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Cell No. : Cell Name
RCB1638 : Hepa 1-6  update : 2022/07/28
CommentMouse cell line derived from hepatoma. Derived from BW7756 tumour in a C57L mouse. Secretes several products such as albumin, alpha-fetoprotein, alpha 1-antitrypin and amylase. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 92110305 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA92110305 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Year of deposit 2000
Animal mouse < Mammals
Strain name C57L
Tissue liver
Disease name hepatopma
Classification cancer
History overseas, ECACC -> RIKEN Cell Bank
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0327
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 53

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2679  Darlington, G J  Liver cell lines.  Methods Enzymol  1987  151:19-38  PubMed ID: 3431441
2494  Darlington, G J, Bernhard, H P, Miller, R A, Ruddle, F H  Expression of liver phenotypes in cultured mouse hepatoma cells.  J Natl Cancer Inst  1980  64:809-19  PubMed ID: 6102619

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User's Publication
20637  Tagawa R, Kobayashi M, Sakurai M, Yoshida M, Kaneko H, Mizunoe Y, Nozaki Y, Okita N, Sudo Y, Higami Y.  Long-Term Dietary Taurine Lowers Plasma Levels of Cholesterol and Bile Acids.  Int J Mol Sci  2022  23(3)  PubMed ID: 35163722
20738  Yamamoto M, Miyata S.  Influences of Microscopic Imaging Conditions on Accuracy of Cell Morphology Discrimination Using Convolutional Neural Network of Deep Learning.  Micromachines (Basel)  2022  13(5)  PubMed ID: 35630227
13346  Kobayashi S, Harada Y, Homma T, Yokoyama C, Fujii J.  Characterization of a rat monoclonal antibody raised against ferroptotic cells.  J Immunol Methods  2021    PubMed ID: 33189725
20021  Homma T, Kobayashi S, Sato H, Fujii J.  Superoxide produced by mitochondrial complex III plays a pivotal role in the execution of ferroptosis induced by cysteine starvation.  Arch Biochem Biophys  2021  700:108775  PubMed ID: 33493440
20131  Siswanto FM, Oguro A, Imaoka S.  Sp1 is a substrate of Keap1 and regulates the activity of CRL4AWDR23 ubiquitin ligase toward Nrf2.  J Biol Chem  2021  100704  PubMed ID: 33895141
20245  Homma T, Kobayashi S, Conrad M, Konno H, Yokoyama C, Fujii J.  Nitric oxide protects against ferroptosis by aborting the lipid peroxidation chain reaction.  Nitric Oxide  2021  115:34-43  PubMed ID: 34329739
20279  Une N, Takano-Kasuya M, Kitamura N, Ohta M, Inose T, Kato C, Nishimura R, Tada H, Miyagi S, Ishida T, Unno M, Kamei T, Gonda K.  The anti-angiogenic agent lenvatinib induces tumor vessel normalization and enhances radiosensitivity in hepatocellular tumors.  Med Oncol  2021  38(6):60  PubMed ID: 33881631
11821  Saparbay J, Tanaka Y, Tanimine N, Ohira M, Ohdan H.  Everolimus enhances TRAIL-mediated anti-tumor activity of liver resident natural killer cells in mice.  Transpl. Int.  2020  33(2):229-243  PubMed ID: 31560810
6951  Mizokami A, Mukai S, Gao J, Kawakubo-Yasukochi T4, Otani T5, Takeuchi H6, Jimi E7, Hirata M8.  GLP-1 signaling is required for improvement of glucose tolerance by osteocalcin.  J Endocrinol  2020    PubMed ID: 31693486
11951  Liew LC, Gailhouste L, Tan GC, Yamamoto Y, Takeshita F, Nakagama H, Ochiya T.  MicroRNA-124a inhibits endoderm lineage commitment by targeting Sox17 and Gata6 in mouse embryonic stem cells.  Stem Cells  2020    PubMed ID: 31828873
