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Cell No. : Cell Name
RCB1635 : K-562  update : 2022/04/05
CommentHuman CML derived cell line. Deposited directly from the institution that has established this cell.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ichiki, T. Albert
Originator Lozzio, C.B. & Lozzio, B.B.
Year of deposit 2000
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 53 years
Tissue pleural effusion
Disease name chrinic myelogenous leukemia
Classification cancer
History overseas
Lifespan infinite
Morphology lymphoblast-like
Cellosaurus(Expasy) CVCL_0004
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives MEM + 15% FBS + 0.1mM NEAA
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5 x 10^(4) cells/ml
Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 5% Glycerol
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 62-67(50) : /63(3),64(8),65(33),66(4),67(1)
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 3

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5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004
969  Rutherford TR, Clegg JB, Weatherall DJ.  K562 human leukaemic cells synthesise embryonic haemoglobin in response to haemin.  Nature  1979  280(5718):164-5  PubMed ID: 95354
970  Andersson LC, Jokinen M, Gahmberg CG.  Induction of erythroid differentiation in the human leukaemia cell line K562.  Nature  1979  278(5702):364-5  PubMed ID: 570644

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User's Publication
12492  Aoyama T, Shibayama Y, Furukawa T, Sugawara M, Takekuma Y.  Continuous Cytostatic Effects of BCR-ABL Tyrosine Kinase Inhibitors (TKIs) after Washout in Human Leukemic K562 Cells  Biol Pharm Bull  2019  42(11):1805-1813  PubMed ID: 31434819
13861  Tazawa Y, Usukubo I, Takada K, Takekuma Y, Shibayama Y, Sugawara M.  Schedule-dependent cytotoxicity of Etoposide and cyclophosphamide in P-glycoprotein-expressing human leukemic K-562 cells  Biol Pharm Bull  2014  37(8):1323-9  PubMed ID: 25087953
13862  Tazawa Y, Matsumura K, Takekuma Y, Sugawara M.  Schedule-dependent cytotoxicity of etoposide (VP-16) and cyclophosphamide in leukemia cell line K-562  Biol Pharm Bull  2012  35(7):1132-6  PubMed ID: 22791162

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