/15
細胞特性(Comment:英)Mouse cell line derived from hepatoma. Derived from BW7756 tumour in a C57L mouse. Secretes several products such as albumin, alpha-fetoprotein, alpha 1-antitrypin and amylase. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 92110305 prior to order.
細胞特性(日)マウス肝細胞癌由来細胞株。albumin, alpha-fetoprotein, alpha 1-antitrypin, amylaseなどを分泌。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、ECA 92110305 を必ずご参照ください。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA92110305 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、ECA92110305 を必ず参照すること。2) 商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 ECACC
寄託日 2000
動物種 _mouse < Mammals
動物系統名 C57L
採取組織 liver
病名 hepatopma
細胞分類 cancer
入手歴 overseas, ECACC -> RIKEN Cell Bank
細胞寿命 infinite
細胞形態 epithelial-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (high glucose) + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 1-2 times/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 42件
利用者成果(日) 0件

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Reference(英)
2679  Darlington, G J  Liver cell lines.  Methods Enzymol  1987  151:19-38  PubMed ID: 3431441
2494  Darlington, G J, Bernhard, H P, Miller, R A, Ruddle, F H  Expression of liver phenotypes in cultured mouse hepatoma cells.  J Natl Cancer Inst  1980  64:809-19  PubMed ID: 6102619

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Reference(日)

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利用者成果(英)
13346  Kobayashi S, Harada Y, Homma T, Yokoyama C, Fujii J.  Characterization of a rat monoclonal antibody raised against ferroptotic cells.  J Immunol Methods  2021    PubMed ID: 33189725
20021  Homma T, Kobayashi S, Sato H, Fujii J.  Superoxide produced by mitochondrial complex III plays a pivotal role in the execution of ferroptosis induced by cysteine starvation.  Arch Biochem Biophys  2021  700:108775  PubMed ID: 33493440
20131  Siswanto FM, Oguro A, Imaoka S.  Sp1 is a substrate of Keap1 and regulates the activity of CRL4AWDR23 ubiquitin ligase toward Nrf2.  J Biol Chem  2021  100704  PubMed ID: 33895141
11951  Liew LC, Gailhouste L, Tan GC, Yamamoto Y, Takeshita F, Nakagama H, Ochiya T.  MicroRNA-124a inhibits endoderm lineage commitment by targeting Sox17 and Gata6 in mouse embryonic stem cells.  Stem Cells  2020    PubMed ID: 31828873
12082  Kobayashi S, Ikeda Y, Shigeno Y, Konno H, Fujii J.  γ-Glutamylcysteine synthetase and γ-glutamyl transferase as differential enzymatic sources of γ-glutamylpeptides in mice.  Amino Acids  2020    PubMed ID: 32170467
12116  Noguchi T, Ikeda M, Murakami M, Masuzawa M, Imamura T, Hashimoto O, Matsui T, Funaba M.  Regulatory expression of bone morphogenetic protein 6 by 2,2'-dipyridyl.  Biochim Biophys Acta Gen Subj  2020    PubMed ID: 32251709
13060  Mayama S, Hamazaki N, Maruyama Y, Matsubara S, Kimura AP.  Transcriptional activation of the mouse Scd2 gene by interdependent enhancers and long noncoding RNAs in ovarian granulosa cells.  J Reprod Dev  2020    PubMed ID: 32507774
13073  Teratani T, Tomita K, Toma-Fukai S, Nakamura Y, Itoh T, Shimizu H, Shiraishi Y, Sugihara N, Higashiyama M, Shimizu T, Inoue I, Takenaka Y, Hokari R, Adachi T, Shimizu T, Miura S, Kanai T.  Redox-dependent PPARγ/Tnpo1 complex formation enhances PPARγ nuclear localization and signaling.  Free Radic Biol Med  2020    PubMed ID: 32553752
13090  Homma T, Kobayashi S, Fujii J.  Cysteine preservation confers resistance to glutathione-depleted cells against ferroptosis via CDGSH iron sulphur domain-containing proteins (CISDs).  Free Radic Res  2020    PubMed ID: 32605459
13566  Kim DH, Sadakane H, Nishikiori Y, Matsumura M, Ikeda M, Diao Z, Jha R, Murakami M, Matsui T, Funaba M.  Factors affecting expression and transcription of uncoupling protein 2 gene  J Vet Med Sci  2020  82(12):1734-1741  PubMed ID: 33162463