12082  Kobayashi S, Ikeda Y, Shigeno Y, Konno H, Fujii J.  γ-Glutamylcysteine synthetase and γ-glutamyl transferase as differential enzymatic sources of γ-glutamylpeptides in mice.  Amino Acids  2020    PubMed ID: 32170467
12116  Noguchi T, Ikeda M, Murakami M, Masuzawa M, Imamura T, Hashimoto O, Matsui T, Funaba M.  Regulatory expression of bone morphogenetic protein 6 by 2,2'-dipyridyl.  Biochim Biophys Acta Gen Subj  2020    PubMed ID: 32251709
13060  Mayama S, Hamazaki N, Maruyama Y, Matsubara S, Kimura AP.  Transcriptional activation of the mouse Scd2 gene by interdependent enhancers and long noncoding RNAs in ovarian granulosa cells.  J Reprod Dev  2020    PubMed ID: 32507774
13073  Teratani T, Tomita K, Toma-Fukai S, Nakamura Y, Itoh T, Shimizu H, Shiraishi Y, Sugihara N, Higashiyama M, Shimizu T, Inoue I, Takenaka Y, Hokari R, Adachi T, Shimizu T, Miura S, Kanai T.  Redox-dependent PPARγ/Tnpo1 complex formation enhances PPARγ nuclear localization and signaling.  Free Radic Biol Med  2020    PubMed ID: 32553752
13090  Homma T, Kobayashi S, Fujii J.  Cysteine preservation confers resistance to glutathione-depleted cells against ferroptosis via CDGSH iron sulphur domain-containing proteins (CISDs).  Free Radic Res  2020    PubMed ID: 32605459
13566  Kim DH, Sadakane H, Nishikiori Y, Matsumura M, Ikeda M, Diao Z, Jha R, Murakami M, Matsui T, Funaba M.  Factors affecting expression and transcription of uncoupling protein 2 gene  J Vet Med Sci  2020  82(12):1734-1741  PubMed ID: 33162463
14835  Yamada T, Tateishi R, Iwai M, Koike K, Todo T.  Neoadjuvant Use of Oncolytic Herpes Virus G47Δ Enhances the Antitumor Efficacy of Radiofrequency Ablation  Mol Ther Oncolytics  2020  18:535-54  PubMed ID: 32995479
11550  Shigeto H, Ono T, Ikeda T, Hirota R, Ishida T, Kuroda A, Funabashi H.  Insulin sensor cells for the analysis of insulin secretion responses in single living pancreatic β cells.  Analyst  2019    PubMed ID: 31089611
11603  Aoki N, Matsui Y.  Comprehensive Analysis of Mouse Cancer/Testis Antigen Functions in Cancer Cells and Roles of TEKT5 in Cancer Cells and Testicular Germ Cells.  Mol. Cell. Biol.  2019    PubMed ID: 31208979
11769  Homma T, Kobayashi S, Fujii J.  Induction of ferroptosis by singlet oxygen generated from naphthalene endoperoxide.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31445701
11782  Homma T, Kobayashi S, Sato H, Fujii J.  Edaravone, a free radical scavenger, protects against ferroptotic cell death in vitro.  Exp. Cell Res.  2019    PubMed ID: 31479686
9192  Toru Suzuki, Chisato Kikuguchi, Saori Nishijima, Takeshi Nagashima, Akinori Takahashi, Mariko Okada, Tadashi Yamamoto  Postnatal Liver Functional Maturation Requires Cnot Complex-Mediated Decay of mRNAs Encoding Cell Cycle and Immature Liver Genes  Development  2019  146 (4)  PubMed ID: 30733279
10525  Mizunoe Y, Kobayashi M, Sudo Y, Watanabe S, Yasukawa H, Natori D, Hoshino A, Negishi A, Okita N, Komatsu M, Higami Y.  Trehalose protects against oxidative stress by regulating the Keap1-Nrf2 and autophagy pathways.  Redox Biol  2018  15:115-124  PubMed ID: 29241092
10795  Matsushita J, Okamura K, Nakabayashi K, Suzuki T, Horibe Y, Kawai T, Sakurai T, Yamashita S, Higami Y, Ichihara G, Hata K, Nohara K.  The DNA methylation profile of liver tumors in C3H mice and identification of differentially methylated regions involved in the regulation of tumorigenic genes.  BMC Cancer  2018  18:317  PubMed ID: 29566670
11103  Tsurudome Y, Koyanagi S, Kanemitsu T, Katamune C, Oda M, Kanado Y, Kato M, Morita A, Tahara Y, Matsunaga N, Shibata S, Ohdo S.  Circadian clock component PERIOD2 regulates diurnal expression of Na+/H+ exchanger regulatory factor-1 and its scaffolding function.  Sci Rep  2018  8(1):9072  PubMed ID: 29899468
