細胞番号 : 細胞名
RCB1635 : K-562
update : 2024/08/14
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細胞特性(Comment:英) | Human cell line derived from chronic myelogenous leukemia. Can produce hemoglobin in the presence of NaB. Directly deposited from the institution that has established this cell line. |
細胞特性(日) | |
細胞特性(寄託者記述:英) | |
細胞特性(寄託者記述:日) | ヒト慢性骨髄性白血病由来細胞株。NaBの添加によりヘモグロビンを産生する。本細胞株を樹立した機関から直接入手した株。 |
使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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提供申込書類(日) |
依頼書C-0001.pdf
 
同意書(非営利学術目的)C-0003.pdf
 
同意書(営利目的)C-0003p.pdf
 
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
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寄託者 |
Ichiki, T. Albert
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樹立者 |
Lozzio, C.B. & Lozzio, B.B.
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寄託日 |
2000
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動物種 |
_human
< Mammals
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属名 |
Homo
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種名 |
sapiens
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性別 |
Female
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齢 |
53 years
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採取組織 |
pleural effusion
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病名 |
chrinic myelogenous leukemia
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細胞分類 |
cancer
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入手歴 |
overseas
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細胞寿命 |
infinite
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細胞形態 |
lymphoblast-like
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Cellosaurus(Expasy) |
CVCL_0004
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Suspension cells
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培養方法 |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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培地 |
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MEM + 15% FBS + 0.1mM NEAA
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抗生物質 |
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Free
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継代方法 |
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dilution
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播種細胞数 |
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1.5 x 10 4 cells/ml
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継代密度 |
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1 : 10 split
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継代・培地交換頻度 |
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Subculture : 2 times/week
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培養最適温度 |
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37
℃
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二酸化炭素濃度 |
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5
%
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凍結培地 |
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Medium + 5% Glycerol
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凍結方法 |
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Slow freezing
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マイコプラズマ |
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(-)
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ウィルス(HIV) |
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LT 400
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ウィルス(HTLV-1) |
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(-)
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アイソザイム検査 |
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LD, NP
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染色体検査 |
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62-67(50) : /63(3),64(8),65(33),66(4),67(1)
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個体識別検査 |
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OK
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画像情報 |
寄託時情報 | ロット情報 |
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文献情報 |
Reference(英) |
31件
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Reference(日) |
1件
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利用者成果(英) |
4件
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利用者成果(日) |
0件
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Reference(英) |
117
Baker EJ, Ichiki AT, Day NE, Andrews RB, Bamberger EG, Lozzio CB.
Simultaneous flow cytometric measurement of K-562 megakaryocytic differentiation and CD56+ large granular lymphocyte cytotoxicity
J Immunol Methods
2001
253(1-2):37-44
PubMed ID: 11384667
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98
Baker EJ, Ichiki AT, Hodge JW, Sugantharaj D, Bamberger EG, Lozzio CB.
PMA-treated K-562 leukemia cells mediate a TH2-specific expansion of CD4+ T cells in vitro
Leuk Res
2000
24(12):1049-57.
PubMed ID: 11077119
DOI: 10.1016/s0145-2126(00)00082-5
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97
Ichiki AT, Langenberg M, Baker EJ, Hodge JW, Bamberger EG, Gerard DA, Lozzio CB.
Differential regulation of interleukin-1alpha and interleukin-1beta in K-562 cells
J Interferon Cytokine Res
1998
18(12):1045-50
PubMed ID: 9877448
DOI: 10.1089/jir.1998.18.1045
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101
Wust CJ, Hodge JW, Ichiki AT, Lozzio CB.
Cell death in the human leukemia cell line, K-562, induced by antiserum and monoclonal antibodies
Leuk Res
1991
15(6):497-506
PubMed ID: 1713636
DOI: 10.1016/0145-2126(91)90061-w
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103
Ichiki AT, Sugantharaj DG, Hudson CS, Gerard DA, Barnhill MA, Bamberger EG, Wust CJ, Fuhr JE, Lozzio CB.
Lowered susceptibility of K-562 cells treated with gamma interferon in serum-free medium to natural killer cell-mediated cytolysis
Nat Immun Cell Growth Regul
1988
7(5-6):297-304
PubMed ID: 3146698
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106
Walter H, Krob EJ, Al-Romaihi FA, Johnson D, Lozzio CB.
Detection of surface differences between closely related cell populations by partitioning. Cultured K-562 cell sublines
Cell Biophys
1988
13(3):173-87
PubMed ID: 2465826
DOI: 10.1007/BF02918374
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5042
Haruta Y, Seon BK.
Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.
Proc Natl Acad Sci U S A