11550  Shigeto H, Ono T, Ikeda T, Hirota R, Ishida T, Kuroda A, Funabashi H.  Insulin sensor cells for the analysis of insulin secretion responses in single living pancreatic β cells.  Analyst  2019    PubMed ID: 31089611
11603  Aoki N, Matsui Y.  Comprehensive Analysis of Mouse Cancer/Testis Antigen Functions in Cancer Cells and Roles of TEKT5 in Cancer Cells and Testicular Germ Cells.  Mol. Cell. Biol.  2019    PubMed ID: 31208979
11769  Homma T, Kobayashi S, Fujii J.  Induction of ferroptosis by singlet oxygen generated from naphthalene endoperoxide.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31445701
11782  Homma T, Kobayashi S, Sato H, Fujii J.  Edaravone, a free radical scavenger, protects against ferroptotic cell death in vitro.  Exp. Cell Res.  2019    PubMed ID: 31479686
11821  Saparbay J, Tanaka Y, Tanimine N, Ohira M, Ohdan H.  Everolimus enhances TRAIL-mediated anti-tumor activity of liver resident natural killer cells in mice.  Transpl. Int.  2019    PubMed ID: 31560810
6951  Mizokami A, Mukai S, Gao J, Kawakubo-Yasukochi T4, Otani T5, Takeuchi H6, Jimi E7, Hirata M8.  GLP-1 signaling is required for improvement of glucose tolerance by osteocalcin.  J Endocrinol  2019    PubMed ID: 31693486
9192  Toru Suzuki, Chisato Kikuguchi, Saori Nishijima, Takeshi Nagashima, Akinori Takahashi, Mariko Okada, Tadashi Yamamoto  Postnatal Liver Functional Maturation Requires Cnot Complex-Mediated Decay of mRNAs Encoding Cell Cycle and Immature Liver Genes  Development  2019  146 (4)  PubMed ID: 30733279
10525  Mizunoe Y, Kobayashi M, Sudo Y, Watanabe S, Yasukawa H, Natori D, Hoshino A, Negishi A, Okita N, Komatsu M, Higami Y.  Trehalose protects against oxidative stress by regulating the Keap1-Nrf2 and autophagy pathways.  Redox Biol  2018  15:115-124  PubMed ID: 29241092
10795  Matsushita J, Okamura K, Nakabayashi K, Suzuki T, Horibe Y, Kawai T, Sakurai T, Yamashita S, Higami Y, Ichihara G, Hata K, Nohara K.  The DNA methylation profile of liver tumors in C3H mice and identification of differentially methylated regions involved in the regulation of tumorigenic genes.  BMC Cancer  2018  18:317  PubMed ID: 29566670
11103  Tsurudome Y, Koyanagi S, Kanemitsu T, Katamune C, Oda M, Kanado Y, Kato M, Morita A, Tahara Y, Matsunaga N, Shibata S, Ohdo S.  Circadian clock component PERIOD2 regulates diurnal expression of Na+/H+ exchanger regulatory factor-1 and its scaffolding function.  Sci Rep  2018  8(1):9072  PubMed ID: 29899468
11100  Nakano R, Ohira M, Yano T, Imaoka Y, Tanaka Y, Ohdan H.  Hepatic irradiation persistently eliminates liver resident NK cells.  PLoS ONE  2018  13(6):e0198904  PubMed ID: 29897952
11157  Germond A, Ichimura T, Chiu LD, Fujita K, Watanabe TM, Fujita H.  Cell type discrimination based on image features of molecular component distribution.  Sci Rep  2018  8(1):11726  PubMed ID: 30082723
4217  Shinto S, Matsuoka Y, Yamato M, Yamada KI.  Antioxidant nitroxides protect hepatic cells from oxidative stress-induced cell death.  J Clin Biochem Nutr  2018    PubMed ID: 29610552
10033  Nishihara M, Kanda GN, Suzuki T, Yamakado S, Harashima H, Kamiya H.  Enhanced transgene expression by plasmid-specific recruitment of histone acetyltransferase.  J. Biosci. Bioeng.  2017  123:277-280  PubMed ID: 27746157
10211  Fujita K, Iguchi Y, Une M, Watanabe S.  Ursodeoxycholic Acid Suppresses Lipogenesis in Mouse Liver: Possible Role of the Decrease in β-Muricholic Acid, a Farnesoid X Receptor Antagonist.  Lipids  2017  52:335-344  PubMed ID: 28315136
10364  Lee J, Homma T, Fujii J.  Mice in the early stage of liver steatosis caused by a high fat diet are resistant to thioacetamide-induced hepatotoxicity and oxidative stress.  Toxicol. Lett.  2017  277:92-103  PubMed ID: 28642009