11100  Nakano R, Ohira M, Yano T, Imaoka Y, Tanaka Y, Ohdan H.  Hepatic irradiation persistently eliminates liver resident NK cells.  PLoS ONE  2018  13(6):e0198904  PubMed ID: 29897952
11157  Germond A, Ichimura T, Chiu LD, Fujita K, Watanabe TM, Fujita H.  Cell type discrimination based on image features of molecular component distribution.  Sci Rep  2018  8(1):11726  PubMed ID: 30082723
4217  Shinto S, Matsuoka Y, Yamato M, Yamada KI.  Antioxidant nitroxides protect hepatic cells from oxidative stress-induced cell death.  J Clin Biochem Nutr  2018    PubMed ID: 29610552
10033  Nishihara M, Kanda GN, Suzuki T, Yamakado S, Harashima H, Kamiya H.  Enhanced transgene expression by plasmid-specific recruitment of histone acetyltransferase.  J. Biosci. Bioeng.  2017  123:277-280  PubMed ID: 27746157
10211  Fujita K, Iguchi Y, Une M, Watanabe S.  Ursodeoxycholic Acid Suppresses Lipogenesis in Mouse Liver: Possible Role of the Decrease in β-Muricholic Acid, a Farnesoid X Receptor Antagonist.  Lipids  2017  52:335-344  PubMed ID: 28315136
10364  Lee J, Homma T, Fujii J.  Mice in the early stage of liver steatosis caused by a high fat diet are resistant to thioacetamide-induced hepatotoxicity and oxidative stress.  Toxicol. Lett.  2017  277:92-103  PubMed ID: 28642009
10583  Lee J, Kang ES, Kobayashi S, Homma T, Sato H, Seo HG, Fujii J.  The viability of primary hepatocytes is maintained under a low cysteine-glutathione redox state with a marked elevation in ophthalmic acid production.  Exp. Cell Res.  2017  361:178-191  PubMed ID: 29079265
7067  Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M.  Fluorescence probes to detect lipid-derived radicals.  Nat. Chem. Biol.  2016  12:608-13  PubMed ID: 27294322
9733  Takahashi T, Sugawara W, Takiguchi Y, Takizawa K, Nakabayashi A, Nakamura M, Nagano-Ito M, Ichikawa S.  Identification of Plants That Inhibit Lipid Droplet Formation in Liver Cells: Rubus suavissimus Leaf Extract Protects Mice from High-Fat Diet-Induced Fatty Liver by Directly Affecting Liver Cells.  Evid Based Complement Alternat Med  2016  2016:4282758  PubMed ID: 27429636
12291  Saneyuki Ujino, Hironori Nishitsuji, Takayuki Hishiki, Kazuo Sugiyama, Hiroshi Takaku, Kunitada Shimotohno  Hepatitis C virus utilizes VLDLR as a novel entry pathway  Proc Natl Acad Sci U S A  2016  113(1):188-93  PubMed ID: 26699506
14124  Kobayashi M, Suzuki M, Ohno T, Tsuzuki K, Taguchi C, Tateishi S, Kawada T, Kim YI, Murai A, Horio F.  Detection of differentially expressed candidate genes for a fatty liver QTL on mouse chromosome 12  BMC Genet  2016  17(1):73  PubMed ID: 27266874
7330  Uyama T, Kawai K, Kono N, Watanabe M, Tsuboi K, Inoue T, Araki N, Arai H, Ueda N.  Interaction of Phospholipase A/Acyltransferase-3 with Pex19p: A POSSIBLE INVOLVEMENT IN THE DOWN-REGULATION OF PEROXISOMES.  J. Biol. Chem.  2015  290:17520-34  PubMed ID: 26018079
7395  Mutai H, Miya F, Fujii M, Tsunoda T, Matsunaga T.  Attenuation of progressive hearing loss in DBA/2J mice by reagents that affect epigenetic modifications is associated with up-regulation of the zinc importer Zip4.  PLoS ONE  2015  10:e0124301  PubMed ID: 25875282
15811  Ichi I, Kono N, Arita Y, Haga S, Arisawa K, Yamano M, Nagase M, Fujiwara Y, Arai H.  Identification of genes and pathways involved in the synthesis of Mead acid (20:3n-9), an indicator of essential fatty acid deficiency  Biochim Biophys Acta  2014  1841(1):204-13  PubMed ID: 24184513