1986
83(20):7898-902
PubMed ID: 3464004
DOI: 10.1073/pnas.83.20.7898
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95
Ichiki AT, Bamberger EG, Wust CJ, Lozzio CB.
Diversity of cell surface hematopoietic antigens on K-562 sublines identified with monoclonal antibodies
Leuk Res
1986
10(5):565-74
PubMed ID: 2940421
DOI: 10.1016/0145-2126(86)90092-5
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96
Ichiki AT, Wust CJ, Lozzio CB.
Characterization of the Fc(IgG) receptor on the pluripotential leukaemia cell K-562
Clin Exp Immunol
1985
59(1):64-8
PubMed ID: 3971599
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116
Rowley PT, Ohlsson-Wilhelm BM, Wisniewski L, Lozzio CB, Lozzio BB.
K562 human leukemia cell passages differ in embryonic globin gene expression
Leuk Res
1984
8(1):45-54
PubMed ID: 6199625
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100
Lozzio BB, Machado EA, Mitchell J, Lozzio CB, Wust CJ, Golde DW.
Proliferation of human malignant hematopoietic cells in immunodeficient mice: suppression by antibody to pluripotent K-562 leukemia cells involves direct cytolysis and effector cells
Blood
1983
61(6):1045-53
PubMed ID: 6132631
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94
Wust CJ, Green M, Lozzio CB, Lozzio BB.
Antibody-dependent cytolysis of the human leukemia cell line K-562 in the absence of effector cells or complement
Blood
1982
59(1):133-40
PubMed ID: 7053760
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102
Ichiki AT, Lozzio BB, Wust CJ, Lozzio CB.
Relationship between the Fc receptor for IgG and the specific associated antigen of the pluripotential leukaemia cell line, K-562
Clin Exp Immunol
1982
50(2):360-5
PubMed ID: 6185259
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105
Machado EA, Gerard DA, Mitchell JR, Lozzio BB, Lozzio CB.
Arrest and extravasation of neoplastic cells. An electron microscopy study of serial sections at sequential stages
Virchows Arch A Pathol Anat Histol
1982
396(1):73-89
PubMed ID: 7123846
DOI: 10.1007/BF00428501
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107
Lozzio CB, Lozzio BB, Wust CJ, Bamberger EG, Golde DW, Minowada J.
Antibody lysis of human hematopoietic cells in the absence of complement and effector cells
Exp Hematol
1982
10(8):698-707
PubMed ID: 6183138
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115
Perez RL, Mitchell JR, Lozzio BB.
Relevance of lipids to heterotransplantation of human malignancies
Oncology
1982
39(3):179-84
PubMed ID: 7043353
DOI: 10.1159/000225632
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87
Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS.
A multipotential leukemia cell line (K-562) of human origin
Proc Soc Exp Biol Med
1981
166(4):546-50
PubMed ID: 7194480
DOI: 10.3181/00379727-166-41106
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93
Fuhr JE, Bamberger EG, Lozzio CB, Lozzio BB.
Induction of hemoglobin synthesis in original K 562 cell line
Blood Cells
1981
7(2):389-99
PubMed ID: 6170369
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108
Latif ZA, Lozzio BB, Wust CJ, Krauss S, Aggio MC, Lozzio CB.
Evaluation of drug-antibody conjugates in the treatment of human myelosarcomas transplanted in nude mice
Cancer
1980
45(6):1326-33
PubMed ID: 6928397
DOI: 10.1002/1097-0142(19800315)45:6<1326::aid-cncr2820450610>3.0.co;2-y
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113
Lair SV, Lozzio BB.
Thymocyte reconstitution of athymic and athymic-asplenic mice: graft rejection and antibody synthesis
Exp Cell Biol
1980
48(6):439-44
PubMed ID: 6967835
DOI: 10.1159/000163010
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969
Rutherford TR, Clegg JB, Weatherall DJ.
K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.
Nature
1979
280(5718):164-5
PubMed ID: 95354
DOI: 10.1038/280164a0
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970
Andersson LC, Jokinen M, Gahmberg CG.
Induction of erythroid differentiation in the human leukaemia cell line K562.
Nature
1979
278(5702):364-5
PubMed ID: 570644
DOI: 10.1038/278364a0
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89
Lozzio BB, Machado EA, Lair SV, Lozzio CB.
Reproducible metastatic growth of K-562 human myelogenous leukemia cells in nude mice
J Natl Cancer Inst
1979
63(2):295-9
PubMed ID: 287825
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112
Lozzio CB, Lozzio BB, Wust CJ, Kim J.
Correlation of leukemia-associated antigens and Ph1 chromosome in fibroblastlike cells derived from bone marrow
Blood
1978
52(4):673-80
PubMed ID: 99197
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114
Lozzio BB, Lozzio CB, Bamberger EG.
Cytotoxicity of a factor from normal and abnormal human spleens for allogenic leukemia cells
Am J Hematol
1978
4(1):57-65
PubMed ID: 655158
DOI: 10.1002/ajh.2830040108
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99
Collins JL, Wust CJ, Lozzio BB, Lozzio CB.
Characterization of an antigen from the myelogenous leukemia cell line K-562
J Natl Cancer Inst
1977
59(6):1667-74
PubMed ID: 72824
DOI: 10.1093/jnci/59.6.1667
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109
Lozzio BB, Lozzio CB, Krauss S, Wust CJ, Girardi A.
Leukemia-associated antigens detected by a nonhuman primate antiserum to a Ph1+ myeologenous leukemia cell line
Blood
1977
50(1):115-24
PubMed ID: 68791
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111
Lozzio BB, Machado EA, Lair SA, Lozzio CB.
Suppression of human myelosarcoma growth in athymic mice by a primate antiserum
Cancer Treat Rep
1977
61(9):1679-84
PubMed ID: 271518
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88
Lozzio CB, Lozzio BB, Yang WK, Ichiki AT, Bamberger EG.
Absence of thymus-derived lymphocyte markers in myelogenous leukemia (Ph1+) cell line K-562
Cancer Res
1976
36(12):4657-62
PubMed ID: 63324
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104
Lozzio BB, Lozzi CB, Machado E.
Human myelogenous (Ph+) leukemia cell line: transplantation into athymic mice
J Natl Cancer Inst
1976
56(3):627-9
PubMed ID: 1062625
DOI: 10.1093/jnci/56.3.627
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110
Lozzio BB, Machado EA, Lozzio CB, Lair S.
Hereditary asplenic-athymic mice: transplantation of human myelogenous leukemic cells
J Exp Med
1976
143(1):225-31
PubMed ID: 53268
DOI: 10.1084/jem.143.1.225
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