10583  Lee J, Kang ES, Kobayashi S, Homma T, Sato H, Seo HG, Fujii J.  The viability of primary hepatocytes is maintained under a low cysteine-glutathione redox state with a marked elevation in ophthalmic acid production.  Exp. Cell Res.  2017  361:178-191  PubMed ID: 29079265
7067  Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M.  Fluorescence probes to detect lipid-derived radicals.  Nat. Chem. Biol.  2016  12:608-13  PubMed ID: 27294322
9733  Takahashi T, Sugawara W, Takiguchi Y, Takizawa K, Nakabayashi A, Nakamura M, Nagano-Ito M, Ichikawa S.  Identification of Plants That Inhibit Lipid Droplet Formation in Liver Cells: Rubus suavissimus Leaf Extract Protects Mice from High-Fat Diet-Induced Fatty Liver by Directly Affecting Liver Cells.  Evid Based Complement Alternat Med  2016  2016:4282758  PubMed ID: 27429636
12291  Saneyuki Ujino, Hironori Nishitsuji, Takayuki Hishiki, Kazuo Sugiyama, Hiroshi Takaku, Kunitada Shimotohno  Hepatitis C virus utilizes VLDLR as a novel entry pathway  Proc Natl Acad Sci U S A  2016  113(1):188-93  PubMed ID: 26699506
7330  Uyama T, Kawai K, Kono N, Watanabe M, Tsuboi K, Inoue T, Araki N, Arai H, Ueda N.  Interaction of Phospholipase A/Acyltransferase-3 with Pex19p: A POSSIBLE INVOLVEMENT IN THE DOWN-REGULATION OF PEROXISOMES.  J. Biol. Chem.  2015  290:17520-34  PubMed ID: 26018079
7395  Mutai H, Miya F, Fujii M, Tsunoda T, Matsunaga T.  Attenuation of progressive hearing loss in DBA/2J mice by reagents that affect epigenetic modifications is associated with up-regulation of the zinc importer Zip4.  PLoS ONE  2015  10:e0124301  PubMed ID: 25875282
13154  Hideaki Yamamoto, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira  Enhanced liver functions in mouse hepatoma cells by induced overexpression of liver-enriched transcription factor  Biochemical Engineering Journal  2012  60:67-73  
8206  Matsubara S, Takahashi T, Kimura AP.  Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression.  Gene  2010  465:17-29  PubMed ID: 20600704
8276  Miyayama T, Hiraoka D, Kawaji F, Nakamura E, Suzuki N, Ogra Y.  Roles of COMM-domain-containing 1 in stability and recruitment of the copper-transporting ATPase in a mouse hepatoma cell line.  Biochem. J.  2010  429:53-61  PubMed ID: 20433422
3356  Kubota, Koichi  A novel functional T cell hybridoma recognizes macrophage cell death induced by bacteria: a possible role for innate lymphocytes in bacterial infection.  J Immunol  2006  176:7576-88  PubMed ID: 16751404
3336  Kuang M, Liu, S Q, Saijo K, Uchimura E, Huang L, Leong, K W, Lu, M D, Huang, J F, Ohno T  Microwave tumour coagulation plus in situ treatment with cytokine-microparticles: induction of potent anti-residual tumour immunity.  Int J Hyperthermia  2005  21:247-57  PubMed ID: 16019851
3189  Ogawa S, Tagawa Y, Kamiyoshi A, Suzuki A, Nakayama J, Hashikura Y, Miyagawa S.  Crucial roles of mesodermal cell lineages in a murine embryonic stem cell-derived in vitro liver organogenesis system.  Stem Cells  2005  23:903-13  PubMed ID: 16043458
9007  Emoto M, Miki M, Sarker AH, Nakamura T, Seki Y, Seki S, Ikeda S.  Structure and transcription promoter activity of mouse flap endonuclease 1 gene: alternative splicing and bidirectional promoter.  Gene  2005  357:47-54  PubMed ID: 15967598
2705  Kushida S, Peng, B G, Uchimura E, Kuang M, Huang L, Miwa M, Ohno T  A tumour vaccine of fixed tumour fragments in a controlled-release vehicle with cytokines for therapy of hepatoma in mice.  Dig Liver Dis  2004  36:478-85  PubMed ID: 15285528
2637  Kasahara M, Watanabe Y, Sumasu M, Nagata T  A family of MHC class I-like genes located in the vicinity of the mouse leukocyte receptor complex.  Proc Natl Acad Sci U S A  2002  99:13687-92  PubMed ID: 12370446
2519  Zhang BN, Watanabe S, Kohyama M, Saijo K, Kusakabe M, Ohno T.  Tumor formation suppressed in gammadeltaT knock-out mice.  Cancer Lett  2000  153(1-2):63-6  PubMed ID: 10779631

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