15913  Kurihara M, Shiraishi A, Satake H, Kimura AP.  A conserved noncoding sequence can function as a spermatocyte-specific enhancer and a bidirectional promoter for a ubiquitously expressed gene and a testis-specific long noncoding RNA  J Mol Biol  2014  426(17):3069-93  PubMed ID: 25020229
15764  Burdeos GC, Nakagawa K, Watanabe A, Kimura F, Miyazawa T.  γ-Tocotrienol attenuates triglyceride through effect on lipogenic gene expressions in mouse hepatocellular carcinoma Hepa 1-6  J Nutr Sci Vitaminol (Tokyo)  2013  59(2):148-51  PubMed ID: 23727646
13154  Hideaki Yamamoto, Yoshinori Kawabe, Akira Ito, Masamichi Kamihira  Enhanced liver functions in mouse hepatoma cells by induced overexpression of liver-enriched transcription factor  Biochemical Engineering Journal  2012  60:67-73  
15762  Hamasaki T, Suzuki H, Shirohzu H, Matsumoto T, D'Alessandro-Gabazza CN, Gil-Bernabe P, Boveda-Ruiz D, Naito M, Kobayashi T, Toda M, Mizutani T, Taguchi O, Morser J, Eguchi Y, Kuroda M, Ochiya T, Hayashi H, Gabazza EC, Ohgi T.  Efficacy of a novel class of RNA interference therapeutic agents  PLoS One  2012  7(8):e42655  PubMed ID: 22916145
8206  Matsubara S, Takahashi T, Kimura AP.  Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression.  Gene  2010  465:17-29  PubMed ID: 20600704
8276  Miyayama T, Hiraoka D, Kawaji F, Nakamura E, Suzuki N, Ogra Y.  Roles of COMM-domain-containing 1 in stability and recruitment of the copper-transporting ATPase in a mouse hepatoma cell line.  Biochem. J.  2010  429:53-61  PubMed ID: 20433422
15367  Miki T, Iguchi M, Akiba K, Hosono M, Sobue T, Danbara H, Okada N.  Chromobacterium pathogenicity island 1 type III secretion system is a major virulence determinant for Chromobacterium violaceum-induced cell death in hepatocytes  Mol Microbiol  2010  77(4):855-72  PubMed ID: 20545857
3356  Kubota, Koichi  A novel functional T cell hybridoma recognizes macrophage cell death induced by bacteria: a possible role for innate lymphocytes in bacterial infection.  J Immunol  2006  176:7576-88  PubMed ID: 16751404
3336  Kuang M, Liu, S Q, Saijo K, Uchimura E, Huang L, Leong, K W, Lu, M D, Huang, J F, Ohno T  Microwave tumour coagulation plus in situ treatment with cytokine-microparticles: induction of potent anti-residual tumour immunity.  Int J Hyperthermia  2005  21:247-57  PubMed ID: 16019851
3189  Ogawa S, Tagawa Y, Kamiyoshi A, Suzuki A, Nakayama J, Hashikura Y, Miyagawa S.  Crucial roles of mesodermal cell lineages in a murine embryonic stem cell-derived in vitro liver organogenesis system.  Stem Cells  2005  23:903-13  PubMed ID: 16043458
9007  Emoto M, Miki M, Sarker AH, Nakamura T, Seki Y, Seki S, Ikeda S.  Structure and transcription promoter activity of mouse flap endonuclease 1 gene: alternative splicing and bidirectional promoter.  Gene  2005  357:47-54  PubMed ID: 15967598
2705  Kushida S, Peng, B G, Uchimura E, Kuang M, Huang L, Miwa M, Ohno T  A tumour vaccine of fixed tumour fragments in a controlled-release vehicle with cytokines for therapy of hepatoma in mice.  Dig Liver Dis  2004  36:478-85  PubMed ID: 15285528
2637  Kasahara M, Watanabe Y, Sumasu M, Nagata T  A family of MHC class I-like genes located in the vicinity of the mouse leukocyte receptor complex.  Proc Natl Acad Sci U S A  2002  99:13687-92  PubMed ID: 12370446
2519  Zhang BN, Watanabe S, Kohyama M, Saijo K, Kusakabe M, Ohno T.  Tumor formation suppressed in gammadeltaT knock-out mice.  Cancer Lett  2000  153(1-2):63-6  PubMed ID: 10779631

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