Cell No. : Cell Name
RCB1633 : ΔMre11ΔKu70-DT40
update : 2024/01/17
|
| Comment | Mre11 and Ku70 gene double-deleted DT40 (chicken B cells). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Takeda, Shunichi
|
| Originator |
Takeda, Shunichi
|
| Year of deposit |
2000
|
| Another name |
#273
|
| Original cell |
DT40
|
| Animal |
_avian
< Birds
|
| Tissue |
B cell
|
| Classification |
mutant
|
| Recombinant |
recombinant
|
| Exogene |
Mre-tet, Ku70-his, Ku-70-brs
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_4Z66
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage cell No |
|
1.5-2 x 10 5 cells/ml
|
| Passage ratio |
|
1 : 20 split
|
| SC frequency |
|
Subculture : every 2 days
|
| Temperature |
|
39.5
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Others |
|
Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
17849
Takeda S, Hoa NN, Sasanuma H.
The role of the Mre11-Rad50-Nbs1 complex in double-strand break repair-facts and myths
J Radiat Res
2016
57 Suppl 1(Suppl 1):i25-i32
PubMed ID: 27311583
DOI: 10.1093/jrr/rrw034
|
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3435
Mizutani A, Okada T, Shibutani S, Sonoda E, Hochegger H, Nishigori C, Miyachi Y, Takeda S, Yamazoe M.
Extensive chromosomal breaks are induced by tamoxifen and estrogen in DNA repair-deficient cells.
Cancer Res.
2004
64(9):3144-7
PubMed ID: 15126352
DOI: 10.1158/0008-5472.can-03-3489
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2740
Yamaguchi-Iwai Y, Sonoda E, Sasaki MS, Morrison C, Haraguchi T, Hiraoka Y, Yamashita YM, Yagi T, Takata M, Price C, Kakazu N, Takeda S
Mre11 is essential for the maintenance of chromosomal DNA in vertebrate cells.
EMBO J
1999
18:6619-29
PubMed ID: 10581236
DOI: 10.1093/emboj/18.23.6619
|